SANG Yong-sheng,LIU Yue,YUAN Cui-ping,et al.Systematic Identification of Fusarium Species Associated with Soybean Root Rot in Dunhua and Gongzhuling, Jilin Province[J].Soybean Science,2020,39(03):442-450.[doi:10.11861/j.issn.1000-9841.2020.03.0442]
吉林省公主岭与敦化大豆根腐病镰孢菌的系统鉴定
- Title:
- Systematic Identification of Fusarium Species Associated with Soybean Root Rot in Dunhua and Gongzhuling, Jilin Province
- Keywords:
- Soybean (Glycine max); Root rot; Fusarium; Concatenated sequence; Phylogeny
- 文献标志码:
- A
- 摘要:
- 为明确吉林省公主岭和敦化的大豆根腐病镰孢菌种类，分离93份根腐病标样的病原真菌，利用ITS、TEF1-α、RPB2基因序列比对和多基因位点系统发育分析，结合形态学鉴定方法，鉴定出104株镰孢菌，共分4种，腐皮镰孢菌占64.42%，尖镰孢菌占33.65%，轮枝镰孢菌占0.96%，层出镰孢菌占0.96%。在公主岭罹病植株上分离鉴定出57株镰孢菌，腐皮镰孢菌占91.23%，尖镰孢菌占5.27%，轮枝镰孢菌占1.75%，层出镰孢菌占1.75%；在敦化罹病植株上分离鉴定出47株镰孢菌，腐皮镰孢菌占31.91%，尖镰孢菌占68.09%。结果表明：公主岭优势病原菌为腐皮镰孢菌，而敦化优势病原菌为尖镰孢菌。致病性试验结果表明，仅有3株腐皮镰孢菌对合丰55无致病性，其余101株镰孢菌对合丰55均有不同程度的致病性。
- Abstract:
- In order to identify the fusarium species associated with soybean root rot in Dunhua and Gongzhuling,93 samples of diseased plants with typical root rot symptom were collected to isolate and identify the species of Fusarium causing the disease in Gongzhuling and Dunhua, Jilin province. Sequence alignment carried out by ITS, TEF1-α and RPB2 genes, multiple-gene phylogenetic analysis and morphology were used to identify the pathogens. 104 strains of Fusarium classified in 4 species were identified, including F. solani (64.42%), F. oxysporum (33.65%), F. verticillioides (0.96%) and F. proliferaum (0.96%). 57 out of the 104 strains of Fusarium were isolated from the diseased plants in Gongzhuling, including F. solani (91.23%), F. oxysporum (5.27%), F. verticillioides (1.75%) and F. proliferaum (1.75%). The other 47 strains were isolated from Dunhua, including F. solani (31.91%) and F. oxysporum (68.09%). The results showed F. solani and F. oxysporum were respectively the dominant pathogen in Gongzhuling and Dunhua. Pathogenicity test showed that only 3 strains of F. solani had no pathogenicity to Hefeng 55, but the other 101 strains had different degrees of pathogenicity to Hefeng 55.
参考文献/References:
［1］Roy K W,Rupe J C, Hershman D E, et al. Sudden death syndrome of soybean［J］. Plant Disease, 1997, 81(10): 1100-1111.［2］Binod B S, Jordan L B, Prashant S, et al. Investigation of the Fusarium virguliforme transcriptomes induced during infection of soybean roots suggests that enzymes with hydrolytic activities could play a major role in root necrosis［J］. PLoS One, 2017, 12(1): e0169963.［3］Hartman G L, Chang H X, Leandro L F. Research advances and management of soybean sudden death syndrome［J］.Crop Protection, 2015, 73: 60-66.［4］Hartman G L, Huang Y H, Nelson R L, et al. Germplasm evaluation of Glycine max for resistance to Fusarium solani, the causal organism of sudden death syndrome［J］. Plant Disease, 1999, 81(5): 515-518.［5］Zhou Q, Chen Y, Yang Y, et al. Effect of inoculum density and quantitative PCR-based detection of Rhizoctonia solani AG-2-1 and Fusarium avenaceum on canola［J］. Crop Protection, 2014, 59: 71-77.［6］Zhang J X,Xue A G, Cober E R, et al. Prevalence, pathogenicity and cultivar resistance of Fusarium and Rhizoctonia species causing soybean root rot［J］. Canadian Journal of Plant Science, 2013, 93(2): 221-236.［7］Zhang J X,Xue A G, Zhang H J, et al. Response of soybean cultivars to root rot caused by Fusarium species［J］. Canadian Journal of Plant Science, 2010, 90(5): 767-776.［8］Xue A G, Cober E, Voldeng H D, et al. Evaluation of the pathogenicity of Fusarium graminearum and Fusarium pseudograminearum on soybean seedlings under controlled conditions［J］. Canadian Journal of Plant Pathology, 2007, 29(1): 35-40.［9］Diaz A,Munkvold G P, Leandro L F. First report of Fusarium proliferatum causing root rot on soybean(Glycine max) in the United States［J］. Plant Disease, 2011, 95(10): 1316-1316.［10］Diaz A, Leandro L F, Munkvold G P. Aggressiveness of Fusarium species and impact of root infection on growth and yield of soybean［J］. Phytopathology, 2013, 103(8): 822-832.［11］Ellis M L, Díaz Arias M M, Leandro L F, et al. First report of Fusarium armeniacum causing seed rot and root rot on soybean(Glycine max) in the United States［J］. Plant Disease, 2012, 96(11): 1693-1693.［12］Chang K F, Hwang S F, Conner R L, et al. First report of Fusarium proliferatum causing root rot in soybean(Glycine max L.) in Canada［J］. Crop Protection, 2015, 67: 52-58.［13］Costa S S, Matos K S, Tessmann D J, et al. Fusarium paranaense sp. nov. a member of the Fusarium solani species complex causes root rot on soybean in Brazil［J］. Fungal Biology, 2016, 120(1): 51-60.［14］Leslie J F. Fusarium spp. from corn, sorghum, and soybean fields in the central and eastern United States［J］. Phytopathology, 1990, 80(4): 343-350.［15］Kuhnem P R, Del Ponte E M, Dong Y, et al. Fusarium graminearum isolates from wheat and maize in New York show similar range of aggressiveness and toxigenicity in cross-species pathogenicity tests［J］. Phytopathology, 2015, 105(4): 441-448.［16］Harris L J,Balcerzak M, Johnston A, et al. Host-preferential Fusarium graminearum gene expression during infection of wheat, barley, and maize［J］. Fungal Biology, 2016, 120(1): 111-123.［17］韩庆新, 辛惠普. 大豆根腐病主要病原菌对大豆幼苗致病性的初步研究［J］. 大豆科学, 1990, 9(2): 157-162. (Han Q X, Xin H P. A preliminary study on the pathogenicity of the main pathogens causing soybean seedling root rot［J］. Soybean Science, 1990, 9(2): 157-162.)［18］李长松, 罗瑞梧, 杨崇良,等. 黄淮地区大豆根腐病菌分离鉴定及其致病性研究［J］. 植物保护学报, 1996, 23(2): 187-188. (Li C S, Luo R W, Yang C L, et al. Isolation and identification of the pathogenic fungi causing soybean root rot in Yellow River and Huaihe River basins［J］. Journal of Plant Protection, 1996, 23(2): 187-188.)［19］杨帅. 大豆镰刀菌根腐病病原种类及大豆抗病种质资源筛选［D］. 南京: 南京农业大学, 2012. (Yang S. Soybean Fusarium root rot pathogen species and soybean germplasm screening［D］. Nanjing: Nanjing Agricultural University, 2012.)［20］Chang X L, Dai H, Wang D P, et al. Identification of Fusarium species associated with soybean root rot in Sichuan province, China ［J］. European Journal of Plant Pathology, 2018, 151(5): 1-15.［21］Leslie J F,Summerell B A. The Fusarium laboratory manual［M］. UK: Blackwell Publish Professional, 2006.［22］Leslie J F, Anderson L L, Bowden R L, et al. Inter- and intra- specific genetic variation in Fusarium［J］. International Journal of Food Microbiology, 2007, 119(1-2): 25-32.［23］O’Donnell K, Ward T J, Robert V A R, et al. DNA sequence-based identification of Fusarium: Current status and future directions［J］. Phytoparasitica, 2015, 43(5): 583-595.［24］陈锋菊, 李百元, 杨冰, 等. 一种经济快速提取丝状真菌基因组DNA的方法［J］. 生命科学研究, 2010, 14(2): 122-149. (Chen F J, Li B Y, Yang B, et al. An economical and rapid extraction method for genomic DNA from filamentous fungi［J］. Life Science Research, 2010, 14(2): 122-149.)［25］White T J, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics ［J］. PCR Protocols, 1990, 38: 315-322.［26］Matheny P. Improving phylogenetic inference of mushrooms with RPB1 and RPB2 nucleotide sequences(Inocybe; Agaricales)［J］. Molecular Phylogenetics and Evolution, 2005, 35(1): 1-20.［27］Zhang D,Gao F L, Jakovli I, et al. PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies［J］. Molecular Ecology Resources, 2020, 20(1): 348-355.［28］喻锦秀, 吴品珊, 高必达, 等. 大豆猝死综合症病原菌北美种的PCR鉴定［J］. 植物病理学报, 2007, 37(1): 36-41. (Yu J X, Wu P S, Gao B D, et al. Use of allele-specific PCR of translation elongation factor 1-α gene to identify soybean sudden death syndrome pathogen(Fusarium virguliforme)［J］. Acta Phytopathologica Sinica, 2007, 37(1): 36-41.)［29］洪坚平, 贺冰, 郭明霞. 山西镰刀菌［M］. 北京: 中国林业出版社, 2015. (Hong J P, He B, Guo M X. Shanxi Fusarium［M］. Beijing: China Forestry Publishing House, 2015.)［30］姚艳. 黄淮地区大豆品种对根部主要病原真菌的抗性研究［D］. 南京: 南京农业大学, 2016: 34-36. (Yang Y. Resistance research of soybean varieties to the major of roots fungal pathogens in the Huang-Huai region［D］. Nanjing: Nanjing Agricultural University, 2016: 34-36.)［31］王晓艳,文景芝. 东北三省大豆根腐镰孢菌种类及其致病力分析［J］.中国油料作物学报, 2011, 33(4): 391-395. (Wang X Y, Wen J Z. Species and pathogenicity of Fusarium causing soybean root rot in northeast China［J］. Chinese Journal of Oil Crop Sciences, 2011, 33(4): 391-395.)［32］Mandeel Q A. Biodiversity of the genus Fusarium in saline soil habitats［J］. Journal of Basic Microbiology, 2010, 46(6): 480-494.［33］曾莉莎, 吕顺, 刘文清, 等. 基于多基因序列分析对尖孢镰孢菌古巴专化型(香蕉枯萎病菌)生理小种的鉴定［J］.菌物学报, 2014, 33(4): 867-882. (Zeng L S, Lyu S, Liu W Q, et al. The identification on race of Fusarium oxysporum f. sp.cubense based on multi-gene sequence analysis［J］. Mycosystema, 2014, 33(4): 867-882.)
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(03):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(03):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(03):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(03):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(03):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]李春杰,刘海龙,许艳丽.大豆根腐病拮抗细菌的生物学特性研究[J].大豆科学,2011,30(05):809.[doi:10.11861/j.issn.1000-9841.2011.05.0809]
LI Chun-jie,LIU Hai-long,XU Yan-li.Biological Characteristics of Antagonistic Bacteria against Soybean Root Rot[J].Soybean Science,2011,30(03):809.[doi:10.11861/j.issn.1000-9841.2011.05.0809]
[12]刘金波,许艳丽,李春杰,等.大豆连作土壤盆栽大豆根腐病及生长发育状况[J].大豆科学,2008,27(05):806.[doi:10.11861/j.issn.1000-9841.2008.05.0806]
LIU Jin-bo,XU Yan-li,LI Chun-jie,et al.Effect of Long Term Soybean Monoculture on Soybean Root Rot and Soybean Growth and Development in Pot Experiment[J].Soybean Science,2008,27(03):806.[doi:10.11861/j.issn.1000-9841.2008.05.0806]
[13]许艳丽,张红骥,张匀华,等.施用根腐病生防颗粒剂对大豆田土壤微生物区系的影响[J].大豆科学,2007,26(02):198.[doi:10.3969/j.issn.1000-9841.2007.02.017]
XU Yan-liZHANG Hong-ji,ZHANG Yun-hua,LI Chun-jie.THE EFFECT OF BIOCONTROL AGENTS OF TRICHODERMA AGAINST SOYBEAN ROOT ROT ON SOIL MICROORGANISM[J].Soybean Science,2007,26(03):198.[doi:10.3969/j.issn.1000-9841.2007.02.017]
[14]台莲梅郭永霞林纯刚闫凤云辛惠普.不同农业措施对重茬大豆根腐病及大豆生育的影响[J].大豆科学,2002,21(04):298.[doi:10.11861/j.issn.1000-9841.2002.04.0298]
Tai Lianmei Guo Yongxia Lin Cungang Yan Fengyun Xin Huipu.EFFECT ON AGRONOMIC PRACTICES TO SOYBEAN ROOT ROT AND SOYBEANGROWTH IN CONDITION OF CONTINUOUS CROPPING[J].Soybean Science,2002,21(03):298.[doi:10.11861/j.issn.1000-9841.2002.04.0298]
[15]韩丽梅 鞠会艳 王旭明.大豆连作土壤有机化合物对大豆根腐病菌生长的影响[J].大豆科学,2004,23(01):36.[doi:10.11861/j.issn.1000-9841.2004.01.0036]
Han Limei Qu Huyan Wang Xuming.INFLUENCE OF THEORGANIC COMPOUNDS IN CONTINUOUS CROPPINGSOYBEAN ON PATHOGENIC OF ROOT ROT[J].Soybean Science,2004,23(03):36.[doi:10.11861/j.issn.1000-9841.2004.01.0036]
[16]于晓波,梁建秋,何泽民,等.不同根腐病防控方法对四川省间作大豆农艺性状和产量影响[J].大豆科学,2021,40(05):669.[doi:10.11861/j.issn.1000-9841.2021.05.0669]
YU Xiao-bo,LIANG Jian-qiu,HE Ze-min,et al.Effects of Different Root Rot Control Methods on Agronomic Characters and Yield of Intercropping Soybean in Sichuan[J].Soybean Science,2021,40(03):669.[doi:10.11861/j.issn.1000-9841.2021.05.0669]
备注/Memo