[1]林凡敏,柏锡,樊超,等.转GsGST14耐盐碱基因大豆的农艺性状调查[J].大豆科学,2013,32(01):56-62.[doi:10.3969/j.issn.1000-9841.2013.01.013]
　LIN Fan-min,BAI Xi,FAN Chao,et al.Investigation and Analysis of the Main Agronomic Traits of Different Transgenic Soybean Lines with GsGST14 Gene[J].Soybean Science,2013,32(01):56-62.[doi:10.3969/j.issn.1000-9841.2013.01.013]
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转GsGST14耐盐碱基因大豆的农艺性状调查

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第32卷 期数: 2013年01期 页码: 56-62 栏目: 出版日期: 2013-02-25
Title:
Investigation and Analysis of the Main Agronomic Traits of Different Transgenic Soybean Lines with GsGST14 Gene
文章编号:
1000-9841(2013)01-0056-03
作者:
林凡敏;柏锡;樊超;赵超越;才华;纪巍;朱延明
东北农业大学生命科学学院
Author(s):
LIN Fan-min; BAI Xi; FAN Chao; ZHAO Chao-yue; CAI Hua; JI Wei; ZHU Yan-ming
College of Life Sciences,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
关键词:
转基因大豆; GsGST14基因; 耐盐碱; 农艺性状
Keywords:
Transgenic soybean; GsGST14 gene; Tolerance to saline and alkaline stress; Agronomic traits
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2013.01.013
摘要:
对前期获得的转耐盐碱基因GsGST14的大豆株系HF55-GST14-1、HF55-GST14-2、HF55-GST14-3、HF55-GST14-4、HF55-GST14-5的T3代群体进行抽样PCR阳性检测,结果后代群体中转基因阳性个体比例高于80%,说明对这些后代群体的调查能够反映出GsGST14基因对转基因大豆农艺性状的影响。因此对这5个株系的T3代群体农艺性状进行调查及统计学分析。结果表明,5个转基因株系与对照在单株荚数、单株粒数、百粒重、生育期、结荚习性、花色、叶形和蛋白质含量上无显著差异,转基因株系HF55-GST14-1、HF55-GST14-3与HF55-GST14-4的株高显著低于对照。油分含量HF55-GST14-1显著高于对照,HF55-GST14-2显著低于对照。因此,5个转GsGST14基因大豆株系并未在农艺性状上产生较大的不良变异,具有良好的应用前景。
Abstract:
In the previous study,we have transformed salt tolerance gene GsGST14 into soybean.In the present study, the agronomic traits,including the yield,morphological and quality traits of five transgenic soybean lines,as well as the CK variety Hefeng55,were investigated and analyzed statistically.There were no obvious differences between transgenic soybean and the control in pods per plant,seeds per plant,100-seed weight,growth duration,podding habits,flower color,leaf shape and protein content,but the plant height of HF55-GST14-1，HF55-GST14-3 and HF55-GST14-4 were obviously shorter than control,and the oil content of HF55-GST14-1 and HF55-GST14-2 were significantly higher and lower than that of the receptor variety,respectively.All the results indicated that five transgenic soybean lines with GsGST14 gene had no seriously adverse variation in yield,morphological and quality traits,so they would have good utilization prospects.

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备注/Memo

基金项目：转基因生物新品种培育大专项（2011ZX08004-002）；国家自然科学基金（31171578）；黑龙江省高校科技创新团队建设计划（2011TD055）

第一作者简介：林凡敏（1986-），女，在读硕士，研究方向为植物基因工程。E-mail:mutou11@126.com。
通讯作者：朱延明（1955-），男，教授，博士生导师，从事植物基因工程研究。E-mail:ymzhu2001@neau.edu.cn。

更新日期/Last Update: 2013-02-20