大豆miR10193家族成员鉴定及其结瘤相关表达分析
- Title:
- Systematic Identification of miR10193 Family Members and Nodulation Relavant Expression in Soybean
- 摘要:
- miR10193是在大豆中和线虫侵染等相关的一个miRNA家族，其在大豆共生结瘤中的作用仍不清楚。为系统鉴定大豆miR10193家族成员并分析其在共生固氮过程中的表达模式，本研究在全基因组水平上鉴定大豆miR10193家族成员并进行生物信息学分析，采用qRT-PCR方法分析其在不同大豆组织和根瘤菌侵染早期的表达模式。结果显示：共鉴定出12个miR10193成员，分别位于第3、4、7、8、10、13、16、19和20号染色体上。miR10193家族成员成熟序列和星标序列高度保守，它们的前体序列均可生成稳定的二级结构。miR10193家族前体序列的进化分析可将miR10193家族成员分为两个亚家族，其中gma-miR10193c和gma-miR10193h存在串联复制。顺式调控元件分析发现该家族成员可能参与植物激素、胁迫、生长和发育等过程。miR10193家族成员在不同组织的表达量分析结果表明，多个miR10193家族成员在根瘤中表达最高，表明其可能参与大豆共生结瘤的调控。除gma-miR10193d/j/k/l外，其它miR10193家族成员均在根瘤中高表达，并发现miR10193家族成员均显著响应根瘤菌的侵染。靶基因预测表明BTB/POZ结构域蛋白和2OG-Fe(Ⅱ)氧化酶家族蛋白可能是miR10193的靶基因。本研究为研究大豆miR10193家族及其在豆科结瘤固氮过程中的功能和机制提供了理论基础。
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