[1]顾地周,车喜全,朱俊义,等.独角莲不同提取液对大豆蚜虫的生物活性及活性浓度的筛选[J].大豆科学,2008,27(06):1010-1014.[doi:10.11861/j.issn.1000-9841.2008.06.1010]
　GU Di-zhou,CHE Xi-quan,ZHU Jun-yi,et al.Biological Activity Against Aphis Glycines of Various Extraction from Typhonium giganteum Engler and Preliminary Screening of Activitive Concentration[J].Soybean Science,2008,27(06):1010-1014.[doi:10.11861/j.issn.1000-9841.2008.06.1010]
点击复制

独角莲不同提取液对大豆蚜虫的生物活性及活性浓度的筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年06期 页码: 1010-1014 栏目: 出版日期: 2008-12-25
Title:
Biological Activity Against Aphis Glycines of Various Extraction from Typhonium giganteum Engler and Preliminary Screening of Activitive Concentration
文章编号:
1000-9841(2008)06-1010-05
作者:
顾地周1; 车喜全2; 朱俊义1; 何晓燕1; 冯颖1; 姜云天1
1 通化师范学院生物系，吉林 通化134002；
2 通化师范学院化学系，吉林 通化134002
Author(s):
GU Di-zhou1; CHE Xi-quan2; ZHU Jun-yi1; HE Xiao-yan1; FENG Ying1; JIANG Yun-tian1
1 Department of Biology，Tonghua Normal University，Tonghua 134002 Jilin；
2 Department of Chemistry，Tonghua Normal University，Tonghua 134002，Jilin，China
关键词:
独角莲; 大豆蚜虫; 均匀设计; 生物活性
Keywords:
Typhonium giganteum Engler; Aphis glycines; Uniform Design; Biological activity
分类号:
S435.6
DOI:
10.11861/j.issn.1000-9841.2008.06.1010
文献标志码:
A
摘要:
基于均匀设计法研究了独角莲球根茎的不同提取液对大豆蚜虫的触杀和拒食作用。结果表明：乙醇浸提液对大豆蚜虫具有较高的触杀活性。水蒸汽蒸馏液对大豆蚜虫具有较强的拒食活性。乙醇浸提液对大豆蚜虫48 h的致死中浓度(LC50)为8.71 mg.mL-1，水蒸汽蒸馏液对大豆蚜虫24、48 h的拒食中浓度(AFC50)分别为8.66、7.93 mg.mL-1。乙醚浸提液对大豆蚜虫的触杀和拒食作用不显著。60 h的同时触杀和拒食活性浓度筛选的结果表明，乙醇浸提液24.1545 mg.mL-1和水蒸汽蒸馏液23.014 mg.mL-1联合使用对大豆蚜虫同时触杀和拒食活性最好，死亡率达100%。
Abstract:
Contacting death and antifeeding against Aphis glycines of various extraction from the bulbs of Typhonium giganteum Engler were studied through Uniform Design.The results showed that the 48 h LC50of death was 8.71 mg.mL-1of ethanol extract，the 24 h and 48 h AFC50of antifeedant were 8.66，7.93 mg.mL-1of distillation liquid.The results of contacting death and antifeedant against Aphis glycines showed that 24.1545 mg.mL-1of ethanol extract and 23.014 mg.mL-1of wet distillation liquid had simultaneously the best contacting death and antifeedant activity，the 60 h rate of death was 100%.As a result，ethanol extract had high contacting death activity，distillation liquid had strong antifeedant activity，ethylether extracts had indistinctively contacting death and antifeedant effect.

参考文献/References:

[1]方开泰.均匀设计—数论方法在试验设计的应用[J].应用数学学报，1980，3(4):363-372.(Fang K T.Appication of Uniform Design-number theretic method in trial design[J].Acta Mathematicae Applicatae Sinica，1980，3(4):363-372.)

[2]王万能，全学军，陆天健.均匀设计超声波提取豆粕异黄酮的研究[J].生物数学学报，2007，22(1)：153-156.（Wang W N，Quan X J，Lu T J.Research on the extraction of isoflavones from soy residue with Uniform Design[J].Journal of Biomathematics，2007，22(1)：153-156.）
[3] Kim S I,Roh J Y,Kim D H,et al.Insecticidal activities of aromatic plant extracts and essential oils against Sitophilus oryzae and Callosobruchus chinensis[J].Journal of Stored Products Research，2003，39：293-303.
[4] Broadway R M，Duffey S S.Plant proteinase inhibitors：Mechanism of action and effect on the growth and digestive physiology of Larval Heliothis zea and Spodoptera exiqua[J].Insect Physiology，1986，32(10)：827-833.
[5] Klock J A，Hum Y，Chiu S F.Grayanoid diterpene insect antifeedants and insecticides from Rhododendron molle[J].Phytochemistry，1991，30(6)：1797-1800.
[6] Miyazawa M，Tsukamoto T，Anzai J，et al.Insecticidal effect of phthalides and furanocoumarins from Angelica acutiloba against Drosophila melanogaster[J].Journal of Agricultural and Food Chemistry，2004，52(14)：4401-4405
[7] Wu W J，Wang M A，Zhu J B，et al.Five new insecticidal sesquiterpenoid from Celastrus angulatus[J].Journal of Natural Products，2001，64(3)：364-367.

相似文献/References:

[1]张俊杰,王瑞珍,肖 亮,等.大豆蚜虫生物型鉴定初探[J].大豆科学,2013,32(02):234.[doi:10.3969/j.issn.1000-9841.2013.02.021]
　ZHANG Jun-jie,WANG Rui-zhen,XIAO Liang,et al.Primary Identification of Soybean Aphid Biotypes[J].Soybean Science,2013,32(06):234.[doi:10.3969/j.issn.1000-9841.2013.02.021]
[2]杨晓贺.佳木斯地区大豆蚜及其天敌种群发生规律的研究[J].大豆科学,2014,33(01):95.[doi:10.11861/j.issn.1000-9841.2014.01.0095]
　YANG Xiao-he.Population Occurrence of Soybean Aphid and Its Natural Enemies in Jiamusi Area[J].Soybean Science,2014,33(06):95.[doi:10.11861/j.issn.1000-9841.2014.01.0095]
[3]赵云彤,时新瑞,孟祥海,等.牡丹江丘陵半山区利用苦参碱、烟碱+皂素防治大豆蚜虫效果研究[J].大豆科学,2014,33(02):287.[doi:10.11861/j.issn.1000-9841.2014.02.0287]
　ZHAO Yun-tong,SHI Xin-rui,MENG Xiang-hai,et al.Biological Control Effect of Matrine and Nicotine+Saponin on Aphis glycines in Mudanjiang Hilly Midlevels[J].Soybean Science,2014,33(06):287.[doi:10.11861/j.issn.1000-9841.2014.02.0287]
[4]杨晓贺,张瑜,丁俊杰,等.三江平原地区大豆蚜虫及其天敌种群发生规律的研究[J].大豆科学,2011,30(04):700.[doi:10.11861/j.issn.1000-9841.2011.04.0700]
　YANG Xiao-he,ZHANG Yu,DING Jun-jie,et al.Population Occurrence of Soybean Aphid and Its Natural Enemies in Sanjiang Plain Area[J].Soybean Science,2011,30(06):700.[doi:10.11861/j.issn.1000-9841.2011.04.0700]
[5]孟凡立,刘立承,李文滨,等.蚜虫取食和机械损伤对大豆真叶中异黄酮的诱导作用[J].大豆科学,2010,29(02):264.[doi:10.11861/j.issn.1000-9841.2010.02.0264]
　MENG Fan-li,LIU Li-cheng,LI Wen-bin,et al.Comparing on the Inducing Isoflavonoids Content in Leaf of Soybean Aroused by Soybean Aphid and Mechanical Damage[J].Soybean Science,2010,29(06):264.[doi:10.11861/j.issn.1000-9841.2010.02.0264]
[6]李娜,于希森,李琪,等.大豆异黄酮合成关键酶基因对蚜虫取食的防御响应分析[J].大豆科学,2016,35(05):800.[doi:10.11861/j.issn.1000-9841.2016.05.0800]
　LI Na,YU Xi-sen,LI Qi,et al.The Analysis of Key Gene of Soybean Isoflavone Synthesis Response to Aphid Feeding Defense[J].Soybean Science,2016,35(06):800.[doi:10.11861/j.issn.1000-9841.2016.05.0800]
[7]季宏平.几种新药剂防治大豆害虫效果及其评价[J].大豆科学,2002,21(01):78.[doi:10.11861/j.issn.1000-9841.2002.01.0078]
　Ji Hongping.EFFECTOF SEVERAL INSECTICIDES ON CONTROLLINGSOYBEAN PESTS AND EVALUATION[J].Soybean Science,2002,21(06):78.[doi:10.11861/j.issn.1000-9841.2002.01.0078]
[8]王素云,暴祥致,孙雅杰,等.大豆蚜虫对大豆生长和产量影响的试验[J].大豆科学,1996,15(03):243.[doi:10.11861/j.issn.1000-9841.1996.03.0243]
　[J].Soybean Science,1996,15(06):243.[doi:10.11861/j.issn.1000-9841.1996.03.0243]
[9]孙志强,田佩占,王继安.野生大豆抗蚜性的利用研究 Ⅰ 栽培大豆和野生大豆杂交F2代的抗蚜性[J].大豆科学,1991,10(02):998.[doi:10.11861/j.issn.1000-9841.1991.02.0098]
　[J].Soybean Science,1991,10(06):998.[doi:10.11861/j.issn.1000-9841.1991.02.0098]

备注/Memo

基金项目：吉林省教育厅自然科学基金资助项目［(2003)-65］。

作者简介：顾地周(1973-)，男，讲师，主要从事长白山区珍稀濒危植物和药用植物研究。E-mail:gudizhou@163.com。

更新日期/Last Update: 2014-10-06