[1]马中雨,李俊,张永芳,等.大豆根瘤菌与大豆品种共生匹配性研究[J].大豆科学,2008,27(02):221-227.[doi:10.11861/j.issn.1000-9841.2008.02.0221]
　MA Zhong-yu,LI Jun,ZHANG Yong-fang,et al.Symbiotic Matching between Soybean Rhizobium and Soybean Cultivars[J].Soybean Science,2008,27(02):221-227.[doi:10.11861/j.issn.1000-9841.2008.02.0221]
点击复制

大豆根瘤菌与大豆品种共生匹配性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 221-227 栏目: 出版日期: 2008-04-30
Title:
Symbiotic Matching between Soybean Rhizobium and Soybean Cultivars
文章编号:
1000-9841(2008)02-0221-07
作者:
马中雨; 李俊; 张永芳; 樊蕙; 李力
中国农业科学院农业资源与农业区划研究所，北京 100081
Author(s):
MA Zhong-yu; LI Jun; ZHANG Yong-fang; FAN Hui; LI Li
Institute of Agricultural Resources and Agricultural Regional Planning，Chinese Academy of Agricultural Sciences，Beijing 10081，China
关键词:
根瘤菌; 大豆; 结瘤匹配性; 固氮酶活性
Keywords:
Rhizobium; Glycine max and soja; Matching of nodulation; Nitrogen fixation activity
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0221
文献标志码:
A
摘要:
大豆与大豆根瘤菌的共生体系是共生固氮的代表，开展根瘤菌与大豆品种的匹配性组合研究，为发挥这一共生体系的固氮效率和指导大豆育种材料的选择等方面均具有重要的实践价值。选取不同种类与来源的大豆根瘤菌代表菌株18株，与11个我国主要的大豆核心种质品种进行共生匹配性试验，测定了其中5个大豆品种与14个根瘤菌所结瘤的固氮酶活性并进行统计分析。结果表明：不同的大豆根瘤菌菌株间存在明显的结瘤固氮差异，快生大豆根瘤菌表现出比慢生根瘤菌更严格的大豆品种匹配性；其中USDA110、USDA110-A、113-2、WHG12、HH103、B16比其他供试菌株表现出广谱的结瘤特性，且USDA110、USDA110-A、113-2、WHG12、DE333、2048菌株形成的根瘤具有较高的固氮酶活性。在供试大豆品种中，商951099、郑92116和北京黑豆表现出较高的结瘤、固氮特性；商951099和郑92116这对材料与所供试18株大豆根瘤菌均结瘤且差异性不明显，而绥农14和绥农20、合丰25和固新野生大豆这两对材料表现出明显结瘤差异性。因此，实践中应根据大豆品种和大豆根瘤菌菌株的匹配性关系，选用共生效果好的菌株接种，或选用与之匹配的大豆种质材料育种，以达到提高共生固氮功效的目的。
Abstract:
The symbiotic system between soybean and soybean rhizobium is a typical representative of nitrogen fixation symbiosis.And the test of matching combination between soybean and soybean rhizobium will improve the effectiveness of symbiotic nitrogen fixation and provide basis for soybean breeding，which is very significative in practice.This paper selected 18 representatives of soybean rhizobia and 11 soybean cultivars representing soybean core germplasm varieties in China to test the symbiotic matching between soybean rhizobium and their host.The nitrogen-fixation activities of 5 representative soybean cultivars’ root nodules with 14 rhizobium strains were determined and the data were analyzed by SPSS.The results showed that the symbiotic abilities were significantly different among the 18 the rhizobia，and S.fredii exhibited more critical than B.japonicum in nodulation.The strains of USDA110、USDA110-A、113-2、WHG12、HH103、B16 were superior broad-hosted in symbiotic nodulation，and the nodules of USDA110，USDA110-A，113-2，WHG12，DE333 and 2048 had higher nitrogen-fixation activities than others.Among the 11 soybean cultivars，Shang 951099，Zheng 92116，and Beijingheidou showed higher abilities in nodulation and nitrogen-fixation activities.And the results also showed that the pair of hybrid parents Shang 951099 and Zheng 92116 had no difference with nodulation ratio，however the pairs of Suinong 14 and Suinong 20，Hefeng 25 and Guxin wild soybean，showed great differences in nodulation ability.In order to improve the effectiveness of symbiotic nitrogen fixation，we should select the corresponding strains to inoculate and choose the matching germplasm material to breed in practice.

参考文献/References:

[1]江木兰，张学江，徐巧珍，等.大豆—根瘤菌的固氮作用［J］.中国油料作物学报，2003，25(1):50-54.(Jiang M L，Zhang X J，Xu Q Z，et al.Nodulation and nitrogen-fixation in soybean-rhizobium.2003.Chinese Journal of Oil Crop Sciences，2003，25(1):50-54.)

[2]Kiers E T，Hutton M G，Denison R F.Human selection and the relaxation of legume defences against ineffective rhizobia［J］.Proceedings of The Royal Society/Biological Sciences，2007,274(1629):3119-3126.
[3]Provorov N A，Tikhonovich I A.Genetic resources for improving initrogen fixation in legume-rhizobia symbiosis［J］.Genetic Resources and Crop Evolution,2003,50：89-99.
[4]史晓霞，师尚礼，杨晶，等.2006，豆科植物根瘤菌分类研究进展［J］.草原与草坪，2006,(1):12-17.(Shi X X，Shi S L，Yang J，et al.Research advancement intaxonomy of Rhizobium leguminosarum［J］.Grassland and Turf，2006,（1）：12-17.
[5]Tan Z Y，Wang E T，Peng G X，et al.Characterization of bacteria isolated from wild legumes in the North-Western regions of China［J］.International Journal of Systematic Bacteriology，1999,49：1457-1469.
[6]Yan A M，Wang E T，Kan F L，et al.Sinorhizobium melilotii associated with Medicago sativa and Melilotus spp［J］.International Journal of Systematic Bacteriology，2000,50:1887-1891.
[7]邱丽娟，常汝镇，孙建英，等.中国大豆品种资源保存与更新状况分析［J］.中国农业科技导报，2000.3(2):34-39.(Qiu L J，Chang R Z，Sun J Y，et al.Perspects of evaluation and utiliztion of soybean germplamin China［J］.Review of China Agricultural Science and Technology，2000,3(2):34-39.)
[8]黄 芳，许修宏.不同类型土壤中大豆与根瘤菌的匹配性研究［J］.东北农业大学学报，2007,38(2)：211-214.(Huang F，Xu X H.Study on the matching of soybean and rhizobium in different types soil［J］.Journal of Northeast Agricultural University，2007,38(2)211-214.)
[9]Hardy R W F，Holsten R D Jackson E K,et al.The acetylene-ethylene assay for nitrogen fixation：laboratory and field evaluations［J］.Plant Physioloy，1968,43(8):1185-1207.[10]王福生，李臬棣，陈华葵.土壤中大豆根瘤菌之间竞争结瘤的研究Ⅲ接种菌量对大豆生长的影响.土壤学报，1989,26(4):388-392.(WANG F S，Li F D，and Chen H K.Studies on competition for nodulation between soybean rhizobia in soil Ⅲ effect of inoculation rate on soybean growth［J］.Acta Pedologica Sinica，1989,26(4):388-392.)
[11]李新民，谷思玉，窦新田，等.不同土壤大豆接种根瘤菌剂反应的研究［J］.黑龙江省农业科学，1998,(4):1-5.(Li X M，Gu S Y，Dou X T，te al.Study on plant response to inoculation with Rhizobium japonicum Strain in different soil types［J］.Heilongjiang Agricultural Science，1998,(4)：1-5.)
[12]缪礼鸿，周俊初，郑惠芬.费氏中华根瘤菌内源质粒和大豆品种对菌株竞争结瘤能力及固氮效率的影响［J］.中国农业科学，2002,35(7):802-808.(Liao L H，Zhou J C，Zheng H F.The influence of indigenous plasmids and soybean cultivar on the competitiveness for nodules of the strains and nitrogen-fixing effectiveness of Sinorhizobium fredii［J］.Scientia Agricultura Sinica，2002,35(7):802-808.)
[13]张学江，周平贞，吴生堂，等.广谱、高效的大豆根瘤菌113-2［J］.中国油料作物学报，1986,(1):64-70.(Zhang X J，Zhou P Z，Wu S T，et al.A strain of soybean rhizobium 113-2 with efficient and broad-spectrum nodulous range［J］.Chinese Journal of Oil Crop Sciences，1986,(1):64-70.)
[14]周蓉，王贤智，沙爱华，等.大豆数量性状定位的研究进展［J］.中国农业技术科学，2005,21(10):30-35.(Zhou R，Wang X Z，Sha A H，et al.Advances in QTL of soybean mapping［J］.Chinese Agricultural Science Bulletin，2005,21(10):30-35.)
[15]郑永战，盖钧镒，卢为国，等.大豆脂肪及脂肪酸组分含量的QTL定位［J］.作物学报，2006,32(12):1823-1830.(Zheng Y Z，Gai J Y，Lu W G，et al.QTL Mapping for fat and fatty acid composition contents in soybean［J］.Acta Agronomica Sinica，2006,32(12):1823-1830.)
[16]郭东全，王延伟，智海剑，等.大豆对SMV SC213株系群的抗性遗传及基因定位的研究［J］.大豆科学，2007,26(1):21-24.(Guo D Q，Wan Y W，Zhi H L，et al.Inheritance and gene mapping of resistance to SMV strain group SC-213 in soybean［J］.Soybean Sciences，2007,26(1):21-24.)

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]王宏光,孙殿君,马忠强,等.大豆根瘤菌HD001的分离鉴定及结瘤能力检测[J].大豆科学,2014,33(03):379.[doi:10.11861/j.issn.1000-9841.2014.03.0379]
　WANG Hong-guang,SUN Dian-jun,MA Zhong-qiang,et al.Isolation and Identification of Rhizobium HD001 and Its Nodulation Capacity Test in Soybean Germplasm[J].Soybean Science,2014,33(02):379.[doi:10.11861/j.issn.1000-9841.2014.03.0379]
[12]孟庆英,张春峰,于忠和,等.根瘤菌对大豆根际土壤微生物及大豆农艺性状的影响[J].大豆科学,2012,31(03):498.[doi:10.3969/j.issn.1000-9841.2012.03.035]
　MENG Qing-ying,ZHANG Chun-feng,YU Zhong-he,et al.Effects of Rhizobia on Rhizosphere Soil Microoganisms and Agronomic Characters of Soybean[J].Soybean Science,2012,31(02):498.[doi:10.3969/j.issn.1000-9841.2012.03.035]
[13]吴萍,何庆元,李正鹏,等.安徽省大豆根瘤菌表型多样性研究[J].大豆科学,2011,30(02):219.[doi:10.11861/j.issn.1000-9841.2011.02.0219]
　WU Ping,HE Qing-yuan,LI Zheng-peng,et al.Phenotypic Diversity of Soybean Rhizobia in Anhui Province[J].Soybean Science,2011,30(02):219.[doi:10.11861/j.issn.1000-9841.2011.02.0219]
[14]杨升辉,王素阁,于会勇,等.接种根瘤菌对夏大豆籽粒灌浆特性及品质的影响[J].大豆科学,2014,33(04):534.[doi:10.11861/j.issn.1000-9841.2014.04.0534]
　YANG Sheng-hui,WANG Su-ge,YU Hui-yong,et al.Effects of Rhizobial Inoculation on the Grain Filling Characteristics and Quality of Summer Soybean[J].Soybean Science,2014,33(02):534.[doi:10.11861/j.issn.1000-9841.2014.04.0534]
[15]李馨园,王守义,王淑荣,等.根瘤菌配施胶质类芽孢杆菌对大豆叶绿素荧光特性、产量及品质的影响[J].大豆科学,2014,33(04):541.[doi:10.11861/j.issn.1000-9841.2014.04.0541]
　LI Xin-yuan,WANG Shou-yi,WANG Shu-rong,et al.Effect of Rhizobium Combined with Pamibacillus mucilaginosus on Soybean Chlorophyll Fluorescence Characteristics,Yield and Quality[J].Soybean Science,2014,33(02):541.[doi:10.11861/j.issn.1000-9841.2014.04.0541]
[16]李涛,关大伟,李俊,等.黄淮海地区优良大豆根瘤菌株的筛选与接种方式研究[J].大豆科学,2010,29(04):645.[doi:10.11861/j.issn.1000-9841.2010.04.0645]
　LI Tao,GUAN Da-wei,LI Jun,et al.Screening of Superior Soybean Rhizobial Strains and Approach to Inoculation Methods for Region of HuangHuaiHai[J].Soybean Science,2010,29(02):645.[doi:10.11861/j.issn.1000-9841.2010.04.0645]
[17]张红侠,冯瑞华,关大伟,等.黄土高原地区优良大豆根瘤菌的筛选与接种方式研究[J].大豆科学,2010,29(06):996.[doi:10.11861/j.issn.1000-9841.2010.06.0996]
　ZHANG Hong-xia,FENG Rui-hua,GUAN Da-wei,et al.Screening of Superior Soybean Rhizobial Strains and Analyzing of Different Inoculation Methods in Loess Plateau Region of China[J].Soybean Science,2010,29(02):996.[doi:10.11861/j.issn.1000-9841.2010.06.0996]
[18]金晓梅,Синеговская В Т,赵念力.根瘤菌、微肥和作物生长调节剂对大豆氮磷钾积累和产量的影响[J].大豆科学,2009,28(04):751.[doi:10.11861/j.issn.1000-9841.2009.04.0751]
　JIN Xiao-mei,S inegovskaya.Influence of Rhizobium，Trace Fertilizer and Crop Growth Regulators on Nitrogen，Phosphorous，Potassium Accumulation and Yield of Soybean[J].Soybean Science,2009,28(02):751.[doi:10.11861/j.issn.1000-9841.2009.04.0751]
[19]赵宇枢,段玉玺,王媛媛,等.辽宁省大豆根瘤菌资源抗逆性及生防潜力研究[J].大豆科学,2009,28(01):113.[doi:10.11861/j.issn.1000-9841.2009.01.0113]
　ZHAO Yu-shu,DUAN Yu-xi,WANG Yuan-yuan,et al.Stress Resistance and Biocontrol Potential of Soybean Rhizobia Resourses Isolated From Liaoning Province[J].Soybean Science,2009,28(02):113.[doi:10.11861/j.issn.1000-9841.2009.01.0113]
[20]苗淑杰,乔云发,韩晓增.磷和根瘤菌交互作用对大豆结瘤和生长的影响[J].大豆科学,2009,28(02):271.[doi:10.11861/j.issn.1000-9841.2009.02.0271]
　MIAO Shu-jie,QIAO Yun-fa,HAN Xiao-zeng.Effect of Phosphorus and Rhizobium on Growth and Nodule Development in Soybean[J].Soybean Science,2009,28(02):271.[doi:10.11861/j.issn.1000-9841.2009.02.0271]

备注/Memo

基金项目：国家科技支撑项目资助（2006BAD25B04）。

作者简介：马中雨（1982-）,男，硕士研究生，研究方向为生物固氮与基因工程。

更新日期/Last Update: 2014-10-10