LI Yu-qiu,ZHAO Hong-kun,LIU Xiao-dong,et al.Cloning and Phylogenetic Analysis of Mitochondrial Cytochrome b Gene from Soybean(Glycine max)[J].Soybean Science,2011,30(05):738-742.[doi:10.11861/j.issn.1000-9841.2011.05.0738]
大豆线粒体细胞色素b基因的克隆及系统分析
- Title:
- Cloning and Phylogenetic Analysis of Mitochondrial Cytochrome b Gene from Soybean(Glycine max)
- 文章编号:
- 1000-9841（2011）05-0738-05
- Keywords:
- Soybean; Mitochondrial; Cytochromeb
- 分类号:
- Q941
- 文献标志码:
- A
- 摘要:
- 根据实验室测序的大豆线粒体基因组片段设计线粒体细胞色素b(Cytochrome，cob)基因特异引物，PCR扩增获得1 173 bp大豆（JLGM-1B）线粒体cob基因保守区序列。经序列分析，该基因编码391个氨基酸，A+T含量58.1%，G+C含量41.9%。Southern杂交表明cob基因在栽培大豆线粒体基因组中至少有2个拷贝。氨基酸序列及系统进化树分析表明，大豆与其它植物的cob基因具有较高的同源性，与双子叶作物同源性高达98%，其分类地位与传统系统分类地位相吻合，说明cob基因具有较高的保守性，可以作为分子系统分类学潜在的遗传分化标记。
- Abstract:
- In this study，mitochondrial DNA uncompleted sequence was used to design special prime of mitochondrial cytochrome b gene(cob)and obtained conservation domain of soybean cob gene by PCR amplification.The results showed that cob gene was consisted by 1 173 bp which encoded 391 amino acids，with 58.1% A+T and 41.9% G+C content.Southern blot analysis revealed that more than 2 cob gene copies were found in G.max mitochondrial genome.Amino acid sequence and phylogenetic analysis showed that cob was most similar to the dicotyledon，with 98% identity at the nucleotide level.In conclusion，cob gene is a potential hereditary marker for phyletic classification basing on its highly conservative property.
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备注/Memo
基金项目：国家转基因重大专项资助项目(2008ZX08004-004)；国家自然科学青年基金资助项目(31000141)；国家高技术研究发展计划重点资助项目(2006AA10Z120，2011AA10A105)。