[1]高学文杨春樊建坤丁俊杰郑天琪马淑梅.大豆与灰斑病菌互作早期防卫酶及可溶性蛋白的表达特征[J].大豆科学,2003,22(03):193-196.[doi:10.11861/j.issn.1000-9841.2003.03.0193]
　Gao Xuew enYang ChunFan JiankunDing JunjieZheng TianqiMa Shumei.THE EXPRESSION CHARACTER OF DEFENSE ENZYMES ANDSOLUBLE PROTEINS IN THE EARLY INTERACTION BETWEENSOYBEAN AND Cercospara soj ina[J].Soybean Science,2003,22(03):193-196.[doi:10.11861/j.issn.1000-9841.2003.03.0193]
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大豆与灰斑病菌互作早期防卫酶及可溶性蛋白的表达特征

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第22卷 期数: 2003年03期 页码: 193-196 栏目: 出版日期: 2003-08-25
Title:
THE EXPRESSION CHARACTER OF DEFENSE ENZYMES ANDSOLUBLE PROTEINS IN THE EARLY INTERACTION BETWEENSOYBEAN AND Cercospara soj ina
文章编号:
1000 -9841(2003)03 -0193 -04
作者:
高学文1杨春1樊建坤1丁俊杰2郑天琪2马淑梅2
1.南京农业大学农业部病虫监测与治理重点开放实验室, 南京210095 ;
2.黑龙江省农业科学院合江农业科学研究所, 佳木斯154007
Author(s):
Gao Xuew en1Yang Chun1Fan Jiankun1Ding Junjie2Zheng Tianqi2Ma Shumei2
1 .Key Laboratory of Moni toring and Management of Plant Diseases and Insects , Ministry ofAgriculture , Nanjing Agricultural University , Nanjing 210095 ;
2 .Inst itute of Hejiang Agricul tralScience Research , Hei longjiang Academy of Agricul tral Sciences , Jiamusi 154007
关键词:
大豆; 灰斑病菌; 互作; 防卫酶; 同工酶; 可溶性蛋白
Keywords:
Soybean ; Cercospora sojina ; Interaction ; Defense enzymes ; Isoenzyme ; Soluble protein
分类号:
S 432.2 + 3
DOI:
10.11861/j.issn.1000-9841.2003.03.0193
文献标志码:
A
摘要:
用大豆同一品种与不同灰斑病菌生理小种和不同品种与同一生理小种互作, 测定了大豆叶片防卫酶及可溶性蛋白的表达特征。结果表明, 大豆品种与不同亲和性的小种互作后POD 和SOD 活性都有增强, 但非亲和互作中防卫酶活性增加的速度和强度显著高于亲和互作。POD 和SOD 同工酶谱带仅发生强弱变化, 未见有数量改变, 说明POD 和SOD 活性的增加与同工酶活性的增强有关。可溶性蛋白电泳图谱显示, 在非亲和或亲和互作中大豆某些可溶性蛋白谱带有所增强,是一些病程相关蛋白。而大豆不同品种与灰斑病菌同一生理小种互作后POD 快速而显著表达也与大豆和灰斑病菌的非亲和性相关。上述结果表明防卫酶POD 和SOD 活性的快速而显著增高是大豆与灰斑病菌非亲和互作的特征。
Abstract:
Expression character of defense enzymes and soluble proteins in the interaction betw een soybean sing lev ariety and Cercospora sojina dif ferent strains or dif ferent varieties and single st rain w ere assayed .Result sshowed that POD and SOD activity in both incompatible or compatible interaction were increased , but the increaseof POD and SOD activity in incompat ible interaction was higher and faster than that in compatible interaction, w hile the number of isoenzyme bands w as not changed in both interaction , that implied the increase ofPOD and SOD activity w as related w ith increase of isoenzymes act ivity .Electrophoresis show ed that some solubleproteins w ere increased in both incompatible and compatible interaction , they are pathogenesis -related proteins(PR protein).The fast and marked expression of POD activity in the soybean varieties w hich w as inoculatedw ith sing le st rain is also related w ith incompat ibility of soybean and Cercospara soj ina .The results indicatethat fast and marked increase of defence enzymes POD and SOD activity is a character of incompatible interact ionbetween soybean and Cercospara sojina .

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备注/Memo

基金项目:霍应东青年教师基金(81027)。　

作者简介:高学文(1965 -), 男, 副教授, 博士, 从事植物与病原物互作分子机理和植物病害生物防治研究。E -mail:gaoxuew @yahoo .

更新日期/Last Update: 2016-12-31