SONG Zhi-feng,NIU Hong-hong,HE Zhi-yong,et al.Analysis of Volatile Compounds in Soybean Flowers by Static Headspace-GC-MS[J].Soybean Science,2014,33(04):570-573.[doi:10.11861/j.issn.1000-9841.2014.04.0574]
静态顶空萃取-气相色谱-质谱法分析大豆花中挥发性成分
- Title:
- Analysis of Volatile Compounds in Soybean Flowers by Static Headspace-GC-MS
- Keywords:
- Static headspace; GC-MS; Soybean flowers; Volatile compounds
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用静态顶空萃取-气相色谱-质谱联用技术(GC-MS)建立了大豆花挥发性成分分离鉴定方法。大豆花样品中挥发性成分经静态顶空进样器提取,由气相色谱分离、质谱检测,NIST质谱数据库检索定性鉴定;面积归一化法定量测定。从5种大豆花中共分离鉴定出31种挥发性化合物,这些化合物主要为醇、酮、醛、烃、酯和呋喃类。其中对大豆花气味特征的主要贡献组分为醇和酮类化合物。不同品种大豆花中挥发性成分含量存在较大差异。
- Abstract:
- In order to study components difference in flowers of 5 soybean cultivars,a method used to isolate and identify the volatile compounds from soybean flowers by static headspace extraction coupled to gas chromatography-mass spectrometry(GC-MS)was set up.The volatile components were extracted by static headspace,isolated by CC,qualitative identified by NIST spectrum library index combining with literature,and quantitative determined by area normalization method.Thrty-one volatile compounds were isolated and identified from 5 soybean cultivars and they belongto alcohol,ketone,aldehyde,hydrocarbon,ester,and furancompounds.The main volatile compounds of soybean flower were alcohol and ketone.The content difference of the volatile compounds in flowers of different soybean cultivars was remarkable.These results would provide important reference for the research attraction of natural enemy insects and resource development of pollination insect.
参考文献/References:
[1]赵丽梅,彭宝,张伟龙,等,杂交大豆制种技术体系的建立[J].大豆科学,2010,29(4):707-711.(Zhao L M,Peng B,Zhang W L,et al.Establishment of technology system for hybrid soybean seed production[J].Soybean Science,2010,29(4):707-711. [2] 邱丽丽,容蓉,张莹,等.水蒸气蒸馏与顶空进样GC-MS分析白胡椒挥发性成分[J].食品科学,2010,31(14):161-164.(Qiu L L,Rong R,Zhang Y,et al.Steam distillation or headspace extraction coupled to gas chromatography-mass spectrometry for the volatile composition analysis of white pepper[J].Food Science,2010,31(14):161-164.) [3] Gan J Y,Sharon P,Scott R Y.Static headspace and gas chromatographic analysis of fumigant residues in soil and water[J].Journal of Agricultural and Food Chemistry,1988,46:986-990. [4] 李英华,吕秀阳,李准,等.顶空气相色谱法在特产气味成分分析鉴定中的应用研究[J].天然产物研究与开发,2005,17(增刊):121-125.(Li Y H,Lyu X Y,Li Z,et al.Application of headspace gas chromatography in quality appraisal and scent components of special products[J].Natural Product Research and Development,2005,17(Suppl.):121-125.) [5] Guevas-Glory L F,Jorge A P,Louis S S,et al.A review of volatile analytical methods for determining the botanical origin of honey[J].Food Chemistry,2007,103:1032-1043. [6] 邓晓军,杜家纬.采用多次顶空固相微萃取分析拟南芥绿叶挥发性物质[J].生态学杂志,2005,24(8):970-974.(Deng X J,Du J W.Multiple headspace solid phase micro-extraction and analysis of volatiles from Arabidopsisgreen leaf[J].Chinese Journal of Ecology,2005,24(8):970-974.) [7] 王建新,朱同胜.食用香料1-辛烯-3-醇的合成[J].无锡轻工大学学报,1998,17(1):89-91.(Wang J X,Zhu T S.The synthesis of edible fragrance 1-octen-3-ol[J].Journal of Wuxi University of Light Industry,1998,17(1):89-91.) [8] 卓成龙,李大婧,宋江峰,等.微波烫漂与热水烫漂毛豆仁风味成分比较[J].食品科学,2010,31(18):321-324.(Zhuo C L,Li D J,Song J F,et al.Comparison of the flavor of green soybean with microwave and hot water blanching[J].Food Science,2010,31(18):321-324.) [9] 刘健,马凤鸣,赵奎军.大豆植株挥发性成份定性分析[J].大豆科学,2009,28(4):719-722.(Liu J,Ma F M,Zhao K J.Component analysis of volatile compounds released from soybean[J].Soybean Science,2009,28(4):719-722.) [10]王璐.养殖草鱼肉主体气味及其环境影响因素研究[D].上海:上海海洋大学,2009.(Wang L.Effect of environmental impact factors on key odor compounds of cultured grass carp meat[D].Shanghai: Shanghai Ocean University,2009.) [11]沈丽,张丽君,许柏球.不同解冻时间对冰鲜牡蛎挥发性风味物质的影响[J].肉类研究,2012,26(3):5-8.(Shen L,Zhang L J,Xu B Q.Change in volatile flavor components of frozen oyster during thawing[J].Meat Research,2012,26(3):5-8.) [12]邹建凯.猪油挥发油成分的气相色谱/质谱法分析[J].分析化学,2002,30(4):512.(Zhou J K.Analysis of lard essential oil composition by gas chromatography/mass spectrometry[J].Chinese Journal of Analytical Chemistry,2002,30(4):512.) [13]陈展册,苏丽,戈峰,等.绿盲蝽对性激素类似物和植物挥发物的触角电位反应[J].昆虫学报,2010,53(1):47-54.(Chen Z C,Su L,Ge F,et al.Electroantennogram responses of the green leaf bug,Lygus lucorummeyer-Dür(Hemiptera:Miridae),to sex pheromone analogs and plant volatiles[J].Acta Entomologica Sinica,2010,53(1):47-54.) [14]黄显慈.鉴别豆油回味产生强烈气味的化合物[J].粮食加工,1990(2):41-45.(Huang X C.Identification of soybean oil with a strong odor compounds[J].Grain Processing,1990(2):41-45.)
备注/Memo
第一作者简介:宋志峰(1975-),男,副研究员,学士,主要从事农产品质量安全检测与风险评估技术研究。E-mail:mhsr@sina.com。