[1]王昭玉,甄珍,李雅琳,等.大豆转录因子GmWRKY4分子克隆与表达分析[J].大豆科学,2018,37(04):539-544.
　WANG Zhao-yu,ZHEN Zhen,LI Ya-lin,et al.Cloning and Expression Analysis of Transcription Factors GmWRKY4 in Soybean[J].Soybean Science,2018,37(04):539-544.
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大豆转录因子GmWRKY4分子克隆与表达分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年04期 页码: 539-544 栏目: 出版日期: 2018-07-20
Title:
Cloning and Expression Analysis of Transcription Factors GmWRKY4 in Soybean
作者:
王昭玉1; 甄珍1; 李雅琳1; 王翠霞2; 刘思敏1; 郭芸彤1; 闫瑞娴1; 刘建凤1
(1.河北大学 生命科学学院，河北 保定 071002； 2.冀州区农牧局，河北 衡水 053200)
Author(s):
WANG Zhao-yu1; ZHEN Zhen1; LI Ya-lin1; WANG Cui-xia2; LIU Si-min1; GUO Yun-tong1; YAN Rui-xian1; LIU Jian-feng1
(1.College of Life Science, Hebei University,Baoding 071002,China; 2.Agriculture and Animal Husbandry Bureau of Jizhou District,Hengshui 053200,China)
关键词:
大豆; GmWRKY4; 生物信息学; 非生物胁迫; 激素诱导
Keywords:
Glycine max; GmWRKY4; Bioinformatics;  Abiotic stres; Hormone induction
文献标志码:
A
摘要:
为研究WRKY家族基因在大豆抵御非生物胁迫中的生物学功能及其作用机制，利用铁丰29，获得了大豆WRKY家族基因GmWRKY4的开放阅读框序列，并对其在不同非生物胁迫处理下的表达模式进行了分析。结果表明，GmWRKY4开放阅读框为1 083 bp、编码360个氨基酸残基；对其编码蛋白GmWRKY4进行保守域及同源性分析发现，属于WRKY转录因子家族第1类，与GsWRKY4高度同源；分析GmWRKY4与拟南芥WRKY转录因子系统进化树发现，与AtWRKY3、AtWRKY4相似性最高；进一步分析该基因在盐（NaCl处理）、干旱（PEG处理）与低温胁迫处理下的表达模式发现， GmWRKY4可参与对上述3种非生物胁迫的响应；同时分析基因在乙酰辅酶A羧化酶（ACC）、水杨酸（SA）、茉莉酸（JA）等激素处理下的表达模式发现，GmWRKY4可通过参与ACC、SA、JA信号通路实现对逆境胁迫的响应，为进一步深入研究该基因生物学功能及其作用机制奠定了基础。
Abstract:
In order to understand how WRKY gene family participates in soybean defensive responses and regulating the growth and development of soybean, Tiefeng 29 was used as the research material to obtain the open reading frame (ORF)1 083 bp of soybean gene GmWRKY4, which encoded 360 amino acid polypeptide. Further bioinformatics analysis revealed that the amino acid sequence of GmWRKY4 had two structural conserved domains (WRKY) and C2H2 type zinc finger structure, so it belonged to the class I of gene family. The homology of amino acid sequence between GmWRKY4 and GsWRKY was 99%. The phylogenetic tree analysis showed that the GmWRKY4, Arabidopsis AtWRKY3 and AtWRKY4 belonged to the same class. It was also found that the GmWRKY4 was involved in responses to different stress, including NaCl, PEG, low-tempture and ACC,SA,JA induction. This study would lay a foundation for the family structure and functional research of soybean WRKY transcription factors, and further provide scientific basis for research of soybean resistance and molecular breeding.

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备注/Memo

收稿日期：2018-02-05

基金项目：河北大学生命科学学院本科教学项目。
第一作者简介：王昭玉（1992-），女，硕士，主要从事植物分子生物学研究。E-mail：1023988344@qq.com。
通讯作者：刘建凤（1979-），女，博士，副教授，主要从事植物逆境胁迫机制研究。E-mail: jianfengliu@hbu.edu.cn。

更新日期/Last Update: 2018-07-31