CUI Yu-xing,BAI Ying-xue,WANG Qiu-shi,et al.Identification of Soybean Leaf Spot Pathogen Epicoccum sorghinum in Beijing[J].Soybean Science,2022,41(03):308-313.[doi:10.11861/j.issn.1000-9841.2022.03.0308]
北京地区大豆叶斑病病原菌高粱附球菌的鉴定
- Title:
- Identification of Soybean Leaf Spot Pathogen Epicoccum sorghinum in Beijing
- 文献标志码:
- A
- 摘要:
- 北京地区大豆叶斑病发生严重，发病范围较广，对大豆生产造成严重影响。为分析北京地区大豆叶斑病病害发生原因，进而为病害高效防控提供理论依据，本研究分析其田间病害症状，收集发病植株，采用组织分离法对大豆病叶进行分离培养，并采用琼胶平板表面单孢分离法对分离到的病原菌进行纯化。参照科赫法则进行致病性检测，通过形态学和分子生物学鉴定确定该病原菌的种类。结果表明：病原菌菌落初期为白色或灰白色，后期整个菌落呈现红褐色，菌落中心出现橘红色、粉红色或白色的凸起，边缘为灰白色，呈现不规则状。分生孢子为球形、近球形或卵圆形，中间有隔，多为串生，大小约为20 μm × 5 μm。结合分子生物学鉴定结果，该病原菌确定为高粱附球菌。本研究初次在大豆中分离得到高粱附球菌，并且经过回接验证发现该病原菌致病性极强。
- Abstract:
- Soybean leaf spot disease occurs with a wide range in Beijing， having a serious impact on soybean production. In order to analyze the causes of soybean leaf spot disease in Beijing， thus providing a theoretical basis for the efficient prevention and control of the disease， in this study， we analyzed the symptoms of the disease in the field， collected the diseased plants and isolated the pathogenic bacteria by tissue separation method， and purified the pathogenic bacteria by single spore separation method on the surface of the agar plate. Then we test the pathogenicity according to Koch postulates and determined the type of pathogenic bacteria by morphology and molecular biology. The results showed， at first the pathogenic bacterial colonies were white or gray-white at the initial stage， and all the pathogenic bacterial colonies were reddish-brown at the later stage. The center of the colonies was orange-red， pink， or white with bulges， and the edges were gray-white in an irregular shape. The conidia was spherical， nearly spherical or oval， with a septum in the middle， mostly in clusters， about 20 μm × 5 μm. Combined with molecular biology result， the pathogen was identified as Epicoccum sorghinum. The E. sorghinum was isolated from soybean for the first time， and it was found that the pathogen was highly pathogenic after re-inoculation.
参考文献/References:
［1］孙长征. 大豆灰斑病发生规律及防控技术研究[D]. 哈尔滨:黑龙江大学， 2010. (SUN C Z. Study on occurrence regularity and control technology of soybean gray spot disease[D]. Harbin: Heilongjiang University， 2010.)[2]韩新华， 许艳丽， 李春杰， 等. 大豆灰斑病的发生与综合防治[J]. 大豆科技， 2007(3): 14-16. (HAN X H， XU Y L， LI C J， et al. Occurrence and management of frog-eye leaf spot in soybean[J]. Soybean Science & Technology， 2007(3): 14-16.)[3]许伟民， 谢昀烨， 王春伟， 等. 北五味子黑斑病的药剂防治[J]. 吉林农业大学学报， 2013， 35(5): 520-529. (XU W M， XIE Y Y， WANG C W， et al. Chemical control alternaria leaf spot of schisandra caused by Alternaria tenuissima[J]. Journal of Jilin Agricultural University， 2013， 35(5): 520-529.)[4]吉丽， 田磊， 卢宝慧， 等. 大豆灰霉病和黑斑病拮抗菌株的筛选和鉴定[J]. 土壤与作物， 2020， 9(1): 31-39. (JI L， TIAN L， LU B H， et al. Screening and identification of antagonistic strains of soybean Botrytis cinerea and Alternaria tenuissima[J]. Soils and Crops， 2020， 9(1): 31-39.)[5]HARTMAN G L， 王探应， 陈雯. 大豆红叶斑病[J]. 植物检疫， 1989(1): 65-68. (HARTMAN G L， WANG T Y， CHEN W. Red leaf blotch of soybean[J]. Plant Quarantine， 1989(1): 65-68.)[6]常雪艳， 严进. 大豆红叶斑病[J]. 植物检疫， 2009， 23(1): 4. (CHANG X Y， YAN J. Red leaf blotch of soybean[J]. Plant Quarantine， 2009， 23(1): 4.)[7]靳学慧, 吴兴泉, 马汇泉, 等. 大豆壳针孢菌生物学特性的研究[J]. 植物保护学报, 1996(3): 285-286. (JIN X H, WU X Q, MA H Q, et al. Studies on the biological characteristics of Septoria glycines hemmi[J]. Journal of Plant Protection, 1996(3): 285-286.)[8]方中达. 植病研究方法[M]. 北京:中国农业出版社， 1998. (FANG Z D. Research methods of plant diseases[M]. Beijing: China Agriculture Press， 1998.)[9]李华义, 何运转, 张艳杰, 等. 掌叶半夏疫病病原菌的分离与鉴定[J]. 河北农业大学学报, 2016, 39(4): 68-72. (LI H Y, HE Y Z, ZHANG Y J, et al. Isolation and identification of blight disease on Pinellia pedatisecta[J]. Journal of Agricultural University of Hebei, 2016, 39(4): 68-72. [10]丛丽丽， 康俊梅， 张铁军， 等. 苜蓿镰刀菌根腐病病原菌的分离鉴定与致病性分析[J]. 草地学报， 2017， 25(4): 857-865. (CONG L L， KANG J M， ZHANG T J， et al. Identification and pathogenicity test of pathogenic Fusarium of alfafa root rot[J]. Acta Agrestia Sinica， 2017， 25(4): 857-865.)[11]ANALIA E P，〖KG(0.3mm〗 MARA V M. First report of Phoma sorghina (Sacc.) Boerema Dorenbosch & van Kest on wheat leaves (Triticum aestivum L.) in Argentina[J]. Mycopathologia， 2005， 159(1): 75-78.[12]PAOUTOV S. Genetic variation of Phoma sorghina isolates from Southern Africa and Texas[J]. Folia Microbiologica， 2009， 54(3): 217-229.[13]OLIVEIRA R C， DAVENPORT K W， HOVDE B， et al. Draft genome sequence of sorghum grain mold fungus Epicoccum sorghinum， a producer of tenuazonic acid[J]. Genome Announcements， 2017， 5(4): e01495-16.[14]朱香. Epicoccum sorghinum的分离及多基因转玉米株系的抗性鉴定[D]. 武汉: 华中农业大学, 2018. (ZHU X. Isolation of Epicoccum sorghinum and resistance identification of multi-transgenes maize lines[D]. Wuhan: Huazhong Agricultural University, 2018.)[15]张国辉， 李向阳， 顾焕先， 等. 黔东南州红心猕猴桃叶尖干枯病病原鉴定[J]. 中国农学通报， 2021， 37(3): 145-149. (ZHANG G H， LI X Y， GU H X， et al. Identification of pathogens of dry blight disease in leaf tip of red-fleshed kiwi fruit in Qiandongnan prefecture[J]. Chinese Agricultural Science Bulletin， 2021， 37(3):145-149.)[16]ROHIK T， HUDEC K. Fungal infection of malt barley kernels in Slovak Republic[J]. Plant Protection Science， 2007， 43(3): 86-93.[17]LIU L M， ZHAO Y， ZHANG Y L， et al. First report of leaf spot disease on rice caused by Epicoccum sorghinum in China[J]. Plant Disease， 2020， 104(10):2735.[18]DU Y X， XU Y， JIANG J Y， et al. First report of Epicoccum sorghinum causing leaf spot on atractylodes lancea in Hubei Province， China[J]. Plant Disease， 2020， 104(11):3082.[19]黄鈜琳, 尹桥秀, 江仕龙, 等. 茶叶斑病病原菌高粱附球菌的鉴定及生物学特性[J]. 热带作物学报, 2021, 42(11): 3269-3277. (HUANG H L, YIN Q X, JIANG S L, et al. Identification and biological characteristics of Epicoccum sorghinum, pathogen of Tea Leaf Spot[J]. Chinese Journal of Tropical Crops, 2021, 42(11): 3269-3277.)[20]曾慧兰， 卢其能， 曾鹏远， 等. 百合叶尖干枯病病原菌的鉴定， 生物学特性及其对杀菌剂的敏感性研究[J]. 园艺学报， 2018， 45(12): 2407-2416. (ZENG H L， LU Q N， ZENG P Y， et al. Identification， biological characteristics and sensitivity of the causal pathogen inducing leaf dieback on lily[J]. Acta Horticulturae Sinica， 2018， 45(12): 2407-2416.)[21]FU R T， CHEN C， WANG J， et al. First report of Epicoccum sorghinum causing leaf spot on Paris polyphylla in China[J]. Plant Disease， 2019， 103(6): 1426.[22]CHEN X L， WANG Y H， LUO T. First report of leaf spot caused by Phoma sorghina on Oxalis debilis in China[J]. Plant Disease， 2017， 101(6): 1047.[23]YU L， SHE X M， LAN G B， et al. First report of leaf spot caused by Epicoccum sorghinum on Chinese flowering cabbage (Brassica parachinensis) in China[J]. Plant Disease， 2019， 103(11): 2966.[24]彭成彬, 王泽榕, 阮俊峰, 等. 柳叶蜡梅真菌病害分离鉴定及生物学特性[J]. 福建农业学报, 2021, 36(4): 457-463. (PENG C B, WANG Z R, RUAN J F, et al. Identification and biology of leaf spot pathogen on Chimonanthus salicifolius[J]. Fujian Journal of Agricultural Sciences, 2021, 36(4): 457-463.)[25] SAXENA S C. Identification of resistance sources to banded leaf and sheath blight (Rhizoctonia solani f. sp. sasakii) in maize[J]. Indian Phytopathology， 2013， 60(2): 162-166.
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(03):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(03):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(03):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(03):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(03):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
收稿日期：2021-10-27