YANG Xiao-feng,LU Tao,ZHOU Zheng-jian,et al.Effect of α-Lipoic Acid on the Agrobacterium-tumefaciens Mediated GUS Transient Expression Rate and Shoot Induction Percentage in Soybean(Glycine max L.)[J].Soybean Science,2011,30(04):552-556.[doi:10.11861/j.issn.1000-9841.2011.04.0552]
α-硫辛酸对大豆农杆菌介导GUS瞬时表达和芽诱导的影响
- Title:
- Effect of α-Lipoic Acid on the Agrobacterium-tumefaciens Mediated GUS Transient Expression Rate and Shoot Induction Percentage in Soybean(Glycine max L.)
- 文章编号:
- 1000-9841（2011）04-0552-05
- Keywords:
- Soybean(Glycine max L.); α-Lipoic acid; GUS transient expression; Shoot induction percentage
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以适宜转化大豆受体YC-2为材料，研究了抗氧化剂α-硫辛酸对农杆菌介导GUS瞬时表达和芽诱导率的影响。结果表明：在共培养基中添加不同浓度的抗氧化剂可显著降低外植体的褐化率，随α-硫辛酸浓度的增加外植体褐化率明显减少，但白化率增加，且这些白化的外植体在后期不易产生诱导芽；共培养中添加不同浓度的α-硫辛酸可增强GUS瞬时表达率和芽诱导的再生率，且以25 mg?L-1效果最佳。
- Abstract:
- Antioxidant-α-lipoic acid(LA)influencing on the Agrobacterium-tumefaciens mediated GUS transient expression rate and shoot induction percentage in soybean cultivar‘YC-2’was studied in this paper.The results showed the explant browning percentage was markedly decreased after added different concentrations of α-lipoic acid in the co-cultivation medium.In the meantime,the explant whitening percentage was increased with the increased concentration of α-lipoic acid in co-cultivation medium.Meanwhile,the whitening explant couldn’t induce shoot regeneration after culured on the shoot induction medium for a month.It also showed the transient GUS expression rate and shoot induction frequency were also increased after added different LA concentrations in the co-cultivation medium.The optimized LA concentration was 25 mg?L-1in the co-cultivation medium.
参考文献/References:
[1]Wojtaszek P.Oxidative burst:an early plant response to pathogen infection[J].Biochemistry Journal,1997，322:681-691. [2]Dan Y.Lipoic acid-a unique plant transformation enhancer[J].In Vitro Cell Development Biology-Plant,2009,45:630-638. [3]Enríquez-Obregón G A,Prieto-Samsónov D L,Riva G A,et al.Agrobacterium-mediated japonica rice transformation:a procedure assisted by an antinecrotic treatment[J].Plant Cell Tissue Organ Culture,1999,59(3):159-168. [4]Olhoft P M,Flagel C M,Denovan,et al.Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method[J].Plant,2003,216:723-735. [5]Olhoft P M,Bernal L B G,Hill S L,et al.A novel Agrobacterium rhizogenes?mediated transformation method of soybean[Glycine max?(L.)Merrill] using primary-node explants from seedlings[J].In Vitro Cell Development Biology-Plant,2007,43(6):536-549. [6]Zheng Q S,Ju B,Liang L K,et al.Effects of antioxidants on the plant regeneration and?GUS?expressive requency of peanut(Arachis hypogaea) explants byAgrobacterium?tumefaciens[J].Plant Cell Tissue Organ Culture,2005,811:83-89. [7]Packer L,Tritschler H.Alpha-lipoic acid:the metabolic antioxidant[J].Free Radical Biology-Medicine,1996,20:625-626. [8]Packer L,Tritschler H,Wessel K.Neuro protection by the metabolic antioxidant alpha-lipoic acid[J].Free Radical Biology-Medicine,1997,22:359-378. [9]Halliwell B.How to characterize a biological anti-oxidant[J].Free Radical Research Communication,1990,9:1-32. [10]Halliwell B.Antioxidants in human health and disease[J].Annual Review Nutrition,1996,16:33-50. [11]Dan Y H,Armstrong C L,Dong J,et al.Lipoic acid-a unique plant transformation enhancer[J].In Virto Cell Development Biology-Plant,2009,45(6):630-638. [12]Zhang Z Y，Xing A Q，Staswick P.The use of glufosinate as a selective agent in?Agrobacterium-mediated transformation of soybean[J].Plant Cell Tissue and Organ Culture,1999,56:37-46. [13]Jefferson R A.Assaying chimeric genes in plants:the GUS?gene fusion system[J].Plant Molecular Biology Reporter,1987,5:387-405. [14]Perl A,Lotan O,Abu-Abied M,et al.Establishment of an Agrobacterium-mediated transformation system for grape(Vitis vinifera?L.):the role of antioxidants during grape-Agrobacterium?interactions[J].Nature Biotechnology,1996,14:624-628. [15]Kuta D D,Kuta L,Tripathi.Agrobacterium-induced hypersensitive necrotic reaction in plant cells:a resistance response against Agrobacterium-mediated DNA transfer[J].African Journal of Biotechnology,2005,4(8):752-757. [16]Han M L,Gleave A P，Wang T C.Efficient transformation of Actinidia arguta by reducing the strength of basal salts in the medium to alleviate callus browning[J].Plant Biotechnology Reporter,2010,4:129-138.
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
收稿日期：2011-05-04基金项目：国家转基因专项资助项目（2009ZX08010-013B，2008ZX08004-0004，2008ZX08004-001）；国家自然科学基金资助项目（31071443）。