GU Si-yu,LIU Shuang,WANG Jia-jia,et al.Physiological Response of Different Soybeans under Sparingly Soluble Phosphate[J].Soybean Science,2012,31(03):411-415.[doi:10.3969/j.issn.1000-9841.2012.03.015]
不同基因型大豆对难溶性磷胁迫的生理响应
- Title:
- Physiological Response of Different Soybeans under Sparingly Soluble Phosphate
- 文章编号:
- 1000-9841（2012）03-0411-05
- Keywords:
- Soybean; Sparingly soluble phosphate; Adaption mechanism
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 阐明大豆适应难溶性磷胁迫的生理反应，对筛选和培育磷高效基因型大豆工作具有重要意义。采用砂培和水培试验研究了大豆利用难溶性磷源的基因型差异及其生理指标的变化。结果表明：难溶性磷处理下大豆植株磷浓度和含磷量都显著低于高磷处理(P<0.05)，不同基因型大豆的磷浓度和含磷量表现出较大的差异。难溶性磷诱导下，大豆叶片酸性磷酸酶增加；在处理后期，叶片丙二醛（MDA）含量远高于高磷对照；Ca-P和Fe-P处理下，植株磷浓度与叶片酸性磷酸酶呈极显著负相关。
- Abstract:
- Studying the adaptation of soybean plant to low P stress was very important for screening or breeding P-efficient soybean genotypes.A sand culture and a solution culture experiment were conducted to study the genotypic discrepancy and physiology variation of soybean in using insoluble phosphates.The results showed that the P concentration and uptake in insoluble phosphates treatment were lower than that in high phosphates treatment(P<0.05),and there were differences in phosphorus concentration and uptake among different soybean genotypes.The insoluble phosphate induced the increase of acid phosphatase（APA） in leaf and root.At late stage of insoluble phosphates treatment,the malondialdehyde（MDA） content of soybean was higher than that in high phosphates treatment,indicating that P deficiency stress could lead to the accumulation of superoxide free radical and the increase of membrane lipid peroxidation.In Ca-P and Fe-P treatment,the plant P concentration had very significant negative correlation with leaf APA.
参考文献/References:
[1]徐青萍，罗超云，廖红，等 . 大豆不同品种对磷胁迫反应的研究 [J]. 大豆科学， 2003 ， 22(2) ： 108-114.(Xu Q P,Luo C Y,Liao H,et al.Study on the response of soybean varieties to P deficiency[J].Soybean Science ， 2003 ， 22(2) ： 108-114.) [2]李继云，孙建华，刘全友，等.不同小麦品种的根系生理特性、磷的吸收及利用效率对产量影响的研究[J].西北植物学报，2000，20(4)：503-510.(Li J Y,Sun J H,Liu Q Y,Tong Y，et al.A study on the physiological properties of root systems in various wheat varieties and the effects of their phosphorus uptake and utilization efficiency on the yields[J].Acta Botanica Boreali-Occidentalia Sinica,2000,20(4):503-510.) [3]Fageria N K，Baligar V C.Phosphorus use efficiency of wheat genotypes[J].Nutrition，1999，22：331-264. [4]Yan X L，Liao H，Beebe S E，et al.QTL mapping of root hair and acid exudation traits and their relationship to phosphorus uptake in common bean[J].Plant and Soil，2004，265：17-29. [5]Subbarao G V，Ae N T.Genetic variation in acquisition，and utilization of phosphorus from iron-bound phosphorus in pigeonpea[J].Soil Science and Plant Nutrition，1997，43：511-519. [6]Wissuwa M，Ae N.Genotypic variation for phosphorus uptake from hardly soluble iron-phosphate in groundnut(Arachis hypogaesL.)[J].Plant and Soil，1999，206：163-171. [7]Osborne L D，Rengel Z.Genotypic differences in wheat for uptake and utilization of P from iron phosphate[J].Australian Journal of Agricultural Research，2002，53：837-844. [8]Shen H，Yan X L，Zhao M，et al.Exudation of organic acids in common bean as related to mobilization of aluminum-and iron-bound phosphates[J].Environmental and Experimental of Botany，2002，48：1-9. [9]Newrnan E I，Andrews R E.Uptake of phosphorus and potassium in relation to root growth and root density[J].Plant and Soil，1973，38：49-69. [10]Borkert C M，Barber S A.Effect of supplying P to a portion of the soybean root system on root and P uptake kinetics[J].Journal of Plant Nutrition，1983，6：685-910. [11]李海波，夏铭，吴平.低磷胁迫对水稻苗期侧根生长及养分吸收的影响[J].植物学报，2001，43(11)：1154-1160. (Li H B,Xia M,Wu P.Effect of phosphorus deficiency stress on rice lateral root growth and nutrient absorption[J].Acta Botanica Sinica,2001,43(11):1154-1160.) [12]Gahoonia T S，Asmar F，Giese H，et al.Root-released organic acids and phosphorus uptake of two barley cultivars in laboratory and field experiments[J].European Journal of Agronomy，2000，12：281-289. [13]申建波，毛达如.植物营养研究方法[M].北京：〖JP2〗中国农业大学，2011:14-21.(Sheng J B,Mao D R.Plant nutrition research methods[M].Beijing:China Agricultural University,2011:14-21.) [14]鲁如坤.土壤农业化学分析方法[M].北京：中国农业科技出版社，1999：146-188.(Lu R K.Methods in agricultural chemical analysis[M].Beijing:China Agricultural Science and Technology Press,1999：146-188.) [15]McLachlan K D.Acid phosphatase activity of intact roots and phosphorus nutrition in plants.II Variations among wheat roots[J].Australian Journal of Agricultural Research，1980， ?31：429-448. [16]张宪政.作物生理研究法[M].北京:中国农业出版社，1990：15.(Zhang X Z.Research mthod of crop pysiology[M].Beijing:Agricultural Press,1990：15.) [17]Vance C P，LJhde-Stone C，Allan D L.Phosphorus acquisition and use critical adaptations by plants for securing a nonrenewable resource[J].New Phytologist，2003，157：423-447. [18]George C E，Lauchli A.Evaluation of an acid phosphatase assay for detection of phosphorus deficiency in leaves of maize(Zea maysL.)[J].Journal of Plant Nutrition，1986，9：1469-1477. [19]Yun S J，Kaeppler S M.Induction of maize acid phosphatase activities under phosphorus starvation[J].Plant and Soil，2001，237：109-115. [20]McLachlan K D.Effects of drought，aging and phosphorus status on leaf acid phosphatase activity in wheat[J].Australian Journal of Agricultural Research，1984，35：777-787. [21]Yan X L，Liao H.Induction of a major Leaf acid phosphatase does not confer adaptation to low phosphorus availability in common bean[J].Plant Physiology，2001，125：1901-1911 [22]丁洪，李生秀，郭庆元，等.酸性磷酸酶活性与大豆耐低磷能力的相关研究[J].植物营养与肥料学报，1997，3(2)：123-128.(Ding H,Li S X,Guo Q Y,et al.Study on correlation between acid phosphatase activity and low phosphorus tolerance of soybean[J].Plant Nutrition and Fertilizen Science,1997,3(2):123-128.)
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备注/Memo
基金项目：黑龙江省教育厅资助项目（10551033）。