大豆TIFY基因家族的生物信息学分析及非生物胁迫表达研究
- Title:
- Bioinformatics Analysis of the TIFY Gene Family of Soybean (Glycine max L.) and the Expression Under Abiotic Stress
- 摘要:
- TIFY是一类植物特有的转录因子，参与植物的生长发育和逆境胁迫反应。目前，已在拟南芥和水稻等植物中报道了一些参与逆境胁迫的TIFY基因，但大豆中相关研究仍较少。为了明确大豆TIFY基因家族的蛋白质理化性质、基因结构、启动子顺式作用元件、基因进化以及表达特征，对其进行生物信息学分析，并进行转录组数据分析及干旱和盐胁迫qRT-PCR验证。结果显示：获得了38个大豆TIFY家族基因，分布于16条染色体上，分为4个亚组，有10对片段重复基因和1对串联重复基因。GmTIFY蛋白均为亲水性蛋白，平均氨基酸序列长度为280.9 aa。启动子预测结果揭示GmTIFY基因启动子序列中富含光信号与激素和胁迫响应相关的顺式作用元件，表明TIFY家族基因可能参与大豆的生长发育调节过程。组织表达模式分析结果表明，GmTIFY在多个组织中表达差异显著，大多数基因在所有组织中的表达水平相对较低，甚至不表达。通过转录组数据分析及qRT-PCR实验发现，Glyma.01g204400、Glyma.05g235500、Glyma.09g077500、Glyma.13g112000、Glyma.15g179600和Glyma.15g184900基因在干旱和盐胁迫下均上调表达，这些结果为深入研究大豆TIFY家族基因功能提供了参考。
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