LIU Wei,ZHANG Yan-wei,WANG Yu-bin,et al.Identification of Waterlogging Responsive NAC Genes in Soybean and Cloning and Hormone Responsive Analysis of GmNAC038[J].Soybean Science,2021,40(02):159-167.[doi:10.11861/j.issn.1000-9841.2021.02.0159]
大豆涝渍响应NAC基因的鉴定及GmNAC038的克隆和激素应答分析
- Title:
- Identification of Waterlogging Responsive NAC Genes in Soybean and Cloning and Hormone Responsive Analysis of GmNAC038
- Keywords:
- Soybean; Waterlogging stress; NAC transcription factors; GmNAC038; Clone; cis-element
- 文献标志码:
- A
- 摘要:
- NAC是植物特有的转录因子，参与多种非生物胁迫的响应。为了研究NAC转录因子在大豆涝渍胁迫中的响应和调控作用，利用前期获得的耐涝品种齐黄34在涝渍胁迫下的转录组数据，通过分析大豆全基因组内180个NAC基因的表达情况，鉴定出45个持续响应涝渍胁迫的大豆NAC基因。利用PlantCARE在线软件分析发现这些基因的启动子中均至少含有1种逆境或激素应答元件，推测它们可能与大豆的非生物胁迫应答相关。同时，对涝渍胁迫响应程度最大的大豆NAC基因GmNAC038进行克隆和分析。利用在线分析软件CDD分析发现该基因编码的蛋白质含有保守的NAM结构域。qRT-PCR表明GmNAC038不仅受到涝渍胁迫的强烈诱导，还受到脱落酸、乙烯和茉莉酸甲酯的正向调控。研究结果可为今后探索NAC基因在大豆涝渍胁迫响应中的功能提供分子基础，为培育耐涝大豆品种提供基因资源。
- Abstract:
- As a plant-specific transcription factor, NAC has been reported to be involved in multiple abiotic stresses. In order to study on the response and regulation of NAC transcription factors to waterlogging stress in soybean, we analyzed the expression of 180 NAC genes of soybean based on a transcriptome data of the waterlogging resistant soybean variety Qihuang 34 suffered from submergence treatment. 45 continually waterlogging responsive NAC genes were identified. By using the PlantCARE software, we found that all of the 45 NAC genes possessed at least 1 stress or hormone-responsive cis-element, suggesting that these genes might be involved in abiotic stress responses. We then cloned GmNAC038, a NAC gene which exhibited the most sensitive to the waterlogging stress. By using the online software CDD, GmNAC038 was found to contain a conserved NAM domain. Real-time quantitative PCR showed that GmNAC038 was not only strongly induced by waterlogging stress, but was also up-regulated by ABA, ethylene and methyl jasmonate. This study provides a molecular basis for further research on the function of NAC genes in soybean waterlogging stress responses, and will provide genetic resources for the flooding-tolerance cultivar breeding of soybean.
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备注/Memo
收稿日期：2020-12-21