基于CIELAB的大豆种皮颜色全基因组关联分析
- Title:
- Genome-Wide Association Study of Soybean Seed Coat Color Based on CIELAB
- 关键词:
- CIELAB色彩空间; 大豆; 种皮颜色; 全基因组关联分析
- 摘要:
- 为探究基于色差仪的CIBLAB量化方法在大豆种皮颜色遗传多样性分析中的应用潜力，本研究以274份大豆核心种质作为关联群体，基于CIBLAB方法，利用色差仪进行种皮颜色测定，获得6个颜色参数。利用主成分分析，明确表征大豆种皮颜色的最佳参数。在此基础上，通过遗传多样性和全基因组关联分析，挖掘与大豆种皮颜色显著关联的SNP位点。主成分分析表明，大豆种皮颜色主要包括黄、绿、黑和其他等4个类型，其中黄色占比较大，且黄蓝色度值（b*）是表征大豆种皮颜色的最佳参数。遗传多样性分析表明，黄色相关性状在品种间表现出丰富的遗传变异，变异系数为-35.53%～48.19%。对172份黄色种皮大豆全基因组关联分析，共检测到462个与b*显著相关的SNP位点，包括287个新位点和175个与已报道的I和L1位点重合的位点。综上，利用色差仪精准量化种皮颜色是一种能够客观评估大豆种皮颜色的新方法。利用该方法，检测到一些与大豆种皮颜色显著关联的新SNP位点，有助于进一步解析调控大豆种皮颜色的遗传机制。
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