(XIE Jiao,GAO Guo-rong,ZHAO Dun-hou,et al.Promoting Effects of Adding Biological Agents on Soybean Seedlings Growth Under Low Temperature[J].Soybean Science,2021,40(04):517-521.[doi:10.11861/j.issn.1000-9841.2021.04.0517]
添加生物菌剂对低温条件下大豆幼苗生长的促进作用
- Title:
- Promoting Effects of Adding Biological Agents on Soybean Seedlings Growth Under Low Temperature
- Keywords:
- Soybean; Biological agent; Low temperature; Physiology; Growth
- 文献标志码:
- A
- 摘要:
- 为探究低温条件下生物菌剂对大豆苗期生长的影响，本研究对大豆进行温度和生物菌剂的双重处理，测定其株高、株幅、叶片相对含水量、叶绿素含量、叶面积及酶活性指标，分析大豆生理生化差异。结果表明: 在低温条件下添加生物菌剂能够优化大豆幼苗的各项形态指标，添加生物菌剂后大豆幼苗的株高、株幅、叶面积和含水量比正常条件下分别提高了1.86 cm、 2 cm、 169.6 mm2和25.09%。在低温条件下添加生物菌剂后叶绿素含量下降并且各种抗氧化酶活性发生不同程度的变化。低温下添加菌剂的大豆叶绿素含量比未加菌剂处理低0.01 mg·g-1。在低温条件下，与未加菌剂处理相比，添加菌剂的SOD降低5.73 μmol·g-1·min-1，POD和CAT活性分别提高了159.47和1.31 μmol·g-1·min-1。投影寻踪综合评价模型的评价结果进一步说明低温条件下添加生物菌剂对大豆苗期生长发育产生积极的影响。
- Abstract:
- In order to explore the effects of biological agents on the growth of soybean seedlings under low temperature, the plant height, plant width, relative water content of leaves, chlorophyll content, leaf area and enzyme activity of soybean were measured by dual treatment of temperature and biological agents. The results showed that the morphological indexes of soybean seedlings could be improved by adding biological agent under low temperature. The plant height, plant width, leaf area and water content of soybean seedlings increased by 1.86 cm, 2 cm, 169.6 mm2 and 25.09% respectively compared with those under normal conditions. Under low temperature, the content of chlorophyll decreased and the activities of antioxidant enzymes changed in varying degrees, and the chlorophyll content of soybean with inoculant was 0.01 mg·g-1 lower than that without inoculant, and the activities of SOD, POD and CAT were decreased by 5.73 μmol·g-1·min-1, increased by 159.47 and 1.31 μmol·g-1·min-1, respectively. The comprehensive evaluation results of projection pursuit comprehensive evaluation model further showed that the addition of biological agents under low temperature had a positive effect on the growth and development of soybean seedlings.
参考文献/References:
［1］潘文华,许世卫. 黑龙江省大豆产业困境与差异化发展战略［J］. 农业经济问题, 2014, 35(2): 26-33. (Pan W H, Xu S W. Dilemma and differentiated development strategy of soybean industry in Heilongjiang Province［J］. Issues in Agricultural Economy, 2014, 35(2): 26-33.)［2］张永芳,钱肖娜,王润梅,等.不同大豆材料的抗旱性鉴定及耐旱品种筛选［J］.作物杂志, 2019(5): 41-45. (Zhang Y F, Qian X N, Wang R M, et al. Identification of drought resistance of different soybean materials and screening of drought tolerant varieties［J］. Crops, 2019(5): 41-45.)［3］刘志娟,杨晓光,王文峰,等.气候变化背景下我国东北三省农业气候资源变化特征［J］.应用生态学报, 2009, 20(9): 2199-2206. (Liu Z J, Yang X G, Wang W F, et al. Characteristics of agricultural climate resources in three provinces of northeast china under global climate change［J］. Chinese Journal of Applied Ecology, 2009, 20(9): 2199-2206.)［4］Mullet J E, Whitsitt M S. Plant cellular responses to water deficit［J］. Plant Growth Regulation, 1996, 20(2): 119-124.［5］刘珍环, 唐鹏钦, 范玲玲, 等. 1980—2010年东北地区种植结构时空变化特征［J］. 中国农业科学, 2016, 49(21): 4107-4119. (Liu Z H, Tang P Q, Fan L L, et al. Spatio-temporal changes of cropping types in northeast China during 1980-2010［J］. Scientia Agricultura Sinica, 2016, 49(21): 4107-4119.)［6］盖志佳, 张敬涛, 刘婧琦, 等. 耐低温大豆品种(系)的筛选与研究［J］.中国种业, 2018(6): 57-60. (Gai Z J, Zhang J T, Liu J Q, et al. Screening and study on low temperature tolerant soybean varieties(lines)［J］. China Seed Industry, 2018(6):57-60.)［7］张大伟,杜翔宇,刘春燕,等.低温胁迫对大豆萌发期生理指标的影响［J］.大豆科学,2010,29(2):228-232. (Zhang D W, Du X Y, Liu C Y, et al. Effect of low-temperature stress on physiological indexes of soybean at germination stage［J］. Soybean Science,2010,29(2):228-232.)［8］张思河,王萍,马淑英,等.三个熟期类型大豆种子萌发进程中耐冷性的比较［J］.大豆科学,2000,19(3): 218-222. (Zhang S H, Wang P, Ma S Y, et al. Comparative study on the cold tolerance of three maturity type soybean seeds during the course of germination［J］. Soybean Science,2000,19(3):218-222.)［9］郝晶,张立军,谢甫绨.低温对大豆不同耐冷性中萌发期保护酶活性的影响［J］.大豆科学,2007,26(2):171-175. (Hao J, Zhang L J, Xie F T. Effects of the low temperature on defense enzyme activities of different chilling tolerant soybean cultivars during the germination［J］. Soybean Science,2007, 26(2): 171-175.)［10］鲁杰,刘宝忠,周传远,等.生物有机菌肥对水稻产量及稻米品质的影响［J］.中国农学通报, 2009, 25(6): 146-150. (Lu J, Liu B Z, Zhou C Y, et al. Effect of biological manure fertilizer on yield and quality of rice［J］. Chinese Agricultural Science Bulletin,2009,25(6):146-150.)［11］朱云娜,刘建国.生物菌肥在玉米栽培上的效果［J］.安徽农业科学,2013,41(28):11354-11356. (Zhu Y N, Liu J G. Effects of biological bacterium manure in maize cultivation［J］. Journal of Anhui Agricultural Sciences,2013,41(28):11354-11356.)［12］崔曾杰,耿艳秋,范丽丽,等.生物菌肥对盐碱地水稻生长发育及产量的影响［J］.吉林农业科学,2013,38(5):32-35. (Cui Z J, Geng Y Q, Fan L L, et al. Effects of bacterial manure on the growth and yield of rice growing in saline-alkali land［J］. Journal of Northeast Agricultural Sciences,2013,38(5):32-35.)［13］毛家伟,张翔,李亮,等.施用生物菌剂对烤烟叶片生理特征及钾、氯含量的影响［J］.干旱地区农业研究,2020,38(3):181-187. (Mao J W, Zhang X, Li L, et al. Effects of biological agent application on leaf physiological characteristics, potassium and chlorine contents of flue-cured tobacco［J］. Agricultural Research in the Arid Areas,2020,38(3):181-187.)［14］侯立刚,刘会芳,田健,等.不同菌剂浓度对低温处理下水稻种子萌发能力的影响［J］.种子,2021,40(3):87-96. (Hou L G, Liu H F, Tian J, et al. Effects of different concentrations of bacterial agents on the germination ability of rice seeds under low temperature treatment［J］. Seed,2021,40(3):87-96.)［15］乌兰,夏尔瓦古丽·吐尔逊江,戴爱梅.两种生物菌剂对棉花低温抗逆增产的田间试验［J］.植物医生,2017,30(6):32-34. (Wu L, Sharvaguli T, Dai A M. Field experiment of two biological agents on low temperature stress resistance and yield increase of cotton［J］. Plant Doctor, 2017, 30(6): 32-34.)［16］刘佳琪,关法春,黄风兰,等.低温条件下生物菌肥对蓖麻生长影响的研究［J］.辽宁林业科技,2020(6):19-22. (Liu J Q, Guan F C, Huang F L, et al. The effect of biological bacterial 〖JP4〗fertilizer on the growth of Ricinus communis under low temperature［J］. Liaoning Forestry Science and Technology, 2020(6): 19-22.)［17］隋世江,安景文,娄春荣,等.微生物菌剂对大豆产量和根瘤数的影响［J］.辽宁农业科学,2010(6):49-50. (Sui S J, An J W, Lou C R, et al. Effects of microbial agents on soybean yield and nodule number［J］. Liaoning Agricultural Sciences,2010(6): 49-50.)［18］刘文志,王北兰,李鹏,等.生物制剂对大豆根腐病菌的抑制及对大豆幼苗生长的影响［J］.现代化农业,2010(12):21-24. (Liu W Z, Wang B L, Li P, et al. Inhibition of biological agents on soybean root rot and its effect on the growth of soybean seedlings［J］. Modernizing Agriculture,2010(12): 21-24.)［19］葛红莲,张福丽,李俐俐.复合光合菌剂对大豆幼苗生理生化特性和根际土壤酶活性的影响［J］.大豆科学, 2014, 33(5): 713-717. (Ge H L, Zhang F L, Li L L. Effects of composite photosynthetic bacteria PS11 on physiological-biochemical characteristics and rhizosphere soil enzyme activities of soybean seedling ［J］. Soybean Science, 2014, 33(5): 713-717.)［20］王学奎. 植物生理生化实验原理与技术［M］. 2版. 北京: 高等教育出版社, 2006. (Wang X K. Principles and techniques of plant physiological and biochemical experiments［M］. 2nd Edition. Beijing: Higher Education Press, 2006.)［21］李合生. 植物生理生化实验原理与技术［M］. 北京: 高等教育出版社, 2000: 134-260. (Li H S. Principles and techniques of plant physiological and biochemical experiments［M］. Beijing: Higher Education Press, 2000: 134-260.)［22］李凯峰, 姜存良, 包昌艳, 等. 生物菌肥对猕猴桃生长和生理特性的影响［J］. 中国果树, 2020(3): 72-75. (Li K F, Jiang C L, Bao C Y, et al. Effects of microbial fertilizer on growth and physiological characteristics of kiwifruit［J］. China Fruits, 2020(3): 72-75.)［23］郁万文. 银杏抗寒机理及种质资源抗寒性评定的初步研究［D］. 南京: 南京林业大学, 2008. (Yu W W. Preliminary studies on cold-resistance mechanism and identification of Ginkgo germplasm［D］. Nanjing: Nanjing Forestry University, 2008.)［24］李明玉,曹辰兴,于喜艳.低温锻炼对冷胁迫下黄瓜幼苗保护性酶的影响［J］. 西北农业学报, 2006, 15(1): 160-164. (Li M Y, Cao C X, Yu X Y. Effects of cold acclimation on protective enzymes of cucumber seedling under cold stress［J］. Acta Agriculturae Boreali-occidentalis Sinica, 2006, 15(1): 160-164.)［25］Silin S N, Guy R D, Lavender D P. Mefluidide-induced drought resistance in seedings of three cenifer species［J］. Canadian Journal of Botany, 1993, 71(8): 1087-1092.［26］崔帅, 赵兰勇, 李承水, 等. 卫矛属3种常绿阔叶树木抗寒性研究［J］. 中国农学通报, 2012, 28(7): 17-20. (Cui S, Zhao L Y, Li C S, et al. Research on cold resistance in three evergreen broadleaf trees of Euonymus［J］. Chinese Agricultural Science Bulletin, 2012, 28(7): 17-20.)［27］卢芳, 朱鸿菊, 秦登. 徐州地区8种常绿阔叶乔木抗寒性研究［J］. 江苏农业科学, 2011, 39(5): 212-216. (Lu F, Zhu H J, Qin D. Study on cold resistance of 8 evergreen broad-leaved trees in Xuzhou［J］. Jiangsu Agricultural Sciences, 2011, 39(5): 212-216.)［28］杜有新,何春林,张乐华,等.庐山若干常绿树种对冬季低温的生理生化响应［J］.生态环境学报,2014,23(6):945-949. (Du Y X, He C L, Zhang L H, et al. Physiochemical response of several evergreen trees to low temperature stress in winter in Lushan［J］. Ecology and Environmental Sciences, 2014, 23(6): 945-949.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
收稿日期：2021-02-19