[1]赵桂兰　刘艳芝　李俊波　徐洪志　刘莉　尹爱平.影响农杆菌介导的大豆基因转化因素的研究[J].大豆科学,2001,20(02):84-88.[doi:10.11861/j.issn.1000-9841.2001.02.0084]
　Zhao Guilan Liu Yanzhi Li Junbo Xu Hong zhi Liu Li Yin Aiping.STUDY ON FACTORS LNFLUENCING AGROBACTERIUM-MEDIAGEDSOYBEAN TRANSFORMATION[J].Soybean Science,2001,20(02):84-88.[doi:10.11861/j.issn.1000-9841.2001.02.0084]
点击复制

影响农杆菌介导的大豆基因转化因素的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第20卷 期数: 2001年02期 页码: 84-88 栏目: 出版日期: 2001-05-25
Title:
STUDY ON FACTORS LNFLUENCING AGROBACTERIUM-MEDIAGEDSOYBEAN TRANSFORMATION
文章编号:
1000 -9841(2001)02 -0084 -05
作者:
赵桂兰　刘艳芝　李俊波　徐洪志　刘莉　尹爱平
吉林省农业生物技术重点开放实验室公主岭136100
Author(s):
Zhao Guilan 　Liu Yanzhi 　Li Junbo 　Xu Hong zhi 　Liu Li 　Yin Aiping
Biotechnology Laboratory , J il in Academy of Agricultural Science , Gongzhuling136100
关键词:
大豆; 农杆菌介导法; Barnase 基因; 基因转化
Keywords:
Soybean ; Ag robacterium -mediated ; Barnase gene ; Transformation
分类号:
S565.S336
DOI:
10.11861/j.issn.1000-9841.2001.02.0084
文献标志码:
A
摘要:
通过农杆菌介导法用含NPT -Ⅱ和Barnase 基因导入到大豆幼胚, 大豆子叶节中, 然后经过含Kan 的筛选培养基中连续筛选, 获得一批Barnase 转基因植株。经PCR 扩增结果, 证实此基因已整合到大豆中, 在当代植株中生物学特性观察。花粉粒不育率在98 %。
Abstract:
Npt -II and Barnase gene w as int roduced into soybean(Glycine max (L .)Merr .)immature cotyledonousand cotyledon nodes by gene transformation mediated by Agrobacterium tumefaciens .Kanamycin resistant(kmR)plantlets w ere obtained by 3 -4 cycles screening in selective condition .Thepresence of banase gene in the transgenic plants w ere confirmed by PCR .Result s of PCR analysis proved that T-DNA was integ rated into the genome of soybean .Alterant plants positive for barase gene , the pollen grains incomplete male sterile were 98 %.Facto rs influencing agrobacterium -mediated soy bean t ransfo rmation w ere investigated

参考文献/References:

1　Harold N .Trick , Randy D.Dinkins , Eliane R.Santarem et al., Recentadvancesin soybean t ransformat ion[ J] .Plant Tissue Cultu re andBiotechnology .1997 , Vol.3 No .1 , 9 -26

2　Mari ani C ., De Beuckeleer M , Truet tner J et al., Induction of malest erility in plant by a chimaeri c ribonuclease gene[ J] .Nature, 1990 ,347 :737 -741
3 　Mariani C , Gossele V , Beuckeleer M , et al., A chimaeric ribonucleaseinhibit or gene restores fertility t o male st erile plant s[ J] .Natu re ,1992 , 357 :384 -387
4　刘艳芝, 赵桂兰, 刘莉, 等.大豆幼胚子叶诱导胚胎发生[ J] .吉林农业科学, 1999 , 24(6):16 -18
5 　刘艳芝, 庄炳昌, 赵桂兰.大豆子叶节组培耐盐突变体筛选[ J] .吉林农业科学, 1998 , 4 :38 -3
6 　刘莉, 赵桂兰.大豆子叶节组织培养再生研究[ J] .吉林农业科学, 1999 , 24(5):16 -19
7 　Jeff erson , R,A ., 1987 , Assaying Chimeric genes in plan ts the gusfusion system[ R] .Plant Mol.Biol.Rep .5 :389 -405

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(02):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(02):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(02):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(02):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]卜云萍　李明春　胡国武　王广科　邢来君.向大豆导入深黄被孢霉■6 -脂肪酸脱氢酶基因的初步研究[J].大豆科学,2003,22(01):11.[doi:10.11861/j.issn.1000-9841.2003.01.0011]
　Bu Yunping Li Mingchun Hu Guowu Wang Guangke Xing Laijun.A PRELIMINARY STUDY ON TRANSFERRING ■6 -FATTY ACID DESATURASEGENE FROM MORTIERALLA ISABELLINA INTO SOYBEANS[J].Soybean Science,2003,22(02):11.[doi:10.11861/j.issn.1000-9841.2003.01.0011]
[12]张小明,薛永国,王萍,等.转TaNHX2基因大豆的抗旱性鉴定[J].大豆科学,2017,36(04):519.[doi:10.11861/j.issn.1000-9841.2017.04.0519]
　ZHANG Xiao-ming,XUE Yong-guo,WANG Ping,et al.Identification of Drought Resistance to TaNHX2Transgenic Soybean[J].Soybean Science,2017,36(02):519.[doi:10.11861/j.issn.1000-9841.2017.04.0519]

备注/Memo

基金项目:国家八六三计划项目。

作者简介:赵桂兰(1953 -), 女, 研究员, 主要从事大豆及豆科牧草遗传转化研究。

更新日期/Last Update: 2016-12-28