[1]张达,郝再彬,王豫颖,等.矮化大豆突变体茎、叶的解剖学研究[J].大豆科学,2008,27(04):599-603.[doi:10.11861/j.issn.1000-9841.2008.04.0599]
　ZHANG Da,HAO Zai-bin,WANG Yu-ying,et al.Anatomy Structures of Leaves and Stems in the Dwarfing Mutant Soybean[J].Soybean Science,2008,27(04):599-603.[doi:10.11861/j.issn.1000-9841.2008.04.0599]
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矮化大豆突变体茎、叶的解剖学研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年04期 页码: 599-603 栏目: 出版日期: 2008-08-25
Title:
Anatomy Structures of Leaves and Stems in the Dwarfing Mutant Soybean
文章编号:
1000-9841(2008)04-0599-05
作者:
张达; 郝再彬; 王豫颖; 苍晶
东北农业大学生命科学学院，黑龙江 哈尔滨 150030
Author(s):
ZHANG Da; HAO Zai-bin; WANG Yu-ying; CANG Jing
Life Science College of Northeast Agricultural University，Harbin 150030，Heilongjiang，China
关键词:
大豆; 矮化; 解剖; 茎; 叶
Keywords:
Soybean; Dwarf; Anatomic Structure; Stem; Leaf
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0599
文献标志码:
A
摘要:
用NaN3处理大豆品种东农42获得了矮化突变体东泽11号，以东农42为野生型和该突变体为材料，采用石蜡切片法对野生型大豆东农42及其矮化突变体进行了解剖结构研究。结果表明：突变体茎薄壁细胞的横向长度（宽）小于野生型，薄壁细胞的纵向长度（长）大于野生型，单位面积薄壁细胞数目突变体小于野生型，但差异不显著。叶片厚度、栅栏组织厚度、海绵组织厚度、海绵组织占叶片厚度突变体均小于野生型，栅栏组织占叶片厚度、栅/海比突变体大于野生型，且随着矮化程度的增加而增大。突变体的叶主脉维管束个数、导管个数、导管直径、输水面积、韧皮部面积均小于野生型。栅/海可作为矮化指标的筛选。
Abstract:
Dwarf，a excellent agronomic index，is thought to play important roles in improving the growing density and enhancing the efficiency of photosynthesis.The soybean dwarf mutant Dongze 11 was obtained from Dongnong 42 treated with NaN3.Anatomic structures of leaves and stem were observed by paraffin-slice-up in mutant soybean and wild type.The results indicated that shorter transverse length of parenchyma cell (width)，longer vertical length and smaller parenchyma cell number per unit area (no significant difference) were detected in dwarf mutant.Morphological measurements revealed that blade-thickness，palisade tissue-thickness，spongy-thickness and proportion of spongy-thickness and palisade tissue-thickness were all smaller in the dawarf mutant plants，and those characters was notably different in shorter dawarf plants.The numbers of main vascular bundle，vessel number，vessel diameter，area of transporting water and area of phloem in main vein of leaves were lower in the dawarf mutant soybean.In the present study，the dwarfing seedlings were screened，based on tissue propotion of spongy thickness and palisade thickness.

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备注/Memo

基金项目：国家高技术研究发展计划“863”计划资助项目（2006AA100104）；黑龙江省自然科学基金资助项目（AC2005-32）。

作者简介：张达（1977-），女，讲师，博士，主要从事植物生理生化研究。E-mail：zhangda77_@163.com。

更新日期/Last Update: 2014-10-07