CAI Han,ZHAO Lin,SHEN Ying,et al.Molecular Marker Screening and Resistance Identification of Soybean Germplasem to SMV Strain SC3[J].Soybean Science,2021,40(01):1-10.[doi:10.11861/j.issn.1000-9841.2021.01.0001]
春大豆鼓粒期翻叶对叶片生理活性的影响春大豆鼓粒期翻叶对叶片生理活性的影响
- Title:
- Molecular Marker Screening and Resistance Identification of Soybean Germplasem to SMV Strain SC3
- Keywords:
- Soybean mosaic virus; Molecular markers; SSR; CH0211; Resistance identification; Coincidence rate of disease resistance phenotypic
- 文献标志码:
- A
- 摘要:
- 为揭示鼓粒期大豆冠层叶片翻转后对叶片衰老和光合速率的影响规律。2018—2019年，以吉育60、新大豆27号为材料，田间研究了鼓粒期人工模拟自然翻叶（倒4叶）对叶片净光合速率、抗氧化酶活性、丙二醛含量、可溶性蛋白质和可溶性糖含量的影响规律。结果表明，翻叶0～10 d叶片SOD、POD、CAT酶活性高于对照，翻叶10~20 d低于对照；翻叶后叶片丙二醛含量较对照叶片持续增加，叶片可溶性蛋白质含量降低；翻叶0~20 d叶片近轴面净光合速率较对照叶近轴面降低33.3%～41.5%，叶片可溶性糖含量大幅降低。推测翻叶降低叶片光合速率是叶片远轴面光合速率低于近轴面和翻叶加速叶片衰老进程的共同作用结果。在高温和强光条件下，翻转叶片较对照叶片加速衰老是叶片膜脂过氧化进程加速的结果。
- Abstract:
- To further search for molecular markers closely linked to the resistance genes (Rsc3) of soybean mosaic virus (SMV) prevalent strain SC3, providing an accurate and rapid method for breeding resistant cultivars and promoting the SMV resistance genes mining. In the present study, 30 SSR markers newly designed near the SMV resistance site and 50 SSR markers known to be linked to Rsc3 were preliminary screened via 96 soybean cultivars(lines), and the resistance of288 newly bred soybean varieties(lines) from 2016 to 2019 in China were identified combining with genotype, phenotype and serological identification. The result showed that, one newly developed SSR marker CH0211 was initially screened, and the coincidence rate with the resistance phenotypic selection of soybean varieties was 87.8%. Then, 288 soybean cultivars(lines) were further detected and analyzed by using this marker. With the combination of artificial inoculation phenotype and serological identification, the coincidence rate of disease resistance phenotype and genotype was 80.56%. In this study, 158 soybean cultivars with disease resistance to SC3 were identified from 288 soybean cultivars(lines), accounting for 54.86% of the total tested materials, and the molecular bands of 144 soybean cultivars(lines) were identified that were consistent with the type of disease-resistant cultivars(lines). Totally，the phenotypes of 116 cultivars(lines) were consistent with the resistance molecular bands, including PI96983, Qihuang 34, Fendou 104, and Shanning 28. All in all, we developed and identified a molecular marker CH0211 closely linked to Rsc3. This marker can be effectively used in germplasm selection of soybean cultivars resistant to SMV SC3 and accelerate the breeding process of soybean resistance to SMV.
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备注/Memo
国家重点研发计划（2017YFD0101504）；国家自然科学基金（31671718）；现代农业产业技术体系建设专项（CARS-04）；江苏省现代作物生产协同创新（JCIC-MCP）；现代作物生产省部共建协同创新中心（CIC-MCP）。