[1]张玉梅,胡润芳,林国强.菜用大豆蔗糖转运蛋白生物信息学分析[J].大豆科学,2018,37(06):866-870.[doi:1011861/jissn1000-98412018060866]
　ZHANG Yu-mei,HU Run-fang,LIN Guo-qiang.Bioinformatics Analysis of Vegetable Soybean Sucrose Transporter [J].Soybean Science,2018,37(06):866-870.[doi:1011861/jissn1000-98412018060866]
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菜用大豆蔗糖转运蛋白生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年06期 页码: 866-870 栏目: 出版日期: 2018-11-20
Title:
Bioinformatics Analysis of Vegetable Soybean Sucrose Transporter
作者:
（福建省农业科学院 作物研究所，福建 福州 350013）
Author(s):
ZHANG Yu-mei;  HU Run-fang;  LIN Guo-qiang
(Crop Sciences Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China)
关键词:
菜用大豆; 蔗糖转运蛋白; 生物信息学分析; GmSUT基因
Keywords:
Vegetable soybean; SUT; Bioinformatics analysis;  GmSUT
DOI:
1011861/jissn1000-98412018060866
文献标志码:
A
摘要:
为揭示菜用大豆SUT基因的结构特点，并为提高其表达活性提供理论依据，利用RT-PCR技术克隆菜用大豆SUT基因，长度为1 634 bp，编码区1 566 bp，编码一个含有521个氨基酸残基的多肽。生物信息学分析表明，SUT理论等电点933，理论分子量5519 kD，是疏水性蛋白。SOMPA分析表明，SUT各个氨基酸残基对应的二级结构为α螺旋4511%、β折叠1420%、无规则卷曲3647%和β转角422%；氨基酸序列和结构分析显示SUT蛋白包含1个保守区，即MFS_2 domain和12个跨膜区。SignalP分析表明该蛋白没有信号肽。系统进化树分析表明菜用大豆GmSUT蛋白与绿豆(Vignaradiata var radiata)的亲缘关系最近，同源性达9328%。将该蛋白与拟南芥的9个SUC蛋白比对表明GmSUT与AtSUC2的亲缘关系最近，属于SUT1亚族。
Abstract:
In order to reveal the construction features of vegetable soybean sucrose transporter in the molecular level and provide a theoretical basis for increasing the activity of its expression．Soybean SUT gene whose sequence is 1 634 bp in length was cloned by RT-PCR and the sequence in coding region of SUT is 1 566 bp，a polypeptide was encoded by 521 amino acid residues．Bioinformatics analysis showed that the theory value of pI and Mw was 933 and 5519 kD, respectively SUT was a hydrophobic protein The secondary structure of SUT various amino acid residues were predicted by SOMPA, which was as follows alpha helix accounting for 4511%, extended strand for 1420%, random coil for 3647% and β-turn for 422% According to the amino acid sequence and structural analysis，it showed that SUT protein contained one conserved domain (MFS_2 domain) and twelve transmembrane regions This protein was no signal peptide analysised by SignalP Phylogenetic tree analysis showed that the GmSUT had the closest genetic relationship with Vignaradiata var radiata and the homology was 9328% The alignment between this protein and 9 SUC proteins of Arabidopsis thaliana showed that GmSUT was closely related to AtSUC2 and belonged to the SUT1 subfamily

参考文献/References:

［1］Barker L,Kühn C, Weise A, et al. SUT2, a putative sucrose sensor in sieve element ［J］. Plant Cell, 2000(12):1153-1164
［2］Williams L E, Lemoine R, Sauer N Sugar transporters inhigher plants-a diversity of roles and complex regulation ［J］.Trends in Plant Science, 2000, 5(7):283-290
［3］Hirose T, Zhang Z, Miyao A, et al. Disruption of a gene for rice sucrose transporter, OsSUT1, impairs pollen function but pollen maturation is unaffected［J］ Journal of Experimental Botany, 2010, 61(13):3639-3646
［4］Kühn C, Grof C P L Sucrose transporters of higher plants ［J］. Current Opinion in Plant Biology, 2010, 13(3):288-298
［5］Aoki N, Hirose T, Scofield G N, et al. The sucrose transporter gene family in rice ［J］. Plant and Cell Physiology, 2003, 44(3): 223-232
［6］Ibraheem O, Dealtry G, Roux S, et al. The effect of drought and salinity on the expressional levels of sucrose transporters in rice (Oryza sativa Nipponbare) cultivar plants ［J］. Plant Omics Journal, 2011, 4(2):68-74
［7］Mimura M, Coyne C J, Bambuck M W, et al. SSR diversity of vegetablesoybean ［Glycine max (L) Merr］ ［J］. Genetic Resources and Crop Evolution, 2007, 54:497-508
［8］Czaikoski K, Leite R S, Mandarino J M G, et al. Canning of vegetable-type soybean in acidified brine: Effect of the addition of sucrose and pasteurisation time on color and other characteristics ［J］. Industrial Crops and Products, 2013, 45:472-476
［9］Kim S Y, Kim B M, Kim J B, et al. Effect of steaming, blanching, and high temperature/ high pressure processing on the amino acid contents of commonly consumed Korean vegetables and pulses［J］. Prevention Nutrition and Food Science,2014, 19:220-226
［10］徐有,王凤敏,默邵景,等我国菜用大豆的研究现状与发展趋势［J］.河北农业科学,2012,16(4):42-45(Xu Y, Wang F M, Mo S J, et al. Research status and development tendency of vegetable soybean in China ［J］. Journal of Hebei Agricultural Sciences, 2012,l6(4):42-45)
［11］张彩英,常文锁,李喜焕,等种植密度和施肥对莱用大豆产量性状的效应研究［J］.中国农学通报,2005,21(7):190-192(Zhang C Y, Chang W S, Li X H, et al. Effect of plant density and phosphorus application rate in yield of vegetable soybean ［J］. Chinese Agricultural Science Bulletin, 2005,21(7):190-192)
［12］张玉梅, 赵晋铭, 王明军, 等 南方菜用大豆资源营养品质性状的遗传变异［J］. 大豆科学,2006, 25(3):239-243 (Zhang Y M, Zhao J M, Wang M J, et al. Genetic variance of nutritional quality of vegetbale soybean germplasm of Glycine max Merr in southern China ［J］. Soybean Science, 2006, 25(3): 239-243)
［13］Li Y S, Ming D, Zhang Q Y , et al. Greater differences exist in seed protein, oil, total soluble sugar and sucrose content of vegetable soybean genotypes ［Glycine max (L) Merrill］ in Northeast China ［J］. Australian Journal of Crop Science, 2012, 6(12):1681-1686
［14］Petersen T N, Brunak S, Heijne V,et al.SignalP 40: Discriminating signal peptides from transmembrane regions［J］. Nature Methods, 2011,8(10):785-786
［15］Sauer N, Ludwig A, Knoblauch A, et al. AtSUC8 and AtSUC9 encode functional sucrose transporters but the closely related AtSUC6 and AtSUC7 genes encode aberrant proteins in different Arabidopsis ecotypes［J］.The Plant Journal, 2004, 40(1):120-130
［16］Vaughn M W, Harrington G N, Bush D R Sucrose-mediated transcriptional regulation of sucrose symporter activity in the phloem ［J］.Proceedings of the National Academy of Science of the United States of America, 2002, 99(16):10876-10880
［17］Srivastava A C, Ganesan S, Ismail I O, et al. Functional characterization of the Arabidopsis AtSUC2 sucrose/H+ symporter by tissue specific complementation reveals an essential role in phloem loading but not in long distance transport ［J］.Plant Physiology, 2008, 148(1):200-211
［18］Riesmeier J W, Willmitzer L, Frommer W B Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning ［J］. Embo Journal, 1994, 13(1):1-7
［19］Kühn C, Quick W P, Schulz A, et al. Companion cell-specific inhibition of the potato sucrose transporter SUT1 ［J］. Plant Cell Environment, 1996, 19(10):1115-1123
［20］Burkle L, Hibberd J M, Quick W P, et al. The H+-sucrose cotransporter NtSUT1 is essential for sugar export from tobacco leaves ［J］. Plant Physiology, 1998, 118(1):59-68
［21］张玉梅, 胡润芳, 陈宇华, 等 菜用大豆籽粒代谢物的相关性分析［J］. 大豆科学, 2018, 37(2):231-238 (Zhang Y M, Hu R F, Chen Y H, et al. Correlation analysis between metabolites in vegetable soybean seeds ［J］. Soybean Science, 2018, 37(2):231-238 )
［22］张秋英, 李彦生, 刘长锴, 等 菜用大豆食用品质关键组分及其积累动态研究［J］. 作物学报,2015, 41(11):1692-1700 (Zhang Q Y, Li Y S, Liu C K, et al. Key components of eating quality and their dynamic accumulation in vegetable soybean varieties ［Glycine max (L) Merr］ ［J］. Acta Agronomica Sinica,2015, 41(11): 1692-1700)
［23］Zhang Y M, Hu R F, Li H W, et al. Proteomics changes in filling seeds of vegetable soybean［J］. Hort Science, 2016, 51(11):1397-1401

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备注/Memo

收稿日期：2018-05-07

基金项目：福建省属公益类科研院所基本科研专项（2016R1025-6，2018R1026-1）；福建省科技重大专项（2015NZ0002-3）；福建省农业科学院科技创新团队资助项目（STIT2017-2-6）。
第一作者简介：张玉梅（1980-），女，博士，助理研究员，主要从事大豆新品种选育及栽培技术研究。E-mail:zym1122@126com。
通讯作者：林国强（1965-），男，学士，研究员，主要从事大豆新品种选育及栽培技术研究。E-mail: lgq308@163com。

更新日期/Last Update: 2018-12-04