[1]滕露,于月华,何茹月,等.大豆miR164家族的生物信息学分析[J].大豆科学,2018,37(05):704-709.[doi:10.11861/j.issn.1000-9841.2018.05.0704]
　TENG Lu,YU Yue-hua,HE Ru-yue,et al.Bioinformatics Analysis of Soybean miR164Family[J].Soybean Science,2018,37(05):704-709.[doi:10.11861/j.issn.1000-9841.2018.05.0704]
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大豆miR164家族的生物信息学分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年05期 页码: 704-709 栏目: 出版日期: 2018-09-20
Title:
Bioinformatics Analysis of Soybean miR164Family
作者:
(新疆农业大学 农学院，新疆 乌鲁木齐 830052)
Author(s):
TENG Lu;  YU Yue-hua;  HE Ru-yue;  CHEN Quan-jia;  NI Zhi-yong
(College of Agriculture, Xinjiang Agricultural University, Urumqi 831300，China)
关键词:
大豆; miR164家族; 生物信息学; 启动子分析
Keywords:
Soybean; MiR164family; Bioinformatics; Promoter analysis
DOI:
10.11861/j.issn.1000-9841.2018.05.0704
文献标志码:
A
摘要:
microRNA164是一个高度保守的miRNA家族，广泛参与植物的细胞和生理过程。本论文旨在采用生物信息学方法，了解大豆miR164基因家族在大豆生长发育及逆境胁迫应答过程中扮演的重要角色，为其在大豆的分子育种和品种改良方面的研究提供理论基础。利用miRNA、Plant MicroRNA Database、PLACE和NCBI数据库以及Clustal X2.0、MEGA 5.0和DNAMAN软件对gma-miR164基因家族的序列情况、染色体定位、靶基因调控功能、启动子元件、二级结构和系统发育树进行生物信息学分析。在miRBase中搜索到11条gma-miR164基因同源序列，分别分布在7条染色体上，其中以位于Chr3上的序列最多，共有3条，位于Chr10和Chr19上各有2条，而在Chr02、Chr09、Chr18和Chr20上各有1条。11个gma-miR164基因家族成员共预测到6个靶基因，均为NAC转录因子。gma-miR164基因家族成熟序列的碱基保守性很高，其前体序列均可形成稳定的二级茎环结构。启动子中顺式调控元件分析表明，ABRE、DRE、MYB和MYC这4个元件在gma-miR164基因家族启动子序列中分布不均，其中以MYB和MYC元件数目居多。本研究为探寻miR164基因家族在逆境胁迫应答中的调控作用提供了数据基础和理论依据。
Abstract:
MicroRNA164is a highly conserved miRNA family, widely involved in plant cell and physiological processes. The purpose of this study was to investigate the important role of miR164gene family in soybean growth and stress response by bioinformatics, and to provide a theoretical basis for molecular breeding and variety improvement in soybean. Using Plant MicroRNA Database，PLACE and NCBI Database, Clustal X2.0，MEGA5.0 and DNAMAN to analyze the sequence, chromosome location, target gene regulation function, promoter element, secondary structure and the phylogenetic tree was analyzed by bioinformatics. In the miRBase, 11 gma-miR164gene homologous sequences were searched and distributed on 7 chromosomes. There were 3 sequences in Chr3, two in Chr10 and two in Chr19, and one in Chr02，Chr09，Chr18 and Chr20. 11 members of gma-miR164gene family predicted 6 target genes, all of which were NAC transcription factors. The mature sequence of gma-miR164gene family was highly conserved, and its precursor sequence can form a stable secondary stem ring structure. The analysis of cis-regulatory elements in promoter showed that the four elements of ABRE, DRE, MYB and MYC were found in gma-miR164gene. The family promoter sequences were unevenly distributed, in which the number of MYB and MYC elements was the majority. This study provides a data basis and theoretical basis for exploring the regulatory role of miR164family in stress response.

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备注/Memo

收稿日期：2018-06-04

基金项目：国家自然科学基金（31660295）；中国博士后科学基金（2015M582741）；新疆维吾尔自治区天山英才计划（201720085）；新疆农业大学校级大学生创新项目（dxscx2017001，dxscx2018001）。
第一作者简介：滕露（1995-），女，硕士，主要从事植物分子生物学研究。E-mail：894546653@qq.com。
通讯作者：倪志勇（1981-），男，博士，副教授，主要从事植物分子生物学研究。E-mail：nizhiyong@126.com。

更新日期/Last Update: 2018-10-08