[1]刘水丽,杨其长.无糖培养条件下三种大豆组培苗生长差异研究[J].大豆科学,2007,26(02):161-166.[doi:10.3969/j.issn.1000-9841.2007.02.009]
　LIU Shui-li,YANG Qi-chang.GROWTH DIFFERENCES IN THREE SOYBEAN VARIETIES PLANTLETS UNDER PHOTOAUTOTROPHIC CONDITION[J].Soybean Science,2007,26(02):161-166.[doi:10.3969/j.issn.1000-9841.2007.02.009]
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无糖培养条件下三种大豆组培苗生长差异研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年02期 页码: 161-166 栏目: 出版日期: 2007-04-25
Title:
GROWTH DIFFERENCES IN THREE SOYBEAN VARIETIES PLANTLETS UNDER PHOTOAUTOTROPHIC CONDITION
文章编号:
1000-9841(2007)02-0163-04
作者:
刘水丽; 杨其长
中国农业科学院农业环境与可持续发展研究所，北京 100081
Author(s):
LIU Shui-li; YANG Qi-chang
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricul-tural Sciences, Beijing 100081
关键词:
无糖培养; 大豆; 生根
Keywords:
Sugar free tissue culture; Soybean; Rooting
分类号:
S565.103.53
DOI:
10.3969/j.issn.1000-9841.2007.02.009
文献标志码:
A
摘要:
在可控环境下，以有糖培养为对照，对中黄13、中黄25和鑫豆1号三个大豆品种进行了无糖培养试验，探讨了三个品种的组培苗的生长差异和根系活力差异。结果表明，生根培养21 d后，有糖培养处理的大豆组培苗的叶片部分出现黄化现象，无糖培养条件下三种大豆组培苗生长健康，叶色浓绿，生根率均为100％（高于有糖培养），中黄25的根鲜重与根长指标达到最大值，分别为1.02 g和481.6 cm，鑫豆1号的根系活力最高，达到78.62 UTTC-1FWh-1，中黄13与鑫豆1号的根系活力与其有糖培养处理相比差异显著。因此，无糖培养提高了大豆组培苗对环境的适应能力，促进了组培苗的生长发育。
Abstract:
The aim of the study was to take the photoautotrophic culture experiment in three soybean varieties (Glycine max L.) Zhonghuang 13, Zhonghuang 25 and Xindou 1 under controlled environment, discuss the growth and root activities differences of them. It was shown that some leaves of the soybean plantlets under conventional culture appeared etiolation, while the tops of the three varieties plantlets were healthy and their leaves were dark green under photoautotrophic environment whose rooting percentages all reached to 100%, the root fresh weight and the root length of Zhonghuang25 achieved the maximum values, which were 1.02 g and 481.6 cm respectively，and the root activity of Xindou 1 was the best, which was 78.62 UTTCg-1FW.h-1. There were significant differences between photoautotrophic and conventional culture in root activity of Zhonghuang 13 and Xindou 1. We can draw a conclusion that photoautotrophic culture enhances the adaptability of soybean plantlets to the natural environment, and improves the growth of them.

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备注/Memo

基金项目：科技部科学仪器升级改造专项（JG－2003－6）；中国农业科学院基金项目资助

作者简介：刘水丽（1981－），女，硕士，环境工程专业。

更新日期/Last Update: 2014-10-21