ZHANG Jun,YIN Jun-yi,HE Jia-qi,et al.Abiotic Stress Induced Expression and Promoter Prediction of GmDof4 and GmDof11 Gene in Soybean[J].Soybean Science,2021,40(02):195-220.[doi:10.11861/j.issn.1000-9841.2021.02.0195]
大豆GmDof4和GmDof11基因的非生物胁迫诱导表达及启动子分析
- Title:
- Abiotic Stress Induced Expression and Promoter Prediction of GmDof4 and GmDof11 Gene in Soybean
- Keywords:
- Soybean; Dof transcription factor; Abiotic stress; Promoter; cis-element
- 文献标志码:
- A
- 摘要:
- Dof家族是典型的植物特异性锌指转录因子家族，在植物中可以对非生物胁迫产生应答。为初步研究大豆Dof家族主要转录因子编码基因GmDof4和GmDof11在大豆抗非生物胁迫过程中的作用及调控原理，本研究通过实时荧光定量PCR检测大豆幼苗中GmDof4和GmDof11基因在非生物胁迫下的表达情况，并使用PlantCARE在线数据库分析两个基因上游启动子的作用元件。结果显示：GmDof4在干旱、高盐、高温和低温胁迫下表达量升高；GmDof11在干旱、高盐和低温胁迫下表达量升高，在高温胁迫下表达量下降。启动子顺式作用元件预测结果显示，GmDof4启动子中含有1个厌氧诱导元件、1个低温响应元件和1个MYB转录因子结合位点；GmDof11启动子中含有3个厌氧诱导元件、2个低温响应元件、1个防御和胁迫响应元件和1个MYB转录因子结合位点。此外，它们的启动子序列中还含有脱落酸响应元件、茉莉酸甲酯响应元件、赤霉素响应元件以及生长素响应元件。结果说明GmDof4和GmDof11的启动子区域含有逆境相关顺式作用元件，能够参与大豆对非生物胁迫的应答。
- Abstract:
- Dof family is a typical transcription factors family of plant-specific zinc finger that can respond to abiotic stress in plants. In order to preliminarily study the role and regulation principle of transcription factor coding gene GmDof4 and GmDof11, we studied the major transcription factors of soybean Dof family in abiotic stress resistance. We detected the expressions of GmDof4 and GmDof11 in soybean seedlings under abiotic stress by real-time fluorescence quantitative PCR, and analyzed the cis-elements in the promoters of the two genes with PlantCARE online database. The result showed that the expression of GmDof4 increased under drought, high salinity, high temperature and low temperature stresses. The expression of GmDof11 increased under drought, high salinity and low temperature stresses, but decreased under high temperature stress. The prediction result of promoter cis-elements showed that the GmDof4 promoter contained one anaerobic induction element (ARE), one low temperature responsive element and one MYB transcription factor binding site. The GmDof11 promoter contained three AREs, two LTRs, one defense and stress responsive element (TC-rich repeats) and one MYB transcription factor binding site. In addition, their promoter sequences also contained abscisic acid responsive elements, MeJA responsive elements, gibberellin responsive elements and auxin responsive elements. These results indicated that the promoter regions of GmDof4 and GmDof11 contained stress-related cis-elements, and GmDof4 and GmDof11 were involved in response to abiotic stress in soybean.Keywords:
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备注/Memo
收稿日期：2020-12-04