[1]刘众悦,王红玲,刘春,等.中国大豆种质11S球蛋白A5A4B3和A3B4亚基缺失的分子机制[J].大豆科学,2009,28(03):363-369.[doi:10.11861/j.issn.1000-9841.2009.03.0363]
　LIU Zhong-yue,WANG Hong-ling,LIU Chun,et al.Molecular Mechanism of A5A4B3null and A3B4null of Chinese Soybean Germplasm[J].Soybean Science,2009,28(03):363-369.[doi:10.11861/j.issn.1000-9841.2009.03.0363]
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中国大豆种质11S球蛋白A5A4B3和A3B4亚基缺失的分子机制

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年03期 页码: 363-369 栏目: 出版日期: 2009-06-25
Title:
Molecular Mechanism of A5A4B3null and A3B4null of Chinese Soybean Germplasm
文章编号:
1000-9841(2009)03-0363-07
作者:
刘众悦1; 王红玲1; 2; 刘春1; 3; 麻浩1
1南京农业大学作物遗传与种质创新国家重点实验室，南京农业大学大豆所，江苏 南京 210095；
2江苏省林业科学院遗传育种研究所，江苏 南京 211153；
3衡阳师范学院生命科学系，湖南 衡阳 421008
Author(s):
LIU Zhong-yue1; WANG Hong-ling1; 2; LIU Chun1; 3; MA Hao1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement，Soybean Research Institue，Nanjing Agricultural University，Nanjing 210095,Jiangsu；
2Institute of Genetics and Breeding，Forest Academy of Jiangsu Province，Nanjing 211153,Jiangsu；
3Department of Life Science，Hengyang Normal University，Hengyang 421008,Hunan,China
关键词:
大豆; A5A4B3亚基; A3B4亚基; 亚基缺失; 密码子点突变
Keywords:
Soybean; A5A4B3subunit; A3B4?subunit; Subunit lacking; Point mutation of codon
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.03.0363
文献标志码:
A
摘要:
我国大豆种质资源中存在着丰富的主要贮藏蛋白亚基变异类型，它们是大豆品质改良和育种重要的种质基础，因而研究其变异发生的机制有着积极的指导作用。以7个A5A4B3亚基缺失体、2个A3B4亚基缺失体和正常品种为材料，在采用SDS-PAGE验证亚基缺失表现稳定的前提下，克隆得到缺失亚基所对应的基因序列和cDNA序列，然后通过与NCBI上已公布的正常序列进行比较，发现7个材料编码A5A4B3亚基的DNA序列和cDNA序列的起始密码子都由ATG突变成了ATA，形成一个严重错误的翻译阅读框，引起缺失；2个材料编码A3B4亚基的DNA序列并无明显差异，但cDNA序列的终止密码子都由TAA突变成了CAA，可能会导致翻译出来的亚基前体额外多出17个氨基酸的尾巴，引起缺失。
Abstract:
There is great genetic diversity in the relative content of seed storage protein subunits in Chinese soybean germplasm，which is the foundation of soybean protein quality improvement.Therefore，it will be helpful for soybean high-quality protein breeding to understand the molecular mechanism of the subunit mutations.Based on the validation of their subunit deficiency by sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE)，seven landraces without A5A4B3and two without A3B4subunit were used as experimental materials and their subunit mutant gene sequences and cDNA sequences were obtained.Compared with normal sequences on NCBI，the start codon ATG of the genes and cDNAs encoding the A5A4B3subunits of seven mutant landraces was found to mutate to ATA，which produced a fire-new reading frame of translation and resulted in subunit lacking.While the stop codon TAA of cDNAs encoding A3B4subunit of two mutant landraces were found to mutate to CAA，which may resulted in an additional tail in pro-glycinin and caused subunit lacking.

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备注/Memo

基金项目：引进国际先进农业计划（948计划）资助项目(2001-207)；高等学校学科创新引智计划资助项目。

作者简介：刘众悦(1985-)，男，硕士研究生，现主要从事大豆品质改良与利用研究。王红玲为共同第一作者。
通讯作者：麻浩，教授，博士生导师。E-mail：Lq-ncsi@njau.edu.cn。

更新日期/Last Update: 2014-09-25