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[1]张立军,孙旭刚,李盛有,等.国内外大豆种质耐荫性综合评价及相关鉴定指标的筛选[J].大豆科学,2018,37(04):493-502.[doi:10.11861/j.issn.1000-9841.2018.04.0493]
　ZHANG Li-jun,SUN Xu-gang,LI Sheng-you,et al.Comprehensive Evaluation for Shade-Tolerant and Selection of Identification Indicators in Soybean Germplasms from China and Abroad[J].Soybean Science,2018,37(04):493-502.[doi:10.11861/j.issn.1000-9841.2018.04.0493]

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国内外大豆种质耐荫性综合评价及相关鉴定指标的筛选

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷期数: 2018年04期页码: 493-502 栏目: 出版日期: 2018-07-20

Title:: Comprehensive Evaluation for Shade-Tolerant and Selection of Identification Indicators in Soybean Germplasms from China and Abroad

作者:: 张立军; 孙旭刚; 李盛有; 宋书宏; 王文斌; 曹永强; （辽宁省农业科学院作物研究所，辽宁沈阳 110161）

Author(s):: ZHANG Li-jun; SUN Xu-gang; LI Sheng-you; SONG Shu-hong; WANG Wen-bin; CAO Yong-qiang; (Crop Institute of Liaoning Academy of Agricultural Sciences, Shenyang 110161,China)

关键词:: 大豆; 耐荫性; 主成分分析; 隶属函数法; 聚类分析; 逐步回归

Keywords:: Soybean; Shade-tolerance; Principal components analysis; Membership function; Cluster analysis; Stepwise regression analysis

DOI:: 10.11861/j.issn.1000-9841.2018.04.0493

文献标志码:: A

摘要:: 为探讨大豆耐荫胁迫评价方法，筛选鉴定大豆耐荫性状指标，建立科学可靠的耐荫性评价的数学模型，采用盆栽的方法，设遮光和自然光照2个处理，成熟期考种测量株高（X1）、主茎节数（X2）、节间长度（X3）、分枝数（X4）、单株荚数（X5）、单株生物量（X6）、单株产量（X7）、表观收获指数（X8）及百粒重（X9），对82份国内外大豆种质资源进行耐荫性评价，并根据耐荫性数学评价模型确定合理高效的耐荫性鉴定指标。结果表明：通过主成分分析将9个单项性状转换为4个相互独立的综合指标，代表了全部数据 83.155%的信息量；通过隶属函数法计算综合耐荫评价值（D），并进行聚类分析，将82份国内外大豆种质按照耐荫性的强弱划分强耐荫、中度耐荫和弱耐荫3种类型，筛选出14个强耐荫型大豆种质；通过逐步回归建立大豆耐荫性评价数学模型，D=0.024+0.148X²-0.094X³-0.088X⁵+0.408X⁷+0.236X⁹（R²=0.997），并筛选出5个鉴定指标，分别是主茎节数、节间长度、单株荚数、单株粒重和百粒重；综合聚类和逐步回归结果发现，强耐荫型大豆种质在遮光处理条件下具有较多的主茎节数，较短的节间长度和较高的单株荚数和单株产量。

Abstract:: Abstract: In order to evaluate the methods of shade-tolerant in soybean, screen and identify the soybean shade tolerance traits, and establish a scientific and reliable mathematical model of shade tolerance evaluation, 82 soybean germplasms from China and abroad were treated under shading and natural light conditions in the pot-culture experiments, and the plant height (X1), main stem node number (X2), internode length (X3), branch number (X4), pods per plant (X5), biomass per plant (X6), yield per plant (X7), apparent harvest index (X8) and 100-seed weight (X9) were investigated at the mature period to evaluate the shade-tolerant and confirm the suitable and efficient indicators of shade-tolerance according to mathematical evaluation model. The results showed that the nine single indexes were transformed into four independent comprehensive components by principal component analysis, which represent 83.155% information of the raw data. The membership function analysis was used to calculate comprehensive shade-tolerance value (D), and 82 landraces were divided into three types including high shade-tolerant, medium shade-tolerant and shade-intolerant through cluster analysis for D value. A mathematical evaluation model for soybean shade-tolerant germplasms was established by stepwise regression analysis, D=0.024+0.148X2-0.094X3-0.088X5+0.408X7+0.236X9 (R2=0.997). Based on the model, five indexes were closely related to the shade-tolerance were screened out, including main stem nodes, internode length, pods per plant, yield per plant and 100-seed weight. That is to say, the high shade-tolerance types have more main stem nodes, shorter internode length, greater pods per plant and yield per plant.Keywords:

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备注/Memo

收稿日期：2018-04-02基金项目：国家重点研发计划（2016YFD0100201-1，2017YFD0101304-2）；国家现代农业产业技术体系（CARS-004-CES11）；辽宁省中央引导地方科技发展专项（2017108006）。第一作者简介：张立军（1980-），男，学士，助理研究员，主要从事大豆遗传育种与栽培研究。E-mail：zhanglj_8005@163.com。通讯作者：曹永强（1977-），男，博士，研究员，主要从事大豆遗传育种与栽培研究。E-mail：yongqiangcao@hotmail.com； 王文斌（1968-），男，硕士，研究员，主要从事大豆遗传育种与栽培研究。E-mail：wwbin@163.com。

更新日期/Last Update: 2018-07-25