WANG Shao-dong,LI Yuan-ming,LIU Wei,et al.A Fast Detection Method for Screening of Gly m Bd 30 K Allergen Protein Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean Seeds[J].Soybean Science,2011,30(04):638-642.[doi:10.11861/j.issn.1000-9841.2011.04.0638]
聚丙烯酰胺凝胶电泳快速检测大豆过敏原蛋白Gly m Bd 30 K缺失方法
- Title:
- A Fast Detection Method for Screening of Gly m Bd 30 K Allergen Protein Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean Seeds
- 文章编号:
- 1000-9841（2011）04-0638-05
- Keywords:
- Allergen proteins; Sodium dodecyl sulfate polyacrylamide gel electrophoresis; Western blotting; Soybean seeds
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 通过对现有SDS-PAGE电泳技术的样本前处理和制胶技术的改良，开发出了一种能够同时清晰鉴别7S球蛋白组分亚基（α’,α,β-Subunit）和Gly m Bd 30 K谱带的SDS-PAGE电泳快速检测新技术。利用该方法对PI603570A×0329F6杂交组合F2群体的116个样本进行了7S球蛋白组分亚基（α’,α,β-Subunit and Gly m Bd 30K）缺失的筛选，并通过回交转育技术，将7S球蛋白组分亚基的缺失性状成功导入到黑龙江主栽品种中去。新方法可以有效地加快筛选进程，降低筛选成本，对过敏原蛋白缺失大豆种质创新具有重要意义。
- Abstract:
- In this research,through the improvement of the sample pretreatment and confection of the gel,a fast detection method was developed for simultaneous determination of α’,α,β-Subunit and Gly m Bd 30 K lacking individual using sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)in soybean seeds.We have screened F2(PI603570A×0329F6)population(N=116)in 7S globuline lacking by this method.Moreover,we have introduced the lacking character into the major soybean varieties in Heilongjiang province through the backcross breeding.The results indicated that the method was better in velocity and less costs of the screening.It is significant for the development of soybean germplasm in the allergen proteins lacking.
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相似文献/References:
[1]王绍东,姜妍,王浩,等.新聚丙烯酰胺凝胶电泳快速检测大豆脂氧酶缺失方法[J].大豆科学,2011,30(03):484.[doi:10.11861/j.issn.1000-9841.2011.03.0484]
WANG Shao-dong,JIANG Yan,WANG Hao,et al.A Fast Detection Method for Screening of Lipoxygenase Null Individual Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis in Soybean[J].Soybean Science,2011,30(04):484.[doi:10.11861/j.issn.1000-9841.2011.03.0484]
备注/Memo
基金项目：哈尔滨市科技创新人才研究专项资金资助项目（2010RFLYN016）；东北农业大学留学回国基金资助项目（2010RCA03）；黑龙江省留学回国基金资助项目（LC2009C25）。