大豆响应盐胁迫NAC转录因子的鉴定
- Title:
- Identification of NAC Transcription Factors Response to Salt Stress in Soybean
- 摘要:
- 盐胁迫是影响大豆生长发育和产量的重要环境因素之一。NAC转录因子家族是植物响应盐胁迫的重要基因家族，但在大豆中其功能尚未被完全解析。为了挖掘控制大豆耐盐的新基因，本研究首先利用全基因组分析和聚类分析，并结合盐胁迫下大豆植株的转录组数据，对大豆受盐胁迫诱导表达的NAC基因进行分析；其次，对在根中受盐胁迫诱导表达的NAC基因进行单倍型、耐盐指数和核酸多态性分析。结果显示：在大豆基因组中共鉴定出182个NAC基因，将其分成7个亚家族；18个NAC基因受盐胁迫诱导显著上调表达，其中有8个在根中表达水平较高，可能参与响应盐胁迫。我们鉴定到NAC23的一个优异单倍型Hap1，能够显著促进大豆对盐胁迫的耐性。同时，发现NAC23的Hap1单倍型在栽培大豆品种中受到了强烈的人工选择。以上研究结果为更加深入地解析NAC家族基因与大豆耐盐性之间的关系提供了新的见解，为培育高效抗逆大豆新品种提供基因资源和新的思路。
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