[1]王婵婵,王安娜,吴蕾,等.大豆PAL2基因的克隆与分析[J].大豆科学,2010,29(01):13-17.[doi:10.11861/j.issn.1000-9841.2010.01.0013]
　WANG Chan-chan,WANG An-na,WU Lei,et al.Cloning and Analysis of PAL2 Gene from Soybean[J].Soybean Science,2010,29(01):13-17.[doi:10.11861/j.issn.1000-9841.2010.01.0013]
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大豆PAL2基因的克隆与分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年01期 页码: 13-17 栏目: 出版日期: 2010-02-25
Title:
Cloning and Analysis of PAL2 Gene from Soybean
文章编号:
1000-9841（2010）01-0013-05
作者:
王婵婵; 王安娜; 吴蕾; 李业成; 刘成; 马凤鸣
东北农业大学 农学院 ，黑龙江 哈尔滨 150030
Author(s):
WANG Chan-chan; WANG An-na; WU Lei; LI Ye-cheng; LIU Cheng; MA Feng-ming
College of Agriculture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
关键词:
大豆; 苯丙氨酸解氨酶（PAL）; 序列分析
Keywords:
Soybean Phenylalanine Ammonia-lyase(PAL); Sequence analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.01.0013
文献标志码:
A
摘要:
采用RT-PCR法克隆了1个大豆PAL基因，并利用网络工具分析预测了其编码蛋白。结果表明：该基因是大豆PAL基因家族的1个新成员，命名为GmPAL2（登录号：GQ220305），其全长2284bp，编码1个包含717个氨基酸残基的多肽链，分子量为78.116kD；经BLASTp比对表明：该蛋白序列与菜豆PAL2蛋白同源性最高，达到92%，与大豆PAL1的同源性为89%。该研究结果为进一步研究整个大豆PAL基因家族的特性奠定了基础。
Abstract:
The PAL2 gene，cloned from soybean by RT-PCR，is a new member of PAL gene family.It is named GmPAL2 and the accession number is GQ220305.The protein encoded by PAL2 was analyzed by informatics tools provided on internet.The complete cDNA of PAL2 was 2284bp and it encoded a polypeptide chain which contained 717 amino acid residues.The molecular weight of the protein encoded by PAL2 gene was 78.116kD.The homology of soybean PAL2 and bean PAL2 reached 92% by BLASTp tool，the homology of soybean PAL2 and soybean PAL1 was only 89%.The olotained PAL2 gene will be a base for the recombinant expression and the analysis of PAL gene family in soybean.

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备注/Memo

基金项目：黑龙江省自然科学基金重点资助项目(ZJN0701)。

第一作者简介：王婵婵(1983-),女,硕士,研究方向为作物生理与分子生物学。E-mail:wccttss@163.com。
通讯作者：马凤鸣，教授，博士生导师。E-mail: fengming_ma@sohu.com。

更新日期/Last Update: 2014-09-12