WEI Lai,KOU Kun,TANG Xiao-fei,et al.Evaluation of Genetic Diversity of Soybean Introductions and Chinese Accessions Using SSR Markers[J].Soybean Science,2011,30(02):184-189,193.[doi:10.11861/j.issn.1000-9841.2011.02.0184]
中国与引进国外大豆种质资源遗传多样性分析
- Title:
- Evaluation of Genetic Diversity of Soybean Introductions and Chinese Accessions Using SSR Markers
- 文章编号:
- 1000-9841（2011）02-0184-06
- Keywords:
- Soybean; Diversity; Allelic variation
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 利用11对SSR引物分析了来自中国、加拿大、美国、俄罗斯和意大利的共166份栽培大豆种质资源，共检测到33个等位变异，平均每对引物检测到的等位变异数为3.09，遗传多样性指数为0.946。中国栽培大豆种质具有较丰富的遗传多样性，聚类分析结果表明中国和俄罗斯大豆相似系数较大，初步认定中俄大豆的亲缘关系较近。加拿大和意大利的材料相似系数较高，推测两国间某些材料有相似的遗传背景。中国与美国大豆品种间遗传差异较大，可以进行种质资源的交换利用。
- Abstract:
- For extending soybean genetic basis and overcoming the narrow genetic basis of choosing parents when creating a new cross combination, we used PCR technique to find the allelic variation among soybean germplasm resources of different countries. SSR(Simple Sequences Repeats) variation was assessed in 166 sample of soybean from China, Canada, United States, Russia and Italy with 11paired-primers. In total we found 33 locus, averaging 3.09 per locus, and the average polymorphism information content (PIC) was 0.946. We believed that Chinese soybean had more polymorphism than other countries, and China and Russia have adjacent relationship. The samples from Canada and Italy had high similarity coefficient, so we supposed that they could have similar genetic background. The samples from China and that from United States had a huge differences in genetic diversity.
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备注/Memo
基金项目：国家高技术研究发展计划（863计划）重点资助项目（20065AA102F9）；大豆产业技术体系资助项目； 引进国际先进农业科学技术计划（948计划）资助项目（2006-G5）。