[1]王学敏,王晗,王超,等.线虫胁迫下大豆肌醇加氧酶活力研究[J].大豆科学,2018,37(06):928-931.[doi:1011861/jissn1000-98412018060928]
　WANG Xue-min,WANG Han,WANG Chao,et al.Study on Activity of Myo-inositol Oxygenase Under Stress of Nematodes [J].Soybean Science,2018,37(06):928-931.[doi:1011861/jissn1000-98412018060928]
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线虫胁迫下大豆肌醇加氧酶活力研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年06期 页码: 928-931 栏目: 出版日期: 2018-11-20
Title:
Study on Activity of Myo-inositol Oxygenase Under Stress of Nematodes
作者:
（1.沈阳农业大学 生物科学技术学院，辽宁 沈阳 110866； 2.黑龙江省农业科学院，黑龙江 哈尔滨 1500862； 3.沈阳农业大学 植物保护学院，辽宁 沈阳 110866）
Author(s):
WANG Xue-min1; WANG Han1; WANG Chao1; WANG Shuang1; YU Bai-shuang2; CHEN Jing-sheng2; WANG Hui1; DUAN Yu-xi3
（1.College of Bioscience and Biotechnology， Shenyang Agricultural University，Liaoning 110866，China；2.Heilongjiang Academy of Agricultural Sciences，Harbin 150086， China；3.College of Plant Protection，Shenyang Agricultural University，Shenyang 110866，China）
关键词:
大豆; 肌醇加氧酶; 大豆胞囊线虫; 酶活力
Keywords:
Soybean; MIOX; Heterodera glycines; Enzyme activity
DOI:
1011861/jissn1000-98412018060928
文献标志码:
A
摘要:
为确定大豆胞囊线虫侵染大豆根部后，肌醇加氧酶活力的变化，以感病品种辽豆15和抗病品种灰皮支黑豆、哈尔滨小黑豆和小粒黑豆为试验材料，在人工接种大豆胞囊线虫3号生理小种后，利用肌醇加氧酶(MIOX)酶联免疫试剂盒检测肌醇加氧酶活力的变化情况。结果表明，在接种后的第5天，感病品种辽豆15酶活力达到最大值，约为对照组的161倍。而抗病品种哈尔滨小黑豆、灰皮支黑豆和小粒黑豆分别在接种后的第10天、第15天、第5天肌醇加氧酶活力达到最高，分别是对照组酶活力的146，151，185倍。
Abstract:
Inorder to determine the activity of Myo-inositol oxygenase after soybean cyst infected soybean rootA susceptible cultivar Liaodou 15 and the resistant cultivars Huipizhi heidou, Harbin Xiaoheidou and Xiaoliheidou were used inositol oxygenase (MIOX) elisa kit to detect changes of the inositol oxygenase enzyme activity from soybeans roots after SCN3 infection The results showed that 5 d after inoculation, Liaodou15 cultivars enzyme activity reached the maximum value, which was about 161 times in the control groupAnd disease-resistant cultivars of Harbin Xiaoheidou and Xiaoliheidou and Huipizhi Heidou, in the 10, 15, 5 d after inoculation, inositol oxygenase reached the highest enzyme activity were the control enzyme activity of 146 times, 151 times, 185 times respectively

参考文献/References:

［1］吴海燕, 远方, 陈立杰, 等 大豆胞囊线虫病与大豆抗胞囊线虫机制的研究［J］. 大豆科学, 2001(4): 285-289(Wu H Y,Yuan F,Chen L J,et al.Advances in soybean cyst nematode and mechenism of soybean resistance to Heterode a glycines［J］.Soybean Science,2001(4):285-289)
［2］Wrather J A, Stienstra W C, Koenning S R Soybean disease loss estimates for the United States from 1996 to 1998［J］. Canadian Journal of Plant Pathology, 2001, 23(2): 122-131
［3］Endres S, Tenhaken R Myoinositol oxygenase controls the level of myoinositol in Arabidopsis, but does not increase ascorbic acid［J］. Plant Physiology, 2009, 149(2): 1042-1049
［4］Michell R H Inositol derivatives: Evolution and functions［J］. Nature Reviews Molecular Cell Biology, 2008, 9(2): 151-161
［5］Michell R H Evolution of the diverse biological roles of inositols ［J］ Biochemical Society Symposium,2007,74:223-246
［6］Hinterberg B, Klos C,Tenhaken R Recombinant UDP-glucose dehydrogenase from soybean［J］. Plant Physiology and Biochemistry,2002, 40(12):1011-1017
［7］Alford S RMolecular characterization of two myo-inositol oxygenases in Arabidopsis thaliana［D］. Blacksburg ：Virginia Polytechnic Institute and State University,2009
［8］都浩水稻中激素和肌醇磷酸代谢相关基因的抗逆功能研究［D］.武汉：华中农业大学, 2013(Du H Hormones and inositol phosphate metabolism related genes in rice research art function［D］.Wuhan:Huazhong Agricultural University,2013)
［9］Lorence A, Chevone B I, Mendes P, et al. Myo-inositol oxygenase offers a possible entry point into plant ascorbate biosynthesis［J］. Plant Physiology,2004,134(3):1200-1205
［10］Schoenfeld J D, Sibenaller Z A, Mapuskar K A, et al.O2-and H2O2-mediated disruption of Fe metabolism causes the differential susceptibility of NSCLC and GBM cancer cells to pharmacological ascorbate［J］. Cancer Cell,2017,31(4): 487-500
［11］Michell R H Evolution of the diverse biological roles of inositols［J］. Biochemical Society Symposium, 2007,74(2):223-246
［12］Loewus F A, Loewus M W Myo-inositol: Its biosynthesis and metabolism［J］. Annual Review of Plant Physiology,1983, 34(1):137-161
［13］Loewus F A,Murthy P P Myo-Inositol metabolism in plants［J］. Plant Science,2000,150(1):1-19
［14］Gillaspy G E, Ercetin M E, Burnette R NInositol metabolism in plant cells: A genomics perspective［J］. Advances in Plant Physiology,2004, 7: 145-158
［15］Gillaspy G E The cellular language of myo-inositol signaling［J］. New Phytologist,2011,192(4): 823-839
［16］Donahue J L, Alford S R, Tora binejad J, et al. The Arabidopsis thalianamyo-inositol 1-phosphate synthase1 gene is required for myo-inositolsynthesis and suppression of cell death［J］. Plant Cell, 2010, 22(2):888-903
［17］Arner R J,Prabhu K S,Thompson J T,et al. Myo-Inositol oxygenase: Molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and D-chiro-inositol［J］. Biochemical Journal, 2001,360(2): 313-320
［18］Moskala R, Reddy C C, Minard R D, et al. An oxygen-18 tracer investigation of the mechanism of myo-inositol oxygenase［J］. Biochemical and Biophysical Research Communications,1981, 99(1): 107-113
［19］Kanter U, Usadel B, Guerineau F,et al. The inositol oxygenase gene family of Arabidopsis is involved in the biosynthesis of nucleotide sugar precursors for cell-wall matrix polysaccharides［J］. Planta, 2005,221(2):243-254
［20］Endres S, Tenhaken R Myo-inositol oxygenase controls the level of myo-inositol in Arabidopsis, brackcross progeny to entry and development of heterodera glycines［J］.Plant Physiology ,2011,149:788-798
［21］Endres S, and Tenhaken R Myo-inositol oxygenase controls the level of myo-inositol in Arabidopsis,but does not increase ascorbic acid［J］.Plant Physiol,2009,149:1042-1049

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备注/Memo

收稿日期：2018-08-20

基金项目：国家自然科学基金重点项目资助（31330063）。
第一作者简介：王学敏（1992-），男，硕士，主要从事大豆胞囊线虫抗性基因相关研究。E-mail: 2528745520@qqcom。
通讯作者：王惠（1972-）女，博士，副教授，主要从事大豆抗病、抗逆机制和分子生物学研究。E-mail:wanghuisyau@sinacom。
段玉玺（1964-），男，博士，教授，主要从事植物病理学和植物线虫学研究。E-mail:duanyx6407@163com。

更新日期/Last Update: 2018-12-04