转录因子GmPTF1促进大豆结瘤固氮功能研究
- Title:
- Functional Analysis of Transcription Factor GmPTF1 for Facilitating Nodulation and Nitrogen-fixation in Soybean
- 摘要:
- 大豆根瘤固氮是高耗能过程，需大量磷素，缺磷影响根瘤发育与氮素固定。转录因子GmPTF1具有提高植物耐低磷功能，为探讨其在结瘤固氮中的作用，本研究克隆根瘤GmPTF1，分析其上游启动子调控元件，研究基因时空表达，分析其在不同磷素处理下对大豆结瘤固氮的影响，明确基因功能。结果显示：GmPTF1启动子序列含根瘤特异表达元件，qRT-PCR证实其在根瘤优势表达，且低磷处理下的表达量高于正常磷处理，说明该基因与根瘤有关且响应低磷胁迫；进一步利用超表达和RNAi转基因复合植株分析发现，GmPTF1超表达可显著提高低磷胁迫条件下转基因大豆复合植株的根瘤数量（32.3%）、鲜重（31.6%）、固氮酶活性（32.2%）以及磷含量（36.6%），而RNAi植株的根瘤则呈现相反趋势，其根瘤数量、鲜重、固氮酶活和磷含量分别降低27.4%、36.7%、24.3%和29.0%。GmPTF1结合基序E-box结瘤固氮基因在超表达转GmPTF1根瘤中的表达分析发现，与对照相比，上述基因表达量显著上升，暗示GmPTF1可能通过调控上述含E-box基因促进大豆结瘤固氮。因此，本研究明确了GmPTF1转录因子具有促进大豆结瘤固氮的功能，为实现结瘤固氮分子遗传改良提供了基因资源。
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