[1]刘明骞,张新鑫,马佳慧,等.不同改良处理对梅州离子型稀土矿废弃地大豆根瘤菌多样性的影响[J].大豆科学,2019,38(01):77-83.[doi:10.11861/j.issn.1000-9841.2019.01.0077]
　LIU Ming-qian,ZHANG Xin-xin,MA Jia-hui,et al.The Effect of Different Restorative Treatments on the Diversity of Soybean Rhizobia in the Abandoned Land of Ionic Rare Earth Mine[J].Soybean Science,2019,38(01):77-83.[doi:10.11861/j.issn.1000-9841.2019.01.0077]
点击复制

不同改良处理对梅州离子型稀土矿废弃地大豆根瘤菌多样性的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第38卷 期数: 2019年01期 页码: 77-83 栏目: 出版日期: 2019-01-20
Title:
The Effect of Different Restorative Treatments on the Diversity of Soybean Rhizobia in the Abandoned Land of Ionic Rare Earth Mine
作者:
刘明骞1; 2; 张新鑫3; 马佳慧3; 李其斌3; 陈丽君4; 周玮2; 3; 5; 马玲2; 3; 5; 陈晓阳2; 3; 5
（1.华南农业大学 华南农业博物馆筹建办公室，广东 广州 501642； 2.广东省木本饲料工程技术研究中心，广东 广州 501642； 3.华南农业大学 林学与风景园林学院，广东 广州 501642； 4.华南农业大学 试验中心（农事训练中心），广东 广州 501642； 5.广东省森林植物种质创新与利用重点实验室，广东 广州 501642）
Author(s):
LIU Ming-qian1; 2; ZHANG Xin-xin3; MA Jia-hui3; LI Qi-bin3; CHEN Li-jun4; ZHOU Wei2; 3; 5; MA Ling2; 3; 5; CHEN Xiao-yang2; 3; 5
(1.Preparation Office of South China Agricultural Museum, South China Agricultural University, Guangzhou 510642, China; 2.Guangdong Province Research Center of Woody Forage Engineering Technology, South China Agricultural University, Guangzhou 510642, China; 3.College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China; )
关键词:
大豆; 离子型稀土矿; 根瘤菌; DGGE; 多样性
Keywords:
Soybean (Glycine max); Ionic rare earth mine; Rhizobia; DGGE; Diversity
DOI:
10.11861/j.issn.1000-9841.2019.01.0077
文献标志码:
A
摘要:
为评价梅州离子型稀土矿废弃地不同土壤改良措施对大豆根瘤菌多样性的影响，通过nifH-PCR-DGGE技术对不同土壤改良处理条件下大豆根瘤菌群落的多样性进行表征。结果表明：7种不同土壤改良处理的DGGE图谱条带数目不等，位置各异，说明梅州离子型稀土矿废弃地大豆的根瘤菌群落结构受改良措施的影响较大，具有较强的可塑造性。其中T4处理（有机肥+生物炭+钝化剂）的条带数最多，多样性指数最高，说明该处理对增加大豆根瘤菌的多样性效果最好，可作为以后土壤改良的参考依据。聚类分析结果显示：7个处理可分为4个类群，CK单独为一个类群，T1（有机肥）、T2（有机肥+生物炭）为一个类群，T4（有机肥+生物炭+钝化剂）和T5（有机肥+菌剂+蚯蚓）为一个类群，T3(有机肥+钝化剂）和T6（有机肥+菌剂+蚯蚓+生物炭+钝化剂）为一个类群。生物炭与钝化剂组合处理、菌剂与蚯蚓的组合处理对增加大豆根瘤内根瘤菌的多样性效果较显著。通过序列比对和系统发育树可以看出，在梅州黄畲地区稀土采矿迹地，大豆主要与慢生根瘤菌属菌株共生结瘤，也可与中华根瘤菌属菌株共生。大豆根瘤内根瘤菌的多样性与土壤镉含量之间为显著正相关关系，与土壤有机质、氮、磷等营养指标之间为负相关关系，说明根瘤菌可能在提高大豆耐镉方面具有重要作用。
Abstract:
In order to evaluate the effect of different improvement measures on the diversity of rhizobium of soybean (Glycine max) in the abandoned land of ionic rare earth mine, nifH-PCR-DGGE was used to manifestate the diversity of Rhizobia. The results of denaturing gradient gel electrophoresis (DGGE) fingerprint showed that there were differences in the number, the positions and the brightness of bands depended on different restorative treatments, which showed that different soil amendments had a significant effect on the community structure of Rhizobium. The number and diversity index of bands of T4 treatment (fertilizer+biochar+deactivator) were highest, it was best to improve the diversity of rhizobium of soybean. The results of cluster analysis showed that 7 treatments could be divided into four groups, CK was group 1, T1 and T2 were group 2, T4 and T5 were group 3, T3 and T6 were group 4. The combination of biochar and passivator, the combination of bacterial manure and earthworm had the more significant effect on increasing the diversity of rhizobia in root nodules of soybean. According to sequence comparison and phylogenetic tree, soybean is mainly lived in symbiosis with Bradyrhizobium, and also coexisted with Sinorhizobium in the abandoned land of ionic rare earth mine of Meizhou, Guangdong. There was significant positive correlation between the diversity of rhizobium and soil cadmium content, and negative correlation among the diversity of rhizobium and soil organic matter, nitrogen, phosphorus and other nutritional indicators, which suggesting that rhizobium may play an important role in improving the cadmium tolerance of soybean.

参考文献/References:

［1］马秀丽. 南方稀土矿治理区植物群落特征及其土壤效应［D］. 福州：福建师范大学, 2015: 1-10. (Ma X L. Plant community character and its soil effect in rare earth element mining area of Fujian province, South China［D］. Fuzhou: Fujian Normal University, 2015: 1-10.)
［2］刘文深, 刘畅, 王志威, 等. 离子型稀土矿尾砂地植被恢复障碍因子研究［J］. 土壤学报, 2015, 52(4): 879-887. (Liu W S, Liu C, Wang Z W, et al. Limiting factors for restoration of dumping sites of ionic rare earth mine tailings［J］. Acta Pedologica Sinica, 2015, 52(4): 879-887.)
［3］黄华谷, 黄铁兰, 周兆帅, 等. 广东三个离子吸附型稀土矿的地球化学特征及开采现状［J］. 岩矿测试, 2014, 33(5): 737-746. (Huang H G, Huang T L, Zhou Z S, et al. Mining situation and geochemistry characteristics of three ion adsorption rare-earth deposit in Guangdong province［J］. Rock and Mineral Analysis, 2014, 33(5): 737-746.)
［4］池汝安, 田君. 风化壳淋积型稀土矿评述［J］. 中国稀土学报, 2007, 25(6): 641-650. (Chi R A, Tian J. Review of weathered crust rare earth ore［J］. Journal of the Chinese Rare Earth Society, 2007, 25(6): 641-650.)
［5］金姝兰，黄益宗. 稀土元素对农田生态系统的影响研究进展［J］. 生态学报, 2013, 33(16): 4836-4845. (Jin S L, Huang Y Z. A review on rare earth elements in farmland ecosystem［J］. Acta Ecologica Sinica, 2013, 33(16): 4836-4845.)
［6］连腾祥. 大豆光合碳在黑土中的转化过程及细菌群落特征［D］. 长春: 中国科学院研究生院(中国科学院东北地理与农业生态研究所), 2016: 53-66. (Lian T X. The turnover of photosynthetic carbon of soybean and relevant bacterial community characteristics in the Mollisols［D］. Changchun: Northeast Institute of geography and Agroecology, Chinese Academy of Sciences, 2016: 53-66.)
［7］陈雪丽, 王玉峰, 李伟群, 等. 黑土区连作大豆根际微生物群落结构的动态变化［J］. 大豆科学, 2018, 37(5): 748-755. (Chen X L, Wang Y F, Li W Q, et al. Dynamic evolution of microbial community in the rhizosphere of continuous cropping of soybean in black soil［J］. Soybean Science, 2018, 37(5):748-755.)
［8］郭慧娟. 大豆快生根瘤菌共生基因的遗传分化及快慢生根瘤菌对土壤微生物的影响比较［D］. 北京: 中国农业大学, 2014: 1-8. (Guo H J. Replicon-dependent differentiation of symbiosis-related genes in Sinorhizobium strains and the effect on soil microbial community of Sinorhizobium and Bradyrhizobium［D］. Beijing: China Agricultural University, 2014: 1-8.)
［9］关大伟, 李力, 姜昕, 等. 长期施肥对黑土大豆根瘤菌群体结构和多样性的影响［J］. 生物多样性, 2015, 23(1): 68-78. (Guan D W, Li L, Jiang X, et al. Influence of long-term fertilization on the community structure and diversity of soybean rhizobia in black soil［J］. Biodiversity Science, 2015, 23(1): 68-78.)
［10］Poly F, Monrozier L J, Bally R. Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil［J］. Research in Microbiology, 2001, 152(1): 95-103.
［11］沈建秀. 接种根瘤菌后刺槐对镉胁迫的响应［D］. 杨凌: 西北农林科技大学, 2017: 16-30. (Shen J X. Response of Robinia pseudoacacia to cadmium stress after inoculation with rhizobium［D］. Yangling: Northwest A&F University, 2017: 16-30.)
［12］Jin Y, Liu H, Luo D, et al. DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways［J］. Nature Communications, 2016, 7: 12433.
［13］Zehr J P, Jenkins B D, Short S M, et al. Nitrogenase gene diversity and microbial community structure: A cross-system comparison［J］. Environ Microbiol, 2003, 5(7): 539-554.
［14］唐凤灶. 安徽铜陵铜尾矿原生演替过程中的土壤固氮菌研究［D］. 广州: 中山大学, 2010: 5-30. (Tang F Z. Soil nitrogen-fixing microorganisms in primary succession of copper mine tailings at Tongling, Anhui, China［D］. Guangzhou: Sun Yat-sen University, 2010: 5-30.)
［15］姚钦. 生物炭施用对东北黑土土壤理化性质和微生物多样性的影响［D］. 长春：中国科学院大学(中国科学院东北地理与农业生态研究所), 2017: 21-39. (Yao Q. Effect of biochar addition on soil physicochemical properties and microbial diversity in a black soil of northeast China［D］. Changchun: Northeast Institute of geography and Agroecology, Chinese Academy of Sciences, 2017: 21-39.)
［16］Kolton M, Graber E R, Tsehansky L, et al. Biochar-stimulated plant performance is strongly linked to microbial diversity and metabolic potential in the rhizosphere［J］. New Phytologist, 2017, 213(3): 1393-1404.
［17］唐行灿，陈金林. 生物炭对土壤理化和微生物性质影响研究进展［J］. 生态科学, 2018, 37(1): 192-199. (Tang X C, Chen J L. Review of the effect of biochar on soil physi-chemical and microbial properties［J］. Ecological Science, 2018, 37(1): 192-199.)
［18］何莉莉，杨慧敏，钟哲科，等. 生物炭对农田土壤细菌群落多样性影响的PCR-DGGE分析［J］. 生态学报, 2014, 34(15): 4288-4294. (He L L, Yang H M, Zhong Z K, et al. PCR-DGGE analysis of soil bacterium community diversity in farmlandinfluenced by biochar［J］. Acta Ecologica Sinica, 2014, 34(15): 4288-4294.)
［19］Wu J, Wang J, Zhang X, et al. A gyrB-targeted PCR for rapid identification of Paenibacillus mucilaginosus［J］. Applied Microbiology and Biotechnology, 2010, 87(2): 739-747.
［20］Ma M, Wang Z, Li L, et al. Complete genome sequence of Paenibacillus mucilaginosus 3016, a bacterium functional as microbial fertilizer［J］. Journal of Bacteriology, 2012, 194(10): 2777-2778.
［21］李敏. 铅镉污染土壤的稳定化及蚯蚓联合植物修复［D］. 上海: 华东师范大学, 2018: 45-54. (Li M. Stabilization and earthworm-assisted phytoremediation of Pb-Cd contaminated soil［D］. Shanghai: East China Normal University, 2018: 45-54.)
［22］李彦霈，邵明安，王娇. 蚯蚓粪施用方式及用量对土壤入渗的影响［J/OL］. 土壤学报, DOI:10.11766/trxb201807250261. (Li Y P, Shao M A, Wang J. Effects of pattern and amount of earthworm cast application on water infiltration in soil［J/OL］. Acta Pedologica Sinica, DOI:10.11766/trxb201807250261.)
［23］Peix A, Ramirez-Bahena M H, Velazquez E, et al. Bacterial associations with legumes［J］. Critiacal Reviews in Plant Sciences, 2015, 34(1-3SI): 17-42.
［24］Clua J, Roda C, Eugenia Z, et al. Compatibility between legumes and rhizobia for the establishment of a successful nitrogen-fixing symbiosis［J］. Genes, 2018, 9(3): 125.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

收稿日期：2018-10-11

基金项目：中央财政林业示范推广项目［(2015)GDTK-08］；广东省林业科技创新项目(2017KJCX033)；广东省科技计划(2015A020209139，2015B020207002)；广东省大学生创新训练项目（201710564034）。
第一作者简介：刘明骞（1987-），男，博士，实验师，主要从事植物遗传与应用研究。E-mail：liumq@scau.edu.cn。
通讯作者：陈晓阳（1958-），男，博士，教授，主要从事林木遗传育种研究。E-mail：xychen@scau.edu.cn。

更新日期/Last Update: 2019-01-22