[1]张达,王军虹,王豫颖,等.矮化大豆突变体的GA 3调控[J].大豆科学,2008,27(03):456-460.[doi:10.11861/j.issn.1000-9841.2008.03.0456]
　ZHANG Da,WANG Jun-hong,WANG Yu-ying,et al.GA3?Regulation in the Dwarfing Mutant Soybean[J].Soybean Science,2008,27(03):456-460.[doi:10.11861/j.issn.1000-9841.2008.03.0456]
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矮化大豆突变体的GA 3调控

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 456-460 栏目: 出版日期: 2008-06-25
Title:
GA3?Regulation in the Dwarfing Mutant Soybean
文章编号:
1000-9841(2008)03-0456-05
作者:
张达; 王军虹; 王豫颖; 苍晶; 郝再彬
东北农业大学生命科学学院，黑龙江 哈尔滨 150030
Author(s):
ZHANG Da; WANG Jun-hong; WANG Yu-ying; CANG Jing; HAO Zai-bin
Life Science College of Northeast Agricultural University，Harbin 150030，Heilongjiang，China
关键词:
大豆; 矮化突变体; GA3; ELISA
Keywords:
Soybean; Dwarf mutant; GA3; ELISA
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0456
文献标志码:
A
摘要:
矮化是一种优良的农艺性状，因其可改善种植密度，提高光能利用率，近些年引起广泛重视。用NaN3处理大豆品种“东农42”获得了矮化突变体“东泽11号”，以“东农42”为野生型和该突变体为材料，从外源激素处理和内源激素含量测定两方面探讨了GA3对矮化突变体的调控，期望为大豆矮化机理的揭示提供依据。结果表明：外源喷施GA3可明显促进突变体茎伸长，且随着处理时间的延长，GA3对突变体的节间伸长影响愈来愈明显；不同浓度相比，0.60 mmol.L-1GA3促长作用最显著。外源GA3对突变体幼茎切段的促长作用最显著的浓度为2.9×10-4mmol.L-1，表明突变体内源GA3含量低，且12 h处理最显著。ELISA检测植物体内GA3含量显示，植物体内GA3含量不同生育期都表现出突变体小于野生型，表明突变体节间缩短可能与其体内GA3含量低有关。
Abstract:
Dwarf，an excellent agronomic character，was thought to play important roles in improving the growing density and enhancing the photosynthesis efficiency.The dwarf mutant Dongze 11 was obtained from Dongnong 42 induced by chemical NaN3.Through the exogenous GA3application and endogenesis GA3content measurement，Dongze 11 and Dongnong 42 were used as research materials to study into how GA3phytohormone worked in the dwarf mutant.The results indicated that the exogenous GA3could apparently promote the stem elongation of the dwarf mutant，and this effect became more significant with treating time accumulated.Among different concentrations of GA3，0.6 mmol.L-1GA3 performed especially well.The application of 2.9×10-4mmol.L-1GA3 had obvious effect on elongation of young stem in vivo，implying that the endogenesis GA3content in mutant was very low，and in this assay，the treatment of 12 hours was most effective.Endogenesis GA3contents during different growth stage was determined by ELISA(Enzyme-linked immunosorbent assay)，and results showed that the mutant had lower GA3level than the wild plant，suggesting the shortened stem in mutant should related with the lower GA3content.

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备注/Memo

基金项目：国家高技术研究发展计划“863计划”资助项目（2003AA207060-5）；黑龙江省科技攻关资助项目（PC05S107）；东北农业大学科学研究基金资助。

作者简介：张达（1977-），女，讲师，博士，主要从事植物生理生化研究。Tel：0451-55190002；E-mail：zhangda77_@163.com。

更新日期/Last Update: 2014-10-08