[1]王 林,韩 飞,李爱科,等.不同加工工艺对大豆转基因成分及调控元件的影响[J].大豆科学,2011,30(01):136-140.[doi:10.11861/j.issn.1000-9841.2011.01.0136]
　WANG Lin,HAN Fei,LI Ai-ke,et al.Effect of Different Processing on Transgenic Ingredients and Regulatory Elements of Soybean[J].Soybean Science,2011,30(01):136-140.[doi:10.11861/j.issn.1000-9841.2011.01.0136]
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不同加工工艺对大豆转基因成分及调控元件的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第30卷 期数: 2011年01期 页码: 136-140 栏目: 出版日期: 2011-02-25
Title:
Effect of Different Processing on Transgenic Ingredients and Regulatory Elements of Soybean
文章编号:
1000-9841（2011）01-0136-05
作者:
王 林1; 2; 韩 飞1; 李爱科1; 刘建学2; 王瑛瑶1
1．国家粮食局 科学研究院，北京 100037；
2．河南科技大学 食品学院，河南 洛阳 471003
Author(s):
WANG Lin1; 2; HAN Fei1; LI Ai-ke1; LIU Jian-xue2; WANG Ying-yao1
1. Academy of State Administration of Grain，Beijing 100037；
2. Institute of Food，Henan University of Science and Technology，Luoyang 471003，Henan, China
关键词:
大豆; 加工工艺; 内源基因; 外源基因; 启动子; 终止子; PCR技术
Keywords:
Soybean; Processing; Endogenous gene; Exogenous gene; Promoter; Terminator; PCR technology
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2011.01.0136
文献标志码:
A
摘要:
以转基因大豆为试验材料，运用定性PCR的方法研究了粉碎、湿热和膨化3种加工工艺对转基因大豆内源基因Lectin、外源基因Cp4 epsps以及启动子和终止子的影响。结果表明：大豆经过100℃湿热处理15 min后，内源基因降解到407 bp以下；经过100℃湿热处理15 min后，外源基因降解到408 bp以下；在100℃经3 min湿热处理后启动子和终止子都产生了降解。机械粉碎与液氮粉碎都未能使大豆基因产生降解，而膨化工艺使大豆内源基因降解到836 bp以下，外源基因降解到1 512 bp以下，但启动子与终止子并未产生降解。因此，粉碎、湿热和膨化加工工艺对大豆转基因成分及调控元件的降解都有不同程度的影响。
Abstract:
Based on generally modified soybeans as materials，used the qualitative research methods of PCR to study the effects to Lectin gene and exogenous gene of Cp4 epspsand promoter and terminator by crushing and sticky-heating and extrusion processing. Researches showed that endogenous gene degraded below to 407 bp through processing at 100℃ sticky-heating for 15 min. Exogenous gene degraded below to 408 bp after 100℃ sticky-heating for 15 min. Promoter and terminator turn to degrade by 100℃ sticky-heating for 3 min. The results also indicated that gene sections had no changes through mechanical disintegration and liquid nitrogen processings. While promoter and terminator showd no degrades through extrusion processing, on the contrary, endogenous gene degraded below to 836 bp and exogenous gene degraded below to 1 512 bp .

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备注/Memo

基金项目：转基因生物新品种培育重大专项资助项目（2009ZX08012-012B）。

第一作者简介：王林（1985-），男，在读硕士，主要从事生物技术研究。E-mail：wangruilinwang@yahoo.com.cn。
通讯作者：韩飞（1973-），男，副研究员，博士，主要从事粮食（食品）营养与安全研究。E-mail：hf@chinagrain.org。

更新日期/Last Update: 2014-09-11