[1]刘风刚,巨鹏飞,王福政,等.三唑酮对大豆花期干旱胁迫下根系AsA-GSH循环的影响[J].大豆科学,2019,38(05):740-746.[doi:10.11861/j.issn.1000-9841.2019.05.0740]
　LIU Feng-gang,JU Peng-fei,WANG Fu-zheng,et al.Effect of Triadimefon on AsA-GSH Cycle of Soybean Roots Under Drought Stress at Flowering Stage[J].Soybean Science,2019,38(05):740-746.[doi:10.11861/j.issn.1000-9841.2019.05.0740]
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三唑酮对大豆花期干旱胁迫下根系AsA-GSH循环的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年05期 页码: 740-746 栏目: 出版日期: 2019-09-20
Title:
Effect of Triadimefon on AsA-GSH Cycle of Soybean Roots Under Drought Stress at Flowering Stage
作者:
刘风刚; 巨鹏飞; 王福政; 张守忠; 邢邯; 江海东
（南京农业大学 农业部作物生理生态与生产管理重点实验室/江苏省现代作物生产协同创新中心/国家大豆改良中心，江苏 南京 210095）
Author(s):
（Key Laboratory of Crop Physiology Ecology and Production Management,Ministry of Agriculture, Jiangsu Collaborative Innovation Center for Modern Crop Production, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing 210095, China）
关键词:
三唑酮; 大豆; 干旱胁迫; 根系; AsA-GSH循环
Keywords:
Triadimefon;  Soybean;  Drought stress;  Roots;  Ascorbate-Glutathione Cycle
DOI:
10.11861/j.issn.1000-9841.2019.05.0740
文献标志码:
A
摘要:
为研究干旱胁迫下喷施三唑酮对大豆花期根系耐旱性的影响，以大豆品种南农99-6为材料，通过盆栽试验，在初花期喷施三唑酮后，开始干旱胁迫处理，土壤相对含水量逐渐降至45%，干旱持续21 d后复水，研究干旱胁迫下喷施三唑酮对大豆根系膜脂过氧化程度、活性氧和抗坏血酸-谷胱甘肽（AsA-GSH）循环系统的影响。结果表明：相较于第21天的干旱（D）处理，干旱喷施三唑酮（TD）处理使整个花期干旱胁迫下根系中MDA含量降低了18.75%；O-2〖KG-3mm〗?产生速率和H2O2含量分别减少了10.05%和8.55%。TD处理增加了整个花期干旱胁迫下APX和GR活性；干旱胁迫后期增加了MDHAR活性并延缓了GPX和DHAR活性的降低。TD处理的第21天的AsA和GSH含量分别高于D处理17.67%和18.12%。在干旱复水后7 d TD比D处理维持了较高的抗氧化酶活性和抗氧化剂含量。以上结果表明：喷施三唑酮提高了干旱胁迫下大豆根系中抗氧化酶的活性，增强了AsA和GSH的再生能力，增加了抗氧化物质的含量，促进了大豆根系中活性氧的清除，减轻了膜脂过氧化程度。而复水后较高的抗氧化酶活性和抗氧化剂，促进了根系向正常生理状态的恢复。因此TD处理增强了大豆的抗旱性。
Abstract:
In order to study the effects of spraying triadimefon on the drought tolerance of soybean roots under drought stress at flowering stage, the soybean variety Nannong 99-6 was used as the test material. After spraying triadimefon at the initial flowering stage, the drought stress treatment was started. The soil relative water content gradually decreased to 45%, and the drought continued for 21 d and then rehydrated. The effects of spraying triadimefon on the membrane lipid peroxidation, reactive oxygen species and ascorbate-glutathione cycle in soybean roots under drought stress were studied. The results showed that TD reduced the MDA content in the roots by 18.75%, the O-2? production rate and H2O2 content decreased by 10.05% and 8.55% respectively, compared with the D treatment on the 21st day. TD treatment increased the activity of APX and GR under drought stress during the whole flowering period, increased the activity of MDHAR and delayed the decrease of GPX and DHAR activity at the late stage of drought stress. The AsA and GSH contents on the 21 d of TD treatment were 17.76% and 18.12% higher than those of D treatment, respectively. Compared with D treatment, TD treatment maintained higher antioxidant enzyme activity and antioxidant content in 7 d after drought rehydration. The above results indicated that spraying triadimefon increased the activity of antioxidant enzymes in soybean roots under drought stress, enhanced the regeneration ability of AsA and GSH, increased the content of antioxidants, promoted the removal of reactive oxygen species in soybean roots, and alleviated the membrane lipid peroxidation. The higher antioxidant enzyme activity and antioxidant content after rehydration promoted the recovery of roots to normal physiological state. Therefore, TD treatment enhanced the drought resistance of soybean.

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备注/Memo


收稿日期：2019-03-05
基金项目：国家重大研发项目(2017YFD0101500)。第一作者简介：刘风刚（1991-），男，硕士，主要从事作物生理生态研究。E-mail：2016101021@njau.edu.cn。通讯作者：江海东（1968-），男，博士，副教授，主要从事油料作物栽培和逆境生理研究。E-mail：hdjiang@njau.edu.cn。

更新日期/Last Update: 2019-09-20