盐碱条件下微杆菌q2-5对大豆生长与生理特性的影响
- Title:
- Influence of Microbacterium q2-5 on Growth and Physiological Characteristics of Soybean under Saline-Alkali Conditions
- 摘要:
- 为探究植物根际促生菌在盐碱条件下对大豆生长的缓解作用，选取实验室前期分离得到的微杆菌（Microbacterium）q2-5，对其进行抗性分析和促生特性分析，抗性鉴定分析包括抗生素鉴定、耐盐性鉴定、耐碱性鉴定和耐盐碱性鉴定。促生特性分析包括分泌IAA能力、溶磷能力和固氮酶活性。同时分析在盐碱条件下接种q2-5对大豆生长状况和生理指标的影响。抗生素抗性鉴定结果表明，菌株q2-5对红霉素（ERY）、新霉素硫酸盐（NEO）、卡那霉素（Km）、壮观霉素（Spe）和庆大霉素（Gm）具有抗性。耐盐性鉴定结果表明，菌株q2-5可以在NaCl浓度为700 mmol·L-1的TY固体培养基上正常生长。耐碱性鉴定结果表明，菌株q2-5可以在pH10.1的TY固体培养基上正常生长。混合盐碱鉴定结果表明，菌株q2-5可以在混合盐碱浓度50 mmol·L-1的TY固体培养基上正常生长。促生特性分析结果显示，菌株q2-5的IAA产生量为0.07 mmol·L-1，溶磷量为0.33 mg·L-1，固氮酶活性为243.8 U·L-1。盐碱条件下分别在大豆品种合丰50和黑农91上接种微杆菌q2-5进行盆栽试验，结果显示，与不接菌相比，接种q2-5大豆幼苗的株高、茎粗、地上部分干鲜重和地下部分干鲜增加了23.94%、4.97%、33.74%、50.23%、33.31%和43.31%（合丰50），17.63%、4.60%、31.15%、48.79%、30.87%和42.15%（黑农91），且大豆幼苗根系及叶片的抗氧化酶活性、可溶性蛋白含量和脯氨酸含量均显著增加，O2-含量和丙二醛含量也存在显著降低情况。综上所述，微杆菌q2-5通过增强大豆的抗氧化能力和渗透调节能力，有效缓解了盐碱胁迫对植株造成的生理伤害，为利用微生物菌剂改良盐碱地大豆栽培提供了理论依据和应用潜力。
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[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(02):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
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LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(02):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
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DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(02):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
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ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(02):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
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GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(02):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
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WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(02):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
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