[1]曹亮,王明瑶,邹京南,等.外源褪黑素对干旱胁迫下大豆鼓粒期生长特性的影响[J].大豆科学,2019,38(05):747-753.[doi:10.11861/j.issn.1000-9841.2019.05.0747]
　CAO Liang,WANG Ming-yao,ZOU Jing-nan,et al.Effects of Exogenous Melatonin on the Growth Characteristics of Soybean During Seed-filling Period Under Drought Stress[J].Soybean Science,2019,38(05):747-753.[doi:10.11861/j.issn.1000-9841.2019.05.0747]
点击复制

外源褪黑素对干旱胁迫下大豆鼓粒期生长特性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年05期 页码: 747-753 栏目: 出版日期: 2019-09-20
Title:
Effects of Exogenous Melatonin on the Growth Characteristics of Soybean During Seed-filling Period Under Drought Stress
作者:
（黑龙江八一农垦大学 农学院，黑龙江 大庆 163319）
Author(s):
(Agronomy College of Heilongjiang Bayi Agricultural University, Daqing 163319, China)
关键词:
大豆; 鼓粒期; 干旱胁迫; 褪黑素; 光合产物; 产量
Keywords:
Soybean (Glycine max L.);  Seed-filling period;  Drought stress;  Melatonin;  Photosynthetic products;  Yield
DOI:
10.11861/j.issn.1000-9841.2019.05.0747
文献标志码:
A
摘要:
鼓粒期是决定豆科作物产量高低的关键生育期，为研究大豆鼓粒期干旱胁迫及褪黑素的调控效应，于大豆鼓粒初期进行干旱胁迫和褪黑素处理，比较不同处理间的形态指标、产量及其构成因素、光合参数和碳代谢指标。结果表明：鼓粒期干旱胁迫导致单株荚数、单株粒数、百粒重和单株粒重显著减少，较对照分别减少了9.04%、6.21%、22.81%和27.44%。随着干旱胁迫时间的延长，叶片Pn、Tr和Gs逐渐降低，Ci逐渐升高，叶片内的糖类物质含量（淀粉、蔗糖、果糖和葡萄糖）逐渐降低，转化酶活性（酸性和中性）、蔗糖磷酸合成酶和蔗糖合成酶活性受到了显著的抑制。而施用褪黑素无论是在正常环境还是干旱胁迫下均可以改善叶片的光合参数，不同程度地增加大豆的单株荚数和单株粒数，最终显著提高了大豆产量，褪黑素处理使正常环境下的大豆产量提高4.74%，使干旱环境下的大豆产量提高12.43%。但正常环境下施用褪黑素对大豆叶片糖类物质含量及其相关酶活性无显著影响，干旱胁迫下施用褪黑素却显著提高了大豆叶片内的糖类物质含量及其相关酶活性，表明褪黑素可以有效地缓解干旱胁迫对植物生长发育造成的不利影响。
Abstract:
Seed-filling stage is the key growth period that determines the level of soybean yield. This experiment was conducted at the beginning of seed-filling in order to study the effects of drought stress and melatonin through comparing morphological indexes, yield and its components, photosynthetic parameters and carbon metabolic indicators. The results showed that drought stress caused a significant reduction in pod number per plant (9.04%), particle number per plant (6.21%), 100-seed weight (22.81%) and particle weight per plant (27.44%). With prolonged drought treatment, net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) were significantly reduced, and intercellular CO2 concentration (Ci) was markedly increased. The levels of starch, sucrose, fructose and glucose were declined, and acid invertase, neutral invertase, sucrose phosphate synthase and sucrose synthase activities were restrained during drought stress treatment. Under normal and drought environments, melatonin pre-treatment caused a increase in pod number per plant, particle number per plant and yield because of photosynthetic parameters were improved. Melatonin pre-treatment caused a increase in yield under normal and drought (4.74% and 12.43%) environments. But melatonin treatment had no significant effect on carbohydrate contents and related enzyme activities. Under drought stress, melatonin pre-treatment increased carbohydrate contents and related enzyme activities. It indicates that melatonin can effectively alleviate the adverse effects of drought stress on plant growth and development.

参考文献/References:

［1］Harrison M T, Tardieu F, Dong Z, et al. Characterizing drought stress and trait influence on maize yield under current and future conditions［J］. Global Change Biology, 2014, 20(3):867-878.
［2］Lobell D B, Roberts M J, Schlenker W, et al. Greater sensitivity to drought accompanies maize yield increase in the U.S. Midwest.［J］. Science, 2014, 344(6183): 516-519.
［3］Mayank, Anand, Gururani, et al. Regulation of photosynthesis during abiotic stress-induced photoinhibition［J］. Molecular Plant, 2015, 8(9): 1304-1320.
［4］Chaves M M, Flexas J, Pinheiro C. Photosynthesis under drought and salt stress: Regulation mechanisms from whole plant to cell［J］. Annals Botany, 2009, 103(4): 551-560.
［5］曹秀清, 蒋尚明. 干旱胁迫对大豆品质及产量的影响［J］. 现代农业科技, 2017(16): 3-4, 7. (Cao X Q, Jiang S M. Effect of drought stress on yield and quality of soybean［J］. Modern Agricultural Technology, 2017(16): 3-4, 7.)
［6］张仟雨, 李萍, 宗毓铮, 等. 干旱对大豆生理及产量影响的研究［J］. 华北农学报, 2016, 31(5): 140-145. (Zhang Q Y, Li Q, Zong Y Z, et al. Effects of drought on physiology and yield of soybean［J］. Acta Agriculturae Boreali-Sinica, 2016, 31(5): 140-145.)
［7］Graham I A. Carbohydrate control of gene expression in higher plants［J］. Research in Microbiology, 1996, 147(6-7): 572-580.
［8］Maria R, Zvi P, Yasser M, et al. Stress-induced cytokinin synthesis increases drought tolerance through the coordinated regulation of carbon and nitrogen assimilation in rice［J］. Plant Physiology, 2013, 163(4): 1609-1622.
［9］刘文夫, 董守坤, 徐亚会, 等. 大豆苗期干旱胁迫对糖分吸收与相关酶活性的影响［J］. 作物杂志, 2014(3): 117-120. (Liu W F, Dong S K, Yu Y H, et al. Effects of drought stress on sugar absorption and related enzyme activities at soybean seedling［J］. Crops, 2014(3): 117-120.)
［10］Zhang N, Zhang H J, Zhao B, et al. The RNA-seq approach to discriminate gene expression profiles in response to melatonin on cucumber lateral root formation［J］. Journal of Pineal Research, 2013, 56(1): 39-50.
［11］邹京南, 金喜军, 王孟雪, 等. 外源褪黑素对干旱胁迫条件下大豆苗期光合及生理的影响［J］. 大豆科学, 2018, 37(6): 896-905. (Zou J N, Jin X J, Wang M X, et al. Effects of exogenous melatonin on photosynthesis and physiology of soybean seedlings under drought stress［J］. Soybean Science, 2018, 37(6): 896-905.)
［12］Ming D F, Pei Z F, Naeem M S, et al. Silicon alleviates PEG-induced water-deficit stress in upland rice seedlings by enhancing osmotic adjustment［J］. Journal of Agronomy & Crop Science, 2012, 198(1):14-26.
［13］Je′ro^me Verdier, Richard D T. Transcriptional regulation of storage protein synthesis during dicotyledon seed filling［J］. Plant Cell Physiol, 2008, 49(9): 1263-1271.
［14］Courtney D N, Jocelyn A O, Leonid V K, et al. Tissue-specific regulation of gibberellin biosynthesis in developing pea seeds［J］. Plant Physiology, 2011, 156(2):897-912.
［15］张宪政. 作物生理研究法［M］. 北京: 中国农业出版社, 1992. (Zhang X Z. Crop physiology research［M］. Beijing: China Agricultural Press, 1992.)
［16］张志良. 植物生理学实验指导［M］. 北京: 高等教育出版社, 2001: 128-129. (Zhang Z L. Plant physiology experiment guide［M］. Beijing: Higher education press, 2001: 128-129.)
［17］Tsai C Y, Salamini F, Nelson O E. Enzymes of carbohydrate metabolism in the developing endosperm of maize［J］. Plant Physiology, 1970, 46(2): 299-306.
［18］Chopra J, Kaur N, Gupta A K. Ontogenic changes in enzymes of carbon metabolism in relation to carbohydrate status in developing mungbean reproductive structures［J］. Phytochemistry, 2000, 53(5):539-548.
［19］屈春媛, 张玉先, 金喜军, 等. 干旱胁迫下外源ABA对鼓粒期大豆产量及氮代谢关键酶活性的影响［J］. 中国农学通报, 2017, 33(34): 26-31. (Qu C Y, Zhang Y X, Jin X J, et al. Effect of exogenous aba on yield and key enzyme activities of nitrogen metabolism of soybean under drought stress［J］. Chinese Agricultural Science Bulletin, 2017, 33(34): 26-31.)
［20］李菁华. 干旱胁迫下外源ABA对鼓粒期大豆抗旱生理特性及产量的影响［D］. 大庆: 黑龙江八一农垦大学, 2017. (Li J H. Effects of exogenous aba on physiological characteristics and yield of soybean in drought stress under drought stress ［D］. Daqing: Heilongjiang Bayi Agricultural University, 2017.)
［21］Jia H, Oguchi R, Hope A, et al. Differential effects of severe water stress on linear and cyclic electron fluxes through Photosystem I in spinach leaf discs in CO2-enriched air［J］. Planta, 2008, 228: 803-812.
［22］Hu W H, Yan X H, Xiao Y A, et al. 24-Epibrassinosteroid alleviate drought-induced inhibition of photosynthesis in Capsicum annuum［J］. Scientia Horticulturae, 2013, 150(2): 232-237.
［23］Yin C, Peng Y, Zang R, et al. Adaptive responses of populus kangdingensis to drought stress［J］. Physiologia Plantarum, 2010, 123(4): 445-451.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(05):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(05):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(05):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(05):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(05):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(05):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(05):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(05):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(05):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(05):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]李得孝,刘修杰,胡超,等.大豆节位和粒位对荚果厚度的影响[J].大豆科学,2014,33(06):841.[doi:10.11861/j.issn.1000-9841.2014.06.0841]
　LI De-xiao,LIU Xiu-jie,HU Chao,et al.Effect of Node and Seed Position on Pod Thickness Related Indexes in Soybean[J].Soybean Science,2014,33(05):841.[doi:10.11861/j.issn.1000-9841.2014.06.0841]
[12]李菁华,张明聪,金喜军,等.高油型和高蛋白型大豆鼓粒期的糖分积累规律[J].大豆科学,2017,36(01):68.[doi:10.11861/j.issn.1000-9841.2017.01.0068]
　LI Jing-hua,ZHANG Ming-cong,JIN Xi-jun,et al.Sugar Accumulation Rule of High Oil and High Protein Soybean During the Seed-Filling Period[J].Soybean Science,2017,36(05):68.[doi:10.11861/j.issn.1000-9841.2017.01.0068]
[13]李得孝,梁福琴,汪勇,等.利用荚果厚度与粒重估计大豆鼓粒速率的研究[J].大豆科学,2017,36(02):244.[doi:10.11861/j.issn.1000-9841.2017.02.0244]
　LI Dexiao,LIANG Fuqin,WANG Yong,et al.Estimates of Seed Filling Rate Based on Dynamics of Pod Thickness and Seed Weight in Soybean[J].Soybean Science,2017,36(05):244.[doi:10.11861/j.issn.1000-9841.2017.02.0244]
[14]唐子竣,向友珍,王辛,等.基于不同分析模型的大豆叶片SPAD值和LAI光谱估算比较[J].大豆科学,2023,42(01):55.[doi:10.11861/j.issn.1000-9841.2023.01.0055]
[15]黄深富,郑殿峰,项洪涛.花期和鼓粒期低温胁迫对大豆叶片生理特性及产量的影响[J].大豆科学,2024,43(01):64.[doi:10.11861/j.issn.10009841.2024.01.0064]

备注/Memo

收稿日期：2019-03-18

基金项目：国家重点研发计划（2018YFD0201000）；国家现代农业产业技术体系（CARS-04-01A）。
第一作者简介：曹亮（1990-），男，博士，主要从事大豆逆境栽培生理方面的研究。E-mail：miss9877@126.com。
通讯作者：张玉先（1968-），男，博士，教授，博导，主要从事大豆栽培生理研究。E-mail：zyx_lxy@126.com。

更新日期/Last Update: 2019-09-20