[1]王梦奇,白庆荣,王大川,等.吉林省大豆茎点霉叶斑病病原鉴定[J].大豆科学,2019,38(03):428-433.[doi:10.11861/j.issn.1000-9841.2019.03.0428]
　WANG Meng-qi,BAI Qing-rong,WANG Da-chuan,et al.Pathogen Identification of Soybean Leaf Spot Caused by Boeremia exigua var. exigua in Jilin Province[J].Soybean Science,2019,38(03):428-433.[doi:10.11861/j.issn.1000-9841.2019.03.0428]
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吉林省大豆茎点霉叶斑病病原鉴定

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年03期 页码: 428-433 栏目: 出版日期: 2019-05-20
Title:
Pathogen Identification of Soybean Leaf Spot Caused by Boeremia exigua var. exigua in Jilin Province
作者:
（1.吉林农业大学 农学院，吉林 长春 130118； 2.吉林省农业技术推广总站，吉林 长春 130033）
Author(s):
WANG Meng-qi1; BAI Qing-rong1; WANG Da-chuan2; YANG Li-na1
（1.College of Agronomy，Jilin Agricultural University，Changchun 130118，China; 2.Jilin Province Agricultural Technology Extension Station，Changchun 130033，China）
关键词:
大豆; Boeremia exigua var.exigua; 鉴定
Keywords:
Soybean; Boeremia exigua var.exigua; Identification
DOI:
10.11861/j.issn.1000-9841.2019.03.0428
文献标志码:
A
摘要:
从吉林省白山市靖宇县的大豆叶片上分离到一种真菌分离物，根据菌落形态特征、分生孢子形态特征、ITS、ACT、tub2、rpb2、TEF、LSU序列、致病性测试结果以及上述6种引物的系统发育树构建分析，将其鉴定为Boeremia exigua var.exigua，这是在我国首次发现该真菌感染大豆。系统发育树分析结果中，rpb2和TEF基因在种间关系上显示出了较好的区分度，更适用于Boeremia属的鉴定及研究种间关系。
Abstract:
The strain was identified as Boeremia exigua var.exigua, which was collected form Jingyu County of Baishan City in Jilin, based on the morphological characteristics of the colony and conidia，rDNA-ITS,partial actin gene,beta-tubulin gene,RNA polymerase II second largest subunit gene,translation elongation factor 1 alpha gene,large subunit ribosomal RNA gene, the pathogenicity to young soybean, phylogenetic tree construction and analysis of the above 6 primers. It was the first report that Boeremia exigua var.exigua infected soybean in China.In the phylogenetic tree analysis results, rpb2 and TEF gene show a good discrimination in interspecies relationship, they are more suitable for identifying and studying intergenetic relationships of Boeremia.

参考文献/References:

［1］刘志勇.大豆种子主要寄藏真菌及其分布［D］.哈尔滨：东北农业大学, 2012. (Liu Z Y.The main genraand distribution of seed-borne［D］.Harbin:Northeast Agricultural University, 2012.)
［2］马淑梅, 丁俊杰,顾鑫,等.黑龙江省大豆主要病害发生危害调査［J］.黑龙江农业科学, 2005(6):48-52. (Ma S M, Ding J J, Gu X, et al.Survey on the occurrence and damage in main disease of soybean in Heilongjiang province［J］.Heilongjiang Agricultural Science, 2005(6):48-52.)
［3］李沐慧, 王媛媛, 陈井生, 等. 2015年东北地区大豆田病害种类与危害程度调查研究［J］. 大豆科学, 2016, 35(4):643-648.(Li M H, Wang Y Y, Chen J S, et al. Incidence and disease index of soybean diseases in the Northeast of China in 2015［J］.Soybean Science, 2016, 35(4):643-648.)
［4］李绍兰, 周斌, 杨丽源, 等. 真菌DNA提取方法的改良［J］. 云南大学学报：自然科学版，2002, 24 (6): 471-472.(Li S L, Zhou B, Yang L Y, et al. An improved method for extracting fungal DNA［J］. Journal of Yunnan University: Natural Sciences Edition, 2002, 24 (6): 471-472.)
［5］White T J, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics［M］// Innis M A,Gelfand D H, Sninsky J J, et al. Pcr Protocols:A guide to methods and applications. California:Academic Press, 1990:315-322.
［6］Carbone I, Kohn L M. A method for designing primer sets for speciation studies in filamentous ascomycetes［J］. Mycologia, 1999: 553-556.
［7］Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species［J］. Journal of Bacteriology, 1990, 172(8):4238-4246.
［8］Rehner S A, Samuels G J.Taxonomy and phylogeny of Gliocladium, analysed from nuclear large subunit ribosomal DNA sequences［J］. Mycological Research, 1994, 98(6):625-634.
［9］Groenewald J Z, Nakashima C, Nishikawa J, et al. Species concepts in Cercospora: Spotting the weeds among the roses［J］. Studies in Mycology, 2013, 75(1):115-170.
［10］Liu Y J, Whelen S, Hall B D. Phylogenetic relationships among ascomycetes: Evidence from an RNA polymerse II subunit［J］. Molecular Biology & Evolution, 1999, 16(12):1799.
［11］Carbone I, Kohn L M. A method for designing primer sets for speciation studies in Filamentous Ascomycetes［J］. Mycologia, 1999, 91(3):553-556.
［12］Aveskamp M M, de Gruyter J, Woudenberg J H, et al. Highlights of the Didymellaceae: A polyphasic approach to characterise Phoma and related pleosporalean genera［J］. Studies in Mycology, 2010, 65(65):1-60.
［13］Schoch C L, Crous P W, Groenewald J Z, et al. A class-wide phylogenetic assessment of Dothideomycetes［J］. Studies in Mycology, 2009, 64(64):1-15.
［14］Michel V V, Daepp M, Woudenberg J H C,et al. First report of Boeremia exigua var.exigua causing stem and leaf spot on common speedwell in Switzerland ［J］. Plant Disease, 2018, 102(2):440.
［15］Aveskamp M M, Verkley G J M, Gruyter J D, et al. DNA phylogeny reveals polyphyly of Phoma Section Peyronellaea and multiple Taxonomic Novelties［J］. Mycologia, 2009, 101(3):363-382.
［16］de G J, Aveskamp M M, Woudenberg J H, et al. Molecular phylogeny of Phoma and allied anamorph genera: Towards a reclassification of the Phoma complex［J］. Mycological Research, 2009, 113(4):508-519.
［17］Vu D, Groenewald M, Vries M D, et al. Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom Fungi and reveals thresholds for fungal species and higher taxon delimitation［J］. Studies in Mycology, 2019,92:135-154.
［18］Aveskamp M M, Woudenberg J H C, Gruyter J D, et al. Development of taxon-specific sequence characterized amplified region (SCAR) markers based on actin sequences and DNA amplification fingerprinting (DAF): A case study in the Phomaexigua species complex［J］. Molecular Plant Pathology, 2010, 10(3):403-414.
［19］Kirk P, Cannon P, Minter D, et al. Dictionary of the fungi［J］. Commonwealth Mycological Institute, 2008:17-19.
［20］Boerema G H, Degruyter J, Noordeloos M E. Contributions towards a monograph of Phoma (Coelomycetes)—IV. Section Heterospora: Taxa with large sized conidial dimorphs,in vivo sometimes as Stagonosporopsis synanamorphs［J］. Persoonia - Molecular Phylogeny and Evolution of Fungi, 1997, 16 (3):335-371.
［21］Chen Q, Jiang J R, Zhang G Z, et al. Resolving the Phoma enigma［J］. Studies in Mycology, 2015,82:137-217.
［22］Marcinkowska J, Roze-Katuny I, Katuny W. Pathogenicity of some Phomaexigua var.exigua isolates［J］. Phytopathologia Polonica, 2005(38):35-44.

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备注/Memo

收稿日期：2019-01-15

基金项目：国家自然科学基金 (31500022)。
第一作者简介：王梦奇（1994-），男，硕士，主要从事植物病害综合防治研究。E-mail：392574041@qq.com。
通讯作者：白庆荣（1975-），女，教授，硕导，主要从事植物病理综合防治研究。E-mail：bbbqqqrrr@163.com。

更新日期/Last Update: 2019-05-30