[1]王振东,孙仓,王惠.不同方法从大豆不同组织中提取基因组DNA效果的比较[J].大豆科学,2008,27(01):42-46.[doi:10.11861/j.issn.1000-9841.2008.01.0042]
　WANG Zhen-dong,SUN Cang,and WANG Hui.Comparison of the Genomic DNA Extraction Methods From Soybean Different Tissues[J].Soybean Science,2008,27(01):42-46.[doi:10.11861/j.issn.1000-9841.2008.01.0042]
点击复制

不同方法从大豆不同组织中提取基因组DNA效果的比较

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年01期 页码: 42-46 栏目: 出版日期: 2008-02-25
Title:
Comparison of the Genomic DNA Extraction Methods From Soybean Different Tissues
作者:
王振东; 孙仓; 王惠
沈阳农业大学生物科学技术学院，辽宁沈阳 110161
Author(s):
WANG Zhen-dong; SUN Cang; and WANG Hui
Science and Technology Department of Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
大豆基因组DNA; 提取; SDS法; CTAB法
Keywords:
Soybean genomic DNA; Extraction; SDS method; CTAB method
DOI:
10.11861/j.issn.1000-9841.2008.01.0042
文献标志码:
A
摘要:
从大豆组织中获得高质量和足够产量的基因组DNA，是进行大豆分子生物学研究的基础。为进行大豆基因组的PCR，RAPD，SSR等分子生物学研究，分别以大豆种子和其叶片为实验材料，采用改良的SDS法和CTAB法对大豆基因组DNA进行了提取。对DNA的提取效果，采用紫外分光光度检测、琼脂糖凝胶电泳分析及DNA的限制性内切酶图谱分析进行了综合比较。结果表明，在以叶片为材料时，SDS法和CTAB法的大豆基因组DNA的提取效果差别不大，SDS法稍好于CTAB法。在以大豆种子为材料时，SDS法的提取效果明显优于CTAB法，SDS法可从大豆种子中提取到能够充分满足各种分子操作的高质量和数量的大豆基因组DNA。
Abstract:
It is important to extract high quality and sufficient yield of genomic DNA for soybean molecular biological research.In order to study the soybean genome by PCR，RAPD，SSR etc.，improved SDS and CTAB method were used to extract the soybean genomic DNA from the soybean seeds and leaves.The DNA extraction effect of two different methods was compared by ultraviolet spectrophotometry，agarose electroforesis and the restriction enzyme digestion.The results showed that the extraction effect of the SDS method and the CTAB method to soybean genomic DNA was not obviously different for soybean leaves.However，the extraction effect of SDS method is much better than CTAB method for soybean seeds.The conclusion is that the SDS method is able to extract high quality and quantity soybean genomic DNA，which is suitable for various molecular operations.

参考文献/References:

[1]Chen Q S，Liu C Y，Lü D，and He J X.2004.The basic principle of DNA extraction from soybean.Journal of Northeast Agricultural University，35(2)：129-134(陈庆山，刘春燕，吕东，何建勋.2004.大豆DNA提取基本原理的探讨.东北农业大学学报，35(2)：129-134)

[2]Doyle J J，and Doyle J L.1990.Isolation of plant DNA from fresh tissue.Focus，12：13-15
[3]Guan R X，Chang R Z，and Qiu L J.2003.Rapid isolation of soybean DNA for SSR analysis.Soybean Science，22(1):73-74(关荣霞，常汝镇，邱丽娟.2003.用于SSR分析的大豆DNA的快速提取.大豆科学，22(1):73-74)
[4]Lai X H， and Wang P Z.2003.Comparison of total DNA extraction approaches and its utilization in RAPD-PCR of sunflower.Journal of Xinjiang Agricultural University，26(1):24-27(赖相红，王沛政.2003.向日葵总DNA不同提取方法比较及在RAPD中的应用研究.新疆农业大学学报，26(1)：24-27)
[5]Li W P， Zhao W M，and Lei P.2002.Rapid method for extracting DNA from plant tissues.Journal of Northwest Sci-Tech University of Agriculture and Forestry，30(6):125-128(李维平，赵文明，雷平.2002.一种少量提取植物组织DNA的快速方法.西北农林科技大学学报，30(6):125-128)
[6]Liu J， Xiong Y W，Liu G S，Qi D M，Li F F，and Wang E H.2001.DNA extraction from different part of oil sunflower mature seeds for PCR analysis.Acta Botanica Boreali-Occidentalia Sinica，21(4):615-619(刘杰，熊艳文，刘公社，齐冬梅，李芳芳，汪恩华.2001.向日葵种子不同部位微量提取DNA用于PCR的研究.西北植物学报，21(4):615-619)
[7]Peng S T， Yan Q C，Wang X D，and Zhang C R.2002.DNA extraction from single seeds and optimizing RAPD procedure in rice.Journal of Shanghai Jiaotong University ，20(1):34-41(彭锁堂，颜启传，王学德，张春荣.2002.水稻单粒种子DNA提取及其RAPD程序优化研究.上海交通大学学报，20(1):34-41)
[8] Tao G， Liu Z Y，Zhu Y，and Song J X.2004.Modification for the method of genomic DNA extraction and purification of rice and maize.Guizhou Agricultural Sciences ，32(6):21-22 (陶刚，刘作易，朱英，宋吉轩.2004.水稻玉米基因组DNA提取方法的改进.贵州农业科学，32(6):21-22)
[9]Tian J， and Luo K.2004.Fast and reliable extraction processes of total DNAs from rice.Chinese Journal of Applied Environmental Biology，10(2):143-145(田洁，罗科.2004.水稻总DNA的快速制备.应用与环境生物学报，10(2)：143-145)
[10]Wang J X， Sun Y，and Gao W J.2000.A simple of practical method for extracting plant total DNA.Journal of Shanxi University，23(3):271-272 (王景雪，孙毅，高武军.2000.一种简便实用的植物总DNA提取方法.山西大学学报，23(3):271-272)
[11]Wang Q H， Huang J，and Zhang H S.2004.A rapid method for the isolation of small amount DNA from plant leave.Letters in Biotechnology，15(5):479-480(王齐红，黄骥，张红生.2004.一种快速微量提取植物叶片DNA的方法.生物技术通讯，15(5)：479-480)
[12]Wang X D， Lv H Y，and Zhang J.2004.Comparative study on methods of extracting DNA from soybean leaf for PCR.Molecular Plant Breeding，2(6):891-894(王晓丹，吕慧颖，张敬.2004.以PCR为目的的大豆叶片DNA提取方法的比较研究.分子植物育种，2(6):891-894)
[13]Yang S H，Zhang L J，and Duan H J.2003.Rapid extraction of soybean DNA for PCR from dry seed.Soybean Science，22(2):151-153(杨少辉，张丽娟，段会军.2003.大豆种子DNA的提取方法.大豆科学，22(2):151-153)
[14]Yang W S， Wang X Y，Gou L，and Pan G T.1996.The modification of DNA purification for soybean and other plants and the research for the interfering mechanism.Journal of Sichuan Agricultural University)，14(2):153-156(杨婉身，王西瑶，苟琳，潘光堂.1996.大豆等植物材料DNA提取方法的改进及其干扰机制的探讨.四川农业大学学报，14(2):153-156)
[15]Zeng Q C，Zheng S Y，and Shen L.2004.Modification of DNA extraction protocol for Jisi Ziziphus jujuba.Letters in Biotechnology 15(2):152-153(曾强成，郑世英，沈亮.2004.金丝小枣基因组DNA的优化提取方法.生物技术通讯，15(2):152-153)
[16]Zhang Y M， Sun C Y，and Liang C Y.2000.One step isolation of plant DNA for large-scale RAPD analysis.Hereditas，22(2):106(张永明，孙彩云，梁承邺.2000.一步法提取植物DNA用于大规模RAPD分析.遗传，22(2)：106)
[17]Zhou D Y， Zhou Y F，and Pan D R.2005.DNA extraction from different soybean tissues.Journal of Fujian Agriculture and Forestry University，34(4):417-419(周德银，周以飞，潘大仁.2005.大豆不同组织材料的DNA提取.福建农林科技大学学报，34(4):417-419)
[18]Zhou S J.1999.Rapid isolation of soybean DNA for PCR with large numbers of small samples.Soybean Science，18(4);318-321(周思君.1999.小样品大批量模板DNA快速分离法.大豆科学，18(4):318-321)

相似文献/References:

[1]谷春梅,韩玲玲,曲洪生,等.大豆胰蛋白酶抑制因子的分离纯化[J].大豆科学,2013,32(01):89.[doi:10.3969/j.issn.1000-9841.2013.01.021]
　GU Chun-mei,HAN Ling-ling,QU Hong-sheng,et al.Extraction and Purification of Soybean Trypsin Inhibitor[J].Soybean Science,2013,32(01):89.[doi:10.3969/j.issn.1000-9841.2013.01.021]
[2]梁晓芳,王步军.大豆中异黄酮和皂苷的提取、制备研究进展[J].大豆科学,2014,33(01):128.[doi:10.11861/j.issn.1000-9841.2014.01.0128]
　LIANG Xiao-fang,WANG Bu-jun.Research Progress of Extraction and Preparation Methods of Soybean Isoflavones and Soyasaponins[J].Soybean Science,2014,33(01):128.[doi:10.11861/j.issn.1000-9841.2014.01.0128]
[3]谷春梅,韩玲玲,曲洪生,等.大豆胰蛋白酶抑制因子的研究进展[J].大豆科学,2012,31(01):149.[doi:10.3969/j.issn.1000-9841.2012.01.034]
　GU Chun-mei,HAN Ling-ling,QU Hong-sheng,et al.Recent Advances in Soybean Trypsin Inhibitor[J].Soybean Science,2012,31(01):149.[doi:10.3969/j.issn.1000-9841.2012.01.034]
[4]郭 建,王梦凡,齐 崴,等.响应面优化超声辅助提取大豆异黄酮联产分离蛋白和低聚糖[J].大豆科学,2012,31(04):655.[doi:10.3969/j.issn.1000-9841.2012.04.029]
　GUO Jian,WANG Meng-fan,QI Wei,et al.Optimization of Ultrasonic-assisted Extraction of Soybean Isoflavones by Response Surface Methodology and Simultaneous Preparation of Protein Isolates and Oligosaccharides[J].Soybean Science,2012,31(01):655.[doi:10.3969/j.issn.1000-9841.2012.04.029]
[5]于海莲.微波辅助乙醇提取大豆异黄酮的研究[J].大豆科学,2011,30(01):144.[doi:10.11861/j.issn.1000-9841.2011.01.0144]
　YU Hai-lian.Extraction of Isoflavonoids from Soybean by Ethanol Assisted Microwave Irradiation[J].Soybean Science,2011,30(01):144.[doi:10.11861/j.issn.1000-9841.2011.01.0144]
[6]张花利,冯进,董晓娜,等.黑豆皮中黄酮提取及粗提物抑菌效果研究[J].大豆科学,2011,30(03):497.[doi:10.11861/j.issn.1000-9841.2011.03.0497]
　ZHANG Hua-li,FENG Jin,DONG Xiao-na,et al.Extraction of Flavonoids from the Peel of Black Bean and Bacteriostatic Effect of Crude Extractings[J].Soybean Science,2011,30(01):497.[doi:10.11861/j.issn.1000-9841.2011.03.0497]
[7]熊杰,杨玥熹,华欲飞.豆渣水溶性大豆多糖提取工艺研究[J].大豆科学,2009,28(06):1119.[doi:10.11861/j.issn.1000-9841.2009.06.1119]
　XIONG Jie,YANG Yue-xi,HUA Yu-fei.Extraction Technology of Soluble Soybean Polysaccharides from Bean Dregs[J].Soybean Science,2009,28(01):1119.[doi:10.11861/j.issn.1000-9841.2009.06.1119]
[8]徐启红,任平国.豆渣中水溶性大豆多糖的提取工艺研究[J].大豆科学,2008,27(05):902.[doi:10.11861/j.issn.1000-9841.2008.05.0902]
　XU Qi-hong,REN Ping-guo.Extraction Technique of Soluble Soybean Polysaccharide from Soybean Dregs[J].Soybean Science,2008,27(01):902.[doi:10.11861/j.issn.1000-9841.2008.05.0902]
[9]周泉城,申德超,区颖刚.微波辅助提取大豆甙元工艺研究[J].大豆科学,2008,27(01):124.[doi:10.11861/j.issn.1000-9841.2008.01.0124]
　ZHOU Quan-cheng,SHEN De-chao,and QU Ying-gang.Microwave-assisted Extraction of Daidzein from Defatted Soybean Meal after Extrusion[J].Soybean Science,2008,27(01):124.[doi:10.11861/j.issn.1000-9841.2008.01.0124]
[10]谭永辉,王文生,秦玉昌,等.豆渣中水溶性大豆多糖的提取与应用[J].大豆科学,2008,27(01):150.[doi:10.11861/j.issn.1000-9841.2008.01.0150]
　TAN Yong-hui,WANG Wen-sheng,QIN Yu-chang,et al.Extraction and Application of Soluble Soybean Polysaccharides from Bean Curd Waste[J].Soybean Science,2008,27(01):150.[doi:10.11861/j.issn.1000-9841.2008.01.0150]

备注/Memo

基金项目：沈阳农业大学青年教师科研基金（2005006）

作者简介：王振东（1956-），男，教授，博士，博士生导师，从事生物技术教学与研究。Tel: 024-88487163; E-mail: zhendongwang1212@yahoo.com.cn
通讯作者(Corresponding author)：王惠，博士。Tel:024-88487163; E-mail: wanghuisynd@yahoo.com.cn

更新日期/Last Update: 2014-10-09