[1]王 浩,姜 妍,刘 伟,等.大豆根际高效溶磷菌株的分离及溶磷能力分析[J].大豆科学,2014,33(03):404-407.[doi:10.11861/j.issn.1000-9841.2014.03.0404]
　WANG Hao,JIANG Yan,LIU Wei,et al.Isolation and Analysis on Ability of Phosphate Dissolving Bacteria in Rhizosphere of Soybean[J].Soybean Science,2014,33(03):404-407.[doi:10.11861/j.issn.1000-9841.2014.03.0404]
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大豆根际高效溶磷菌株的分离及溶磷能力分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年03期 页码: 404-407 栏目: 出版日期: 2014-06-25
Title:
Isolation and Analysis on Ability of Phosphate Dissolving Bacteria in Rhizosphere of Soybean
作者:
王 浩; 姜 妍; 刘 伟; 李远明; 于 佳; 王绍东
东北农业大学 国家大豆工程技术研究中心，黑龙江 哈尔滨 150028
Author(s):
WANG Hao; JIANG Yan; LIU Wei; LI Yuan-ming; YU Jia; WANG Shao-dong
National Research Center of Soybeen Engineering and Technology,Northeast Agricultural University,Harbin 150086,China
关键词:
大豆; 根际; 溶磷菌; 溶磷能力
Keywords:
Soybean; Rhizosphere; Phosphate dissolving bacteria; Phosphate dissolving ability
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0404
文献标志码:
A
摘要:
采用无机磷合成培养基从大豆根际分离到223株细菌，其中能够产生明显溶磷圈的菌株47株，且在土壤中添加1%Ca3(PO4)2有利于筛选获得具有高效溶磷能力的菌株。对菌株的溶磷量进行测定，测得菌株的溶磷量为51.64～199.20 mg.L-1，对溶磷能力最强的菌株SR 95进行平板传代，并不断提高培养基中Ca3(PO4)2含量，可使菌株的溶磷量从199.20 mg?L-1提高到243.71 mg.L-1。对分离到溶磷能力较强的13株菌进行16S rDNA部分序列测定，经在线比对并构建系统发育树，发现其分布在7个不同种当中，其中溶磷能力最强的菌株SR95为成团泛菌，这在以往研究中未见报道。
Abstract:
By using inorganic phosphate synthetic culture media,223 strains of inoganic phosphate dissolving bacteria were isolated from soybean rhizoshpere,47 strains could produce dissolving phosphate zone among them,and adding 1% Ca3(PO4)2 to soil was benefit to isolate strains with high ability of dissolving phosphate.The dissolving inorganic phosphate quantities measured were ranged from 51.64~199.20 mg.L-1.Passage cultured the most powerful dissolving phosphate strain SR95 and improved the content of Ca3(PO4)2in the media continuously,the dissolving inorganic phosphate quantities were improved from 199.20 to 243.71 mg.L-1.Sequenced partial 16S rDNA of the more powerful dissolving phosphate strains,nucleic acid sequence was blasted in GenBank online and phylogenetic tree was constructed.The result showed that all strains were in 7 different species,the most powerful dissolving phosphate strain SR 95 was identified as Pantoea agglomerans,which was not reported as dissolving phosphate bacteria previously.

参考文献/References:

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备注/Memo

收稿日期：2013-11-21

基金项目：黑龙江省青年科学基金（QC2013C013）；哈尔滨市科技局青年科技创新人才（2013RFQQJ173）；东北农业大学大豆生物学教育部重点实验室开放基金（SB12B03）；东北农业大学博士启动基金（2010RCB72）。
第一作者简介：王浩（1980-），女，博士，助理研究员，主要从事土壤微生物研究。E-mail:gallian2006@aliyun.com。
通讯作者：王绍东（1966-），男，博士，副研究员，主要从事大豆品质育种研究。E-mail:wsdhlj@aliyun.com。

更新日期/Last Update: 2014-08-01