ZHENG Li-ping,ZHANG Yun-feng,JIANG Hong-wei,et al.Comprehensively Evaluation and Screening on Low Temperature Tolerance of Soybean Germplasm Resources at Bud Stage[J].Soybean Science,2020,39(06):833-847.[doi:10.11861/j.issn.1000-9841.2020.06.0833]
大豆种质资源芽期耐低温综合评价及筛选
- Title:
- Comprehensively Evaluation and Screening on Low Temperature Tolerance of Soybean Germplasm Resources at Bud Stage
- Keywords:
- Soybean; Bud stage; Low temperature stress; Germination; Membership function method; Cluster analysis
- 文献标志码:
- A
- 摘要:
- 种子活力高低直接影响大豆出苗质量，进而影响大豆的高产与稳产。为应对近年来东北地区低温胁迫对大豆种子活力的影响日趋严重的问题，筛选低温胁迫下高活力的种质资源，本研究鉴定335份大豆资源芽期的耐低温性，分别设置6 ℃和20 ℃处理，测定发芽势、发芽率、发芽指数及各指标的差异性和相关性，利用隶属函数法对这些资源进行综合评价。结果表明：6 ℃低温处理降低大豆种子的发芽势、发芽率和发芽指数，且各指标在品种间差异显著（P＜0.01）；相关性分析结果表明发芽势、发芽率和发芽指数彼此间在0.01水平上呈显著正相关，且相关系数均大于0.8；隶属函数综合评价结果表明，335份大豆资源耐低温性存在不同程度差异；聚类分析可将品种分为3类：合农97、吉林小粒豆CK和黑农69等17个品种耐低温性较强；合农76、SB8842和垦鉴豆43等161个品种耐低温性次之；公05163-3、华庆豆103和龙黑大豆1号等157个品种耐低温性差。研究结果将为耐低温大豆品种的选育提供材料基础和理论依据。
- Abstract:
- Seed vigor directly affects the quality of soybean seedling, and then affects the high and stable yield of soybean. In order to deal with the problem of low temperature stress on soybean seed vigor in northeast China in recent years, and screen the germplasm resources with high vigor under low temperature stress, in this study, the low temperature resistance of the budding stage of 335 soybean resources were identified and treated at 6 ℃ and 20 ℃ respectively. The germination energy, germination rate and germination index were determined, and the difference and correlation of indexes were analyzed. Then, the membership function method was used to comprehensively evaluate these resources. The results showed that: 6 ℃ low temperature treatment reduced the germination energy, germination rate and germination index of soybean seeds, each index was significantly different among different varieties. The results of correlation analysis showed that the germination energy, germination rate and germination index were significantly positively correlated at the level of 0.01, and the correlation number was greater than 0.8. The comprehensive evaluation of membership function and cluster analysis results showed that 335 soybean varieties had significant differences. The cluster analysis of 335 soybean resources could be divided into three categories: 17 varieties, such as Henong 97, Jilinxiaolidou CK and Heinong 69, had strong low temperature tolerance, 161 varieties, such as Henong 76, sb8842 and Kenjiandou 43 were the second, 157 varieties such as Gong 05163-3, Huaqingdou 103 and Longheidadou 1 had poor low temperature tolerance. These results would provide material and theoretical basis for breeding of low temperature resistant soybean varieties.
参考文献/References:
［1］张永芳, 钱肖娜, 王润梅, 等. 不同大豆材料的抗旱性鉴定及耐旱品种筛选［J］. 作物杂志, 2019(5): 41-45. (Zhang Y F, Qian X N, Wang R M, et al. Identification of drought resistance of different soybean materials and selection of drought-tolerant varieties［J］. Journal of Crops, 2019(5): 41-45.)［2］刘志娟, 杨晓, 王文峰, 等. 气候变化背景下我国东北三省农业气候资源变化特征［J］. 应用生态学报, 2009, 20(9): 2199-2206. ( Liu Z J, Yang X, Wang W F, et al. Change characteristics of agricultural climate resources in three northeastern provinces of China under the background of climate change［J］. Chinese Journal of Applied Ecology, 2009, 20(9): 2199-2206.)［3］Yamaguchi-Shinozaki K,Shinozaki K. Characterization of the expression of a desiccation-responsive rd29A gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants［J］. Molecular and General Genetics, 1993, 236(2-3): 331-340.［4］Mullet J E, Whitsitt M S. Plant cellular responses to water deficit［J］. Plant Growth Regulation, 1996, 20(2): 119-124.［5］刘珍环, 唐鹏钦, 范玲玲, 等. 1980—2010年东北地区种植结构时空变化特征［J］. 中国农业科学, 2016, 49(21): 4107-4119. (Liu Z H, Tang P Q, Fan L L, et al. Spatial and temporal variation characteristics of planting structure in northeast China from 1980 to 2010［J］. Scientia Agricultura Sinica, 2016, 49(21): 4107-4119.)［6］王州飞. 盐和低温胁迫下水稻种子萌发的遗传研究［D］. 南京: 南京农业大学, 2010. (Wang Z F. Genetic study on rice seed germination under salt and low temperature stress［D］. Nanjing: Nanjing Agricultural University, 2010.)［7］Andaya V C,Tai T H. Fine mapping of the 〖STBX〗qCTS4〖STBZ〗 locus associated with seedling cold tolerance in rice (Oryza sativa L.)［J］. Molecular Breeding, 2007(4): 349-358.［8］Zeng Y, Yu J, Cang J, et al. Detection of sugar accumulation and expression levels of correlative key enzymes in winter wheat (Triticum aestivum) at low temperatures［J］. Bioscience Biotechnology and Biochemistry, 2011, 75(4): 681-687.［9］Qi H, Hua L, Zhao L, et al. Carbohydrate metabolism in tomato(Lycopersicon esculentum Mill. ) seedlings and yield and fruit quality as affected by low night temperature and subsequent recovery［J］. African Journal of Biotechnology, 2013, 10 (30): 5743-5749.〖ZK)〗［10］〖ZK(#〗Liu Y F, Qi M F, Li T L. Photosynthesis, photoinhibition, and antioxidant system in tomato leaves stressed by low night temperature and their subsequent recovery［J］. Plant Science, 2012, 196(11): 8-17.［11］Mizoi J,Ohori T, Moriwaki T, et al. GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT- BINDING PROTEIN2-type transcription factor in soybean, is post translationally regulated 〖JP4〗and mediates dehydration-responsive element-dependent gene expression［J］. Plant Physiology, 2013, 161(1), 346-361.［12］张大伟, 杜翔宇, 刘春燕, 等. 低温胁迫对大豆萌发期生理指标的影响［J］. 大豆科学, 2010, 29(2): 229-232. (Zhang D W, Du X Y, Liu C Y, et al. Effect of low temperature stress on physiological indexes of soybean germination［J］. Soybean Science, 2010, 29(2): 229-232.)［13］单彩云. 大豆耐低温资源筛选及蛋白质组学研究［D］. 哈尔滨: 东北农业大学, 2008. (Shan C Y. Selection of soybean low-temperature resistant resources and proteomics research［D］. Harbin: Northeast Agricultural University, 2008.)［14］胡俊杰, 张古文, 胡齐赞, 等. 低温胁迫对菜用大豆生长、叶片活性氧及多胺代谢的影响［J］. 浙江农业学报, 2011, 23(6): 1113-1118. (Hu J J, Zhang G W, Hu Q Z, et al. Effects of low temperature stress on the growth, leaf reactive oxygen species and polyamine metabolism of vegetable soybean［J］. Acta Agriculturae Zhejiang, 2011, 23(6): 1113-1118.)［15］宰学明，吴国荣.低温预处理对大豆萌芽活力及其活性氧代谢的影响［J］. 大豆科学, 2001, 20(3): 163-166. (Zai X M, Wu G R. Effects of low temperature pretreatment on germination activity and reactive oxygen metabolism of soybean［J］. Soybean Science, 2001, 20(3): 163-166.)［16］宋剑陶, 顾增辉. 大豆抗冷性生理生化指标的筛选［J］ . 中国农业科学, 1992, 25(4): 15-23. (Song J T, Gu Z H. Screening of physiological and biochemical indexes of soybean cold resistance［J］. Scientia Agricultura Sinica, 1992, 25(4): 15-23.)［17］蒋洪蔚, 李灿东, 刘春燕, 等. 大豆导入系群体芽期耐低温位点的基因型分析及QTL定位［J］. 作物学报, 2009, 35(7): 1268-1273. (Jiang H W, Li C D, Liu C Y, et al. Genotype analysis and QTL mapping of low temperature tolerance sites at bud stage of imported soybean populations［J］. Acta Agronomica Sinica, 2009, 35(7): 1268-1273.)［18］胡国玉, 赵晋铭, 周斌, 等. 大豆耐低温出苗的遗传分析与分子标记［J］. 大豆科学, 2008, 27(6): 905-910. (Hu G Y, Zhao J M, Zhou B, et al. Genetic analysis and molecular markers of low temperature resistant seedling emergence of soybean［J］. Soybean Science, 2008, 27(6): 905-910.)［19］郝晶, 张立军, 谢甫绨. 低温对大豆不同耐冷性中萌发期保护酶活性的影响［J］. 大豆科学, 2007, 26(2): 171-175. (Hao J, Zhang L J, Xie F T. Effect of low temperature on the activity of protective enzyme during germination of soybean with different cold tolerance［J］. Soybean Science, 2007, 26(2):171-175.)［20］石德成, 殷立娟. 盐(NaCl)与碱(Na2CO3)对星星草胁迫作用的差异［J］. 植物学报, 1993, 35(2): 144-149. (Shi D C, Yin L J. Differences in stress effects of NaCl and Na2CO3 on Herba sinensis［J］. Journal of Integrative Plant Biology, 1993, 35(2):144-149.)［21］祈旭升, 王兴荣, 许军, 等. 胡麻种质资源成株期抗旱性评价［J］. 中国农业科学, 2010, 43(15): 3076-3087. (Qi X S, Wang X R, Xu J, et al. Evaluation of drought resistance of flax germplasm resources in plant growth stage［J］. Scientia Agricultura Sinica, 2010, 43(15): 3076-3087.)［22］Hodges D M, Andrews C J, Johnson D A, et al. Sensitivity of maize hybrids to chilling and their combining abilities at two developmental stages［J］. Crop Science, 1997, 37(3): 850-856.［23］唐桂香, 汪自强. 春播和秋播对南方春大豆种子活力的影响［J］. 作物学报, 1998, 24(2): 243-247. (Tang G X, Wang Z Q. Effects of spring and autumn sowing on seed vigor of southern spring soybean［J］. Acta Agronomica Sinica, 1998, 24 (2): 243-247.)［24］顾增辉, 宋剑陶. 大豆抗冷性生理生化指标的筛选［J］. 中国农业科学, 1992, 11(4)：15-23. (Gu Z H, Song J T. Screening of physiological and biochemical indexes of soybean cold resistance ［J］. Scientia Agricultura Sinica, 1992, 11(4): 15-23.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]牛远,杨修艳,戴存凤,等.大豆芽期和苗期耐盐性评价指标筛选[J].大豆科学,2018,37(02):215.[doi:10.11861/j.issn.1000-9841.2018.02.0215]
NIU Yuan,YANG Xiu-yan,DAI Cun-feng,et al.Related Indices Selection of Soybean Salt Tolerance at Germination and Seedling Stages[J].Soybean Science,2018,37(06):215.[doi:10.11861/j.issn.1000-9841.2018.02.0215]
备注/Memo
收稿日期：2020-09-04