AN Hong-bo,QIN Xue-gong,CHEN Xiang,et al.Research on Deactivated Methods of Soybean Urease Anti-nutrition Factors[J].Soybean Science,2011,30(02):294-297.[doi:10.11861/j.issn.1000-9841.2011.02.0294]
大豆中脲酶抗营养因子的钝化方法研究
- Title:
- Research on Deactivated Methods of Soybean Urease Anti-nutrition Factors
- 文章编号:
- 1000-9841（2011）02-0294-04
- Keywords:
- Soybean; Urease; Anti-nutritional factors; Deactivated methods
- 分类号:
- S816.42
- 文献标志码:
- A
- 摘要:
- 采用正交试验设计对化学钝化法(亚硫酸钠法)和物理钝化法（微波法和超声波法）进行比较。结果表明：化学法钝化的最优钝化工艺条件为：Na2SO3 0.03 mol·L-1，温度75℃，钝化时间60 min；物理微波法钝化为：微波功率230 W，时间90 s，粉碎度为过60目筛；物理超声波钝化法为：加水量20 mL，时间30 min，粉碎度为过60目筛。3种钝化方法处理后，脲酶活性均低于0.3 U，符合优质饲料的要求。
- Abstract:
- The present study compared the chemical and physical deactivation methods of urease anti-nutrition factors in soybean meal by orthogonal experimental design. The results showed that the optimized conditions were deactivated soybean meal with 0.03 mol·L-1 Na2SO3 for 60 min at 75℃ for chemical deactivation method; treated at 230 W for 90 s with the soybean particle size of 60 mesh sieve for microwave deactivation method;adding 20 mL H2O reacted 30 min with the soybean particle size of 60 mesh sieve for physical ultrasonic method. The urease activities were lower than 0.3 U after these three deactivated processes and accorded with the standard of high quality feeds.
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备注/Memo
基金项目：黑龙江省农垦总局科技计划资助项目（HNKXIV-02-07a）。