[1]郑树贵,董维国,孙泽威,等.等电点沉淀法分离大豆球蛋白酸性亚基和碱性亚基[J].大豆科学,2009,28(01):136-139.[doi:10.11861/j.issn.1000-9841.2009.01.0136]
　ZHENG Shu-gui,DONG Wei-guo,SUN Ze-wei,et al.Isolation of Acidic Subunits and Basic Subunits from Glycinin using Isoelectric Point Precipitation[J].Soybean Science,2009,28(01):136-139.[doi:10.11861/j.issn.1000-9841.2009.01.0136]
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等电点沉淀法分离大豆球蛋白酸性亚基和碱性亚基

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年01期 页码: 136-139 栏目: 出版日期: 2009-02-25
Title:
Isolation of Acidic Subunits and Basic Subunits from Glycinin using Isoelectric Point Precipitation
文章编号:
1000-9841(2009)01-0136-04
作者:
郑树贵1; 2; 董维国2; 孙泽威1; 秦贵信1
1吉林农业大学动物科技学院，吉林 长春 130118；
2 沈阳农业大学畜牧兽医学院，辽宁 沈阳 110161
Author(s):
ZHENG Shu-gui1; 2; DONG Wei-guo1; SUN Ze-wei1; QIN Gui-xin1
1College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，Jilin；
2College of Animal Husbandry and Veterinary Medicine，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
分离; 酸性亚基; 碱性亚基; 纯度; 产率
Keywords:
Isolation; Acidic subunits; Basic subunits; Purity; Productive rate
分类号:
TS214
DOI:
10.11861/j.issn.1000-9841.2009.01.0136
文献标志码:
A
摘要:
利用β-巯基乙醇切断大豆球蛋白酸性亚基和碱性亚基之间二硫键的基础上，根据两类亚基等电点的差异分离纯化大豆球蛋白亚基，从而建立了分离大豆球蛋白酸性亚基和碱性亚基的等电点沉淀法。利用这一方法分离得到的酸性亚基和碱性亚基纯度分别为92.69%和80.76%，酸性亚基和碱性亚基的净产率分别达31.40%和41.64%。该方法是一种简单有效、价格低廉的大豆球蛋白亚基分离方法。
Abstract:
A novel isoelectric point precipitation method of isolation of Glycinin acidic and basic subunit was developed.In this approach，two kinds of subunits was isolated according to the difference of isoelectric point of the subunits after disulfide bonds between acidic and basic subunits was cut off using β-mercaptoethanol.The purity of acidic and basic subunits prepared by this approach were 92.69% and 80.76%，and productive rate reached 31.40%，41.64%，repectively.So the approach we developed was a simple，efficient and cheap method for isolation of Glycinin acidic and basic subunits.

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备注/Memo

基金项目：国家自然科学基金重点项目(30430520)。

作者简介：郑树贵(1972-)，男，讲师，现从事大豆抗营养因子研究。E-mail:zhengshugui1001@163.com。

更新日期/Last Update: 2014-10-03