LU Chun-bin,ZHENG Jian-xin,CAI Juan,et al.Effects of Genetically Modified Soybeans on Embryonic Development and the Ability of Fertilization in Female Mice[J].Soybean Science,2014,33(04):578-582.[doi:10.11861/j.issn.1000-9841.2014.04.0578]
转基因大豆对雌鼠胚胎发育及受精能力的影响
- Title:
- Effects of Genetically Modified Soybeans on Embryonic Development and the Ability of Fertilization in Female Mice
- Keywords:
- Genetically modified soybeans; Estrous cycle; Ability of fertilization; Embryonic development; Reproductive toxicity
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 为评估转基因大豆对雌鼠胚胎发育及受精能力的影响，用转基因大豆饲料（试验组）与非转基因大豆饲料（对照组）分别喂食雌鼠，30和90 d后分别检测观察动情周期变化规律及受精卵、2-细胞胚胎、囊胚在体内的发育情况。结果显示：试验组与对照组雌鼠动情周期均在4~5 d范围内，无显著差异；与对照组相比，试验组雌鼠体内受精的合子（受精卵、2-细胞胚胎、囊胚）数量、受精率、优胚率无显著性差异。研究结果表明：短期（30 d）和长期（90 d）喂食转基因大豆不会对雌鼠动情周期、受精能力及早期胚胎发育发生不良影响。
- Abstract:
- The objective of present study was to evaluate the effects of genetically modified soybeans(GM)on embryonic development and the ability of fertilization in female mice.In present study,the female mice were fed genetically modified soybean diet or conventional soybean diet for 90 days,respectively.At 30 and 90 days of feeding trial,the changes of estrous cycle and development of the zygotes,2-cell embryos,blastocysts in mice of two groups were observed.There were no significant differences in estrous cycle,usually varied from 4 to 5 days.Compared with conventional diet,there were no significant differences in the number of zygotes,2-cell embryos,blastocysts,and fertilization rate,as well as the rate of high qualified embryo in female mice fed GM soybean diet.The results of feeding revealed that there were no adverse effects on the estrous cycle and the ability of fertilization,embryonic development of female mice,suggesting no potential reproductive toxicity on female mice fed genetically modified soybeans.
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备注/Memo
基金项目：转基因生物新品种培育重大专项课题（2014ZX08012-005）。