LI Zhao-jun,TIAN Ru-mei,GONG Yong-chao,et al.Identification of Salt Tolerance of Soybean in Germination Stage Based on Phenotype and Antioxidant Enzyme Activity[J].Soybean Science,2020,39(01):76-83.[doi:10.11861/j.issn.1000-9841.2020.01.0076]
基于表型和抗氧化酶活性的大豆萌芽期耐盐性鉴定
- Title:
- Identification of Salt Tolerance of Soybean in Germination Stage Based on Phenotype and Antioxidant Enzyme Activity
- Keywords:
- Soybean; Salt tolerance identification; Phenotype; Antioxidant enzyme activity; Correlation analysis
- 文献标志码:
- A
- 摘要:
- 为促进在大豆种植初期以表型和抗氧化酶活性评价和筛选耐盐大豆资源，缓解山东省土壤盐渍化对大豆生产的影响，以山东省9份主推大豆品种为材料，在其萌芽期进行4个梯度NaCl胁迫处理，测定其表型指标、抗氧化酶活性和耐盐性，并分析各指标与品种耐盐性的相关性，探究综合评价大豆品种耐盐性的表型和抗氧化酶指标。结果表明：NaCl胁迫浓度高于1%可显著降低大豆发芽率、胚根长和须根数；随着盐浓度不断升高，抗氧化酶活性呈下降趋势，且与对照抗氧化酶活性差异显著；品种耐盐性分级与盐胁迫浓度相关，耐盐品种菏豆28、齐黄34在不同NaCl胁迫条件下始终维持较高水平的抗氧化酶活性；SOD活性是大豆萌芽期耐盐性鉴定评价的重要指标，胚根长、须根数及CAT、POD活性是高浓度NaCl胁迫（1% NaCl）下，大豆耐盐性评价的关键指标。本研究为大豆耐盐性的快捷鉴定评价提供选择指标和数据支撑，能够促进耐盐大豆种质创新及优良亲本筛选。
- Abstract:
- In order to evaluation and screening soybean resources of salt-tolerant at early planting stage to alleviate the impact of soil salinization on soybean production in Shandong province, this study selected 9 main soybean varieties in Shandong province as materials, and carried out four gradient NaCl stress treatments in the germination stage. We measured the phenotypic indexes, antioxidant enzyme activities and salt tolerance of soybean at different NaCl stress, and then investigated the correlation of these indexes with salt tolerance of the varieties. The results showed that the germination rate, radicle length and number of fibrous roots of soybean were significantly reduced when the treatment of NaCl stress concentration was higher than 1%. As the salt concentration increased, the activity of antioxidant enzymes showed a downward trend and was significantly different from the antioxidant enzyme activity of the control group. Salt tolerance grading of varieties was relative with salt stress concentration, the antioxidant enzyme activity of salt-tolerant varieties Hedou 28 and Qihuang 34 maintained high level under different NaCl stress conditions. SOD activity was an important indicator for the evaluation of soybean salt tolerance at germination stage. The radicle length, number of fibrous roots and CAT, POD activity were the key indicators for the evaluation of salt tolerance at high concentration NaCl stress (1% NaCl). This study provides selecting index and data support for the evaluation of salt tolerance of soybean in the most economical and effective way, and can promote the screening of excellent parent material for the innovation of salt-tolerant soybean germplasm.
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备注/Memo
收稿日期：2019-07-02