[1]梁喜龙,李国兰,崔洪秋,等.大豆DNA去甲基化酶ROS1的生物信息学分析[J].大豆科学,2014,33(03):321-324.[doi:10.11861/j.issn.1000-9841.2014.03.0321]
　LIANG Xi-long,LI Guo-lan,CUI Hong-qiu,et al.Bioinformatics Analysis of DNA Demethylase ROS1 in Soybean[J].Soybean Science,2014,33(03):321-324.[doi:10.11861/j.issn.1000-9841.2014.03.0321]
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大豆DNA去甲基化酶ROS1的生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年03期 页码: 321-324 栏目: 出版日期: 2014-06-25
Title:
Bioinformatics Analysis of DNA Demethylase ROS1 in Soybean
作者:
梁喜龙1; 李国兰1; 崔洪秋1; 鞠世杰1; 洪艳华1; 方淑梅2; 郑殿峰1
1.黑龙江八一农垦大学 农学院，黑龙江 大庆 163319； 2.黑龙江八一农垦大学 生命学院，黑龙江 大庆 163319
Author(s):
LIANG Xi-long1; LI Guo-lan1; CUI Hong-qiu1; JU Shi-jie1; HONG Yan-hua1; FANG Shu-mei2; ZHENG Dian-feng1
1.Agronomy College,Heilongjiang Bayi Agricultural University,Daqing 163319,China;2.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China
关键词:
大豆; DNA去甲基化酶; ROS1; 生物信息学分析
Keywords:
Soybean; DNA demethylase; ROS1; Bioinformatics analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0321
文献标志码:
A
摘要:
利用NCBI、Phytozome、ExPASy等网站及其数据库，初步确定了大豆DNA去甲基化酶ROS1的蛋白及基因序列、基因拷贝数、理化特性等，并进一步预测分析了二级结构及结构域，同时结合Clustal X2.0和MEGA4.0等软件进行多重序列比对和分子系统进化关系研究。结果显示，大豆ROS1包括6个拷贝，ROS1各拷贝的分子量相近，等电点酸性，具有相似的潜在磷酸化位点，属于不稳定的疏水性蛋白，都位于细胞核内，且不含信号序列。α螺旋是主要的二级结构，均含有DNA_glycosylase，HhH-GPD_domain和HTH_base_excis_C三个结构域。
Abstract:
Websites and data bases were employed such as NCBI,Phytozome and ExPASy in this paper to identify the sequences of proteins and genes,gene copies and some physicochemical properties of ROS1 in soybean.Its secondary structure and domain were predicted and analyzed.Meanwhile,phylogenetic tree was constructed based on the sequences of amino acids to analyze the revolutionary relations among different species.The results showed that ROS1 included six copies with similar molecular weight in soybean.There were acid isoelectric points and similar potential phosphorylation sites in the ROS1 proteins but signal sequences were lacked.All copies were all unstable and hydrophobic and were expressed in nucleus.α helix was the main secondary structure.DNA_glycosylase，HhH-GPD_domain and HTH_base_excis_C domains were included in the ROS1 proteins of soybean.

参考文献/References:


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备注/Memo

收稿日期：2013-11-05

基金项目：黑龙江省教育厅科学技术研究项目（12521381）。
第一作者简介：梁喜龙(1976-)，男，在读博士，副教授，主要从事植物化控及生物逆境方面研究。E-mail：xilongliang@126.com。
通讯作者：郑殿峰（1969-），男，教授，博士生导师，主要从事大豆化控及生物逆境方面研究。E-mail：zdffnj@263.net。

更新日期/Last Update: 2014-08-01