[1]李小平 　甘　睿 　马媛媛 　李鹏丽 　张丽文 王 　勇 　张　韧 　王宁宁 **.一个新的大豆类受体蛋白激酶基因 rlpk2 的克隆及其结构特征[J].大豆科学,2005,24(04):249-255.[doi:10.11861/j.issn.1000-9841.2005.04.0249]
　Li Xiaoping Gan Rui Ma Yuanyuan Li Pengli Zhang LiwengWang YongZhang RenWang Ningning**.CLONE AND CHARACTERISTICS OF A NOVELSOYBEAN RECEPTOR- LIKE PROTEINKINASE rlpk 2 GENE*[J].Soybean Science,2005,24(04):249-255.[doi:10.11861/j.issn.1000-9841.2005.04.0249]
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一个新的大豆类受体蛋白激酶基因 rlpk2 的克隆及其结构特征

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年04期 页码: 249-255 栏目: 出版日期: 2005-11-25
Title:
CLONE AND CHARACTERISTICS OF A NOVELSOYBEAN RECEPTOR- LIKE PROTEINKINASE rlpk 2 GENE*
作者:
李小平 1 　甘　睿 1 　马媛媛 1 　李鹏丽 1 　张丽文 1王 　勇 1 　张　韧 2 　王宁宁 1 **
(1. 南开大学植物学及生态学系, 天津 300071 ;2. Department of Biological Sciences , University of Wollongong , NSW2522 , Australia )
Author(s):
Li Xiaoping 1 　Gan Rui 1 　Ma Yuanyuan 1 　Li Pengli 1 　Zhang Liweng1Wang Yong1　Zhang Ren2　Wang Ningning1**
(1.Nan Kai University Plant Biology and Ecology Department Tianjin 300071 ;2.Department of Biological Sciences, University of Wollongong , NSW2522, Australia)
关键词:
大豆; 末端转移酶 ; 类受体蛋白激酶 ; 快速扩增 cDNA 末端
Keywords:
Soybean ; Terminal deoxynucleotidyl Transferase (TdT); Receptor - like protein ki-nase ; Rapid Amplification of a cDNA Ends (RACE
DOI:
10.11861/j.issn.1000-9841.2005.04.0249
文献标志码:
A
摘要:
　植物类受体蛋白激酶在细胞生长及对环境胁迫反应方面起着非常重要的作用 。本文报告用RACE 方法(快速扩增 cDNA 末端)扩增一个新的大豆类受体蛋白激酶基因 rlpk2 的全长 cDNA 。根据 RACE 的结果,我们克隆了 rlpk2的cDNA ,其序列信息已经提交到GenBank (Accession No.AY687391)。经初步分析, rlpk 2 编码产物为 LRR型类受体蛋白激酶。
Abstract:
　Receptor - like protein kinases (RLKs) play major roles in plant cellular processes andstress responses. Here we reported rapid amplification of cDNA ends( RACE)of a novel soybeanreceptor - like kinase gene rlpk 2. Full length rlpk 2 cDNA was cloned according to the results ofRACE. Details of PCR condition were discussed. The resultant cDNA sequence has been submit-ted into the GenBank database and assigned the accession No. AY687391.

参考文献/References:

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备注/Memo

基金项目:国家自然科学基金资助(No. 30270140)

作者简介:李小平(1972 -), 男, 讲师, 研究方向植物分子生物学。
**通迅作者:E - mail :Wangnn@nankai. edu. cn

更新日期/Last Update: 2017-01-08