ZHANG Xiu-xiu,WU Hui-jun,ZHOU Yu-cheng,et al.Effect of Xylo-oligosaccharide on Growth and Resistance to SMV in Soybean[J].Soybean Science,2012,31(04):621-624,629.[doi:10.3969/j.issn.1000-9841.2012.04.022]
低聚木糖对大豆生长和花叶病毒病抗性的影响
- Title:
- Effect of Xylo-oligosaccharide on Growth and Resistance to SMV in Soybean
- 文章编号:
- 1000-9841（2012）04-0621-04
- 关键词:
- 低聚木糖; 大豆; 促生; SMV抗性; Quantitative RT-PCR
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 用不同浓度的低聚木糖处理大豆，研究低聚木糖对大豆生长和花叶病毒病（SMV）抗性的影响，温室实验结果表明：低聚木糖溶液处理大豆种子18 d后与对照相比，50 mg·L-1低聚木糖对大豆幼苗的株高、鲜重和主根长有明显的促进作用。低聚木糖喷雾处理大豆叶片18 h后接种SMV，与对照相比显著降低了SMV病情指数。利用Quantitative RT-PCR检测低聚木糖处理的大豆叶片中的防卫相关基因的表达情况，与对照相比，防卫基因PR2、PR10、PR12和LOX2在低聚木糖处理12 h时有最高的相对表达量；PR3在低聚木糖处理18 h时有最高的相对表达量；PR1、PAL、PPO和CHS在低聚木糖处理24 h时显著上调表达。研究表明，低聚木糖激活了大豆SA信号通路和JA信号通路，从而提高了大豆对SMV的抗性。
- Abstract:
- Treated soybean with different concentrations of xylo-oligosaccharide to study the effect of xylo-oligosaccharide on growth and resistance to soybean mosaic virus(SMV)in soybean.The results of greenhouse experiment showed that 18 days after the seed of soybean were dealt with the xylo-oligosaccharide solution,compared with the contol, 50 mg·L-1 xylo-oligosaccharide obviously increased the height,fresh weight,and main root length of soybean seedlings.Inoculated with SMV after 18 hours of pre-atomized the soybean leaves with xylo-oligosaccharide,compared with the contol,the disease index of soybean mosaic virus was obviously reduced.Using Quantitative RT-PCR to test the expression of the defense-related gene of the soybean leaves dealt with xylo-oligosaccharide,compared with the contol,the defense-related gene PR2,PR10,PR12 and LOX2had the highest related expression quantity at the time of 12 hours, PR3 at 18 hours,but PR1,PAL,PPO,and CHS at 24 hours.Results suggest that xylo-oligosaccharide can active salicylic acid(SA)and jasmonic acid(JA)signaling pathways of soybean,and enhances its resistance against SMV.
参考文献/References:
[1]陈利锋,徐敬友.农业植物病理学[M].北京:中国农业出版社,2001:220-222.(Chen L F,Xu J Y.Agricultural phytopathology[M].Beijing:Agricultural Press,2001:220-222.) [2]姬小明,吕全建,赵铭钦.低聚木糖的特性及应用研究进展[J].安徽农业科学,2007,35(23):7088-7089.(Ji X M,LüQ J,Zhao M Q.Research progress of characteristics and application of xylo-oligosaccharide[J].Journal of Anhui Agricultural Sciences,2007,35(23):7088-7089.) [3]贺英.几种低聚糖激发子的制备及其对毛白杨愈伤组织抗病性诱导作用的研究[D].杨凌:西北农林科技大学,2006:30-34.(He Y.Preparation of various oligosaccharides elicitors and research on disease resistance of Populus tomentosa?callus induced by different ellcitors[D].Yangling:Northwest Agriculture and Forestry University,2006:30-34.) [4]Yamaguchi T,Ito Y,Shibuya N.Oligosaccharide elicitors and their receptors for plant defense responses[J].Trends in Glycoscience and Glycotechnology,2000,12(64):113-120. [5]Zhi H J,Gai J Y.Performances and germplasm evaluation of quantitative resistance to soybean mosaic virus in soybeans[J].Agricultural Science in China,2004,3(4):247-253. [6]Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Methods,2001,25(4):402-408. [7]Aguilar F,Montandon P E,Stutz E.Two genes encoding the soybean translation elongation factor eEF-1 alpha are transcribed in seedling leaves[J].Plant Molecular Biology,1991,17:351-360. [8]Kim C S,Yi S Y,Lee Y K,et al.Isolation and differential expression of an acidic PR-1 cDNA gene from soybean hypocotyls infected with Phytophthora sojae?f.sp.glycines[J].The Plant Pathology Journal,2000,16:9-18. [9]Takeuchi Y,Yoshikawa M,Takeba G,et al.Molecular cloning and ethylene induction of mRNA encoding a phytoalexin elicitor-releasing factor,β-1,3-endoglucanase,in soybean[J].Plant Physiology,1990,93:673-682. [10]Gijzen M,Kuflu K,Qutob D,et al.A class I chitinase from soybean seed coat[J].Journal of Experimental Botany,2001,52:2283-2289. [11]Ludwig A,Tenhaken R.Defence gene expression in soybean is linked to the status of the cell death program[J].Plant Molecular Biology,2000,44:209-218. [12]Thomma B P H J,Cammue B P A,Thevissen K.Plant defensins[J].Planta,2002,216:193-202. [13]Frank R L,Vodkin L O.Sequence and structure of a phenylalanine ammonia-lyase gene from Glycine max[J].Mitochondrial DNA,1991,1(5):335-346. [14]Robert G U,Martha E R.Defense-related gene expression in soybean leaves and seeds inoculated with Cercospora kikuchii?and Diaporthe phaseolorum?var.meridionalis[J].Physiological and Molecular Plant Pathology,2010,75:64-70.
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
基金项目：国家转基因生物新品种培育重大专项（2011ZX08004-004）；国家自然科学基金（31100056）；高等学校博士学科点专项科研基金（20100097120011）。