[1]袁鹰刘德璞郑培和徐文静李海龙.大豆组织培养再生植株研究[J].大豆科学,2001,20(01):9-13.[doi:10.11861/j.issn.1000-9841.2001.01.009]
　Yuan YingLiu DepuZheng PeiheXu WenjingLi Hailo ng.STUDY ON PLANT REGENERATION FROM SOYBEAN CULTURE[J].Soybean Science,2001,20(01):9-13.[doi:10.11861/j.issn.1000-9841.2001.01.009]
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大豆组织培养再生植株研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第20卷 期数: 2001年01期 页码: 9-13 栏目: 出版日期: 2001-02-25
Title:
STUDY ON PLANT REGENERATION FROM SOYBEAN CULTURE
文章编号:
1000- 9841( 2001) 01- 0009- 05
作者:
袁鹰1刘德璞1郑培和1徐文静1李海龙2
1.吉林省农业科学院生物重点开放实验室,公主岭136100;
2. 吉林省农业机械化学校,公主岭136100
Author(s):
Yuan Ying1Liu Depu1Zheng Peihe1Xu Wenjing1Li Hailo ng2
1. Jil in Province Key Open Labor On Agro- Biotechnology ;
2. J iLin Mechanizat ion in Agriculture School , Jil in Province
关键词:
大豆;  子叶节;  丛生芽;  植株再生
Keywords:
Soy bean;  Co tyledo n;  Multiple shoot;  Plant reg eneratio n
分类号:
S565. 035. 3
DOI:
10.11861/j.issn.1000-9841.2001.01.009
文献标志码:
A
摘要:
不同基因型的大豆子叶节在添加适宜BA和IBA的1 /2MS培养基上经过二周的预培养和二周芽诱导培养,可获得高频率的丛生芽,及时将丛生芽从高浓度的BA转至低浓度BA培养时芽可长出植株,再转至添加N AA的生根培养基上形成完整的植株。经过40- 60天的培养,每个外植体可得到20- 30个芽。芽增殖数和植株再生率因基因型、激素、切割位点、诱导时间等不同而有明显差异。初步确立了丛生芽发生和植株再生的适宜条件和程序,建起了以子叶节为外植体适合用于农杆菌介导和基因枪方法进行遗传转化的高频再生体系。
Abstract:
High f requency multiple shoo t- bud fo rmatio n f rom di fferent g eno type of soybea n co tyledonnode fo r the tw o- w eek pre- culture and two- week shoo t- inducing culture on a 1 /2MS- medium supplementedwi th BA and IBA w as o btained. Plant can be o btained f rom mul tiple sho ot- bud when t ra nsferredto low concentration o f BA, and af terw ards to f resh roo ting medium supplement N AA. 20- 30 budsw ere o btained f rom per ex pla nt fo r 40- 60 day s culture. It w as found that the plant reg eneratio n f requencyva ries obviously from g eno ty pes, hormo nes , dif ferent ex cision ci te a nd inducing time . Sui table conditionand procedure w ere established for the clump buds fo rmatio n and plant reg eneration, and this high f requencyreg eneratio n system ca n be applied to the soybean g enetic t ransfo rmatio n by ag o ra- bacterium a ndparticle bombardment.

参考文献/References:

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备注/Memo

作者简介: 袁鹰( 1964- ) ,女,助理研究员,从事植物组织培养、分子育种及植物转基因研究。

更新日期/Last Update: 2016-12-28