LI Chun-jie,LIU Hai-long,XU Yan-li.Biological Characteristics of Antagonistic Bacteria against Soybean Root Rot[J].Soybean Science,2011,30(05):809-812.[doi:10.11861/j.issn.1000-9841.2011.05.0809]
大豆根腐病拮抗细菌的生物学特性研究
- Title:
- Biological Characteristics of Antagonistic Bacteria against Soybean Root Rot
- 文章编号:
- 1000-9841（2011）05-0809-04
- Keywords:
- Soybean; Root rot; Antagonistic bacteria; Biological characteristics
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以实验室筛选出的对大豆根腐病拮抗效果较好的8株细菌（B01c、B021a、B04b、B06、B072b、B09、B131和B1）为研究对象，对其适宜生长温度、pH、氧气、渗透压、生长速度等生物学特性进行了测定。结果表明：在测试温度中，B01c、B021a、B04b和B131适宜生长温度为（30±1）℃，B06和B09适宜生长温度为（45±1）℃，B1在（30±1）℃和（45±1）℃下均生长较好。8株生防细菌在中性环境中均可较好生长；在pH为3时所有菌株均不能正常生长；除B09外，其它7株细菌在pH为11时均可生长，但较中性环境生长稍差。生防菌株B01c和B09为好氧菌；菌株B021a、B04b、B06和B1为兼性厌氧菌；B072b和B131为耐氧厌氧菌。B04b和B072b为较耐盐型菌株；B131耐盐性较差；其它菌株在正常盐浓度下均可生长。8株生防细菌的生长曲线存在一定的差异。
- Abstract:
- Eight soybean root antagonistic bacteria strains including B01c、B021a、B04b、B06、B072b、B09、B131 and B1 had been screened in precious research,and the biological characteristics of growth temperature,pH,oxygen,osmotic pressure and growth rate of those bacteria were tested in this study.As for the suitable growth temperature,B01c,B021a,B04b and B131 were (30±1)℃,B06 and B09 were (45±1)℃,B1 could grow well at both (30±1)℃ and (45±1)℃.All strains could grow well at pH 7,seven strains could grow at pH 11 except B09,while pH 3 was not suitable for all of them.The growth condition of bacteria in neutral condition was better than pH 11.Among all strains,B01c and B09 were aerobes,B021a,B04b,B06 and B1 were facultative anaerobes,B072b and B131 were aerotolerant anaerobes.B04b and B072b were haloduric,B131 was weak haloduric and other strains could survive at 0.85% and 5% NaCl concentrations.The growth curves showed that 8 biocontrol bacteria had different growth properties.In general,the biological characteristics of 8 antagonistic bacteria strains were different,the research made a foundation for further application of these biocontrol bacteria.
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备注/Memo
基金项目：国家“十一五”科技支撑计划资助项目（2006BAD21B01-15）；黑龙江省“十一五”科技攻关资助项目（GA06B101-1-5）。